A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species.
Plasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0220184 |
| _version_ | 1828954498260795392 |
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| author | Maud Combier Edouard Evangelisti Marie-Christine Piron David Rengel Ludovic Legrand Liron Shenhav Olivier Bouchez Sebastian Schornack Pere Mestre |
| author_facet | Maud Combier Edouard Evangelisti Marie-Christine Piron David Rengel Ludovic Legrand Liron Shenhav Olivier Bouchez Sebastian Schornack Pere Mestre |
| author_sort | Maud Combier |
| collection | DOAJ |
| description | Plasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-domains but lack the characteristic RXLR motif reported previously from oomycete effectors. We cloned four candidates and showed that one of them, Pv33, induces plant cell death in grapevine and Nicotiana species. This activity is dependent on the nuclear localization of the protein. Sequence similar effectors were present in seven European, but in none of the tested American isolates. Together our work contributes a new type of conserved P. viticola effector candidates. |
| first_indexed | 2024-12-14T07:30:35Z |
| format | Article |
| id | doaj.art-ab3f2b7c6b4840d2b8c25e16b5d85199 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:30:35Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ab3f2b7c6b4840d2b8c25e16b5d851992022-12-21T23:11:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e022018410.1371/journal.pone.0220184A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species.Maud CombierEdouard EvangelistiMarie-Christine PironDavid RengelLudovic LegrandLiron ShenhavOlivier BouchezSebastian SchornackPere MestrePlasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-domains but lack the characteristic RXLR motif reported previously from oomycete effectors. We cloned four candidates and showed that one of them, Pv33, induces plant cell death in grapevine and Nicotiana species. This activity is dependent on the nuclear localization of the protein. Sequence similar effectors were present in seven European, but in none of the tested American isolates. Together our work contributes a new type of conserved P. viticola effector candidates.https://doi.org/10.1371/journal.pone.0220184 |
| spellingShingle | Maud Combier Edouard Evangelisti Marie-Christine Piron David Rengel Ludovic Legrand Liron Shenhav Olivier Bouchez Sebastian Schornack Pere Mestre A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. PLoS ONE |
| title | A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. |
| title_full | A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. |
| title_fullStr | A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. |
| title_full_unstemmed | A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. |
| title_short | A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species. |
| title_sort | secreted wy domain containing protein present in european isolates of the oomycete plasmopara viticola induces cell death in grapevine and tobacco species |
| url | https://doi.org/10.1371/journal.pone.0220184 |
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