Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot
Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2388 |
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| author | Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli |
| author_facet | Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli |
| author_sort | Alessandro Passera |
| collection | DOAJ |
| description | Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusarium ear rot, and whether this trait could be related to particular compositions of the bacterial microbiota in the embryo, using different approaches. Our cultivation-independent approach utilized the metabarcoding of a portion of the 16S rRNA gene to study bacterial populations in these samples. Multivariate statistical analyses indicated that the microbiota of the embryos of the accessions grouped in two different clusters: one comprising three landraces and the hybrid, one including the remaining two landraces, which showed a lower susceptibility to fusarium ear rot in field. The main discriminant between these clusters was the frequency of Firmicutes, higher in the second cluster, and this abundance was confirmed by quantification through digital PCR. The cultivation-dependent approach allowed the isolation of 70 bacterial strains, mostly Firmicutes. In vivo assays allowed the identification of five candidate biocontrol strains against fusarium ear rot. Our data revealed novel insights into the role of the maize embryo microbiota and set the stage for further studies aimed at integrating this knowledge into plant breeding programs. |
| first_indexed | 2024-03-10T05:15:00Z |
| format | Article |
| id | doaj.art-ab413c4eac9b4a2b820e78ffb6420047 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ab413c4eac9b4a2b820e78ffb64200472023-11-23T00:30:43ZengMDPI AGMicroorganisms2076-26072021-11-01911238810.3390/microorganisms9112388Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear RotAlessandro Passera0Alessia Follador1Stefano Morandi2Niccolò Miotti3Martina Ghidoli4Giovanni Venturini5Fabio Quaglino6Milena Brasca7Paola Casati8Roberto Pilu9Davide Bulgarelli10Department of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyInstitute of Sciences of Food Production, Italian National Research Council, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyInstitute of Sciences of Food Production, Italian National Research Council, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences–Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milan, ItalyPlant Sciences, School of Life Sciences, University of Dundee, Invergowrie DD2 5DA, UKLocally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusarium ear rot, and whether this trait could be related to particular compositions of the bacterial microbiota in the embryo, using different approaches. Our cultivation-independent approach utilized the metabarcoding of a portion of the 16S rRNA gene to study bacterial populations in these samples. Multivariate statistical analyses indicated that the microbiota of the embryos of the accessions grouped in two different clusters: one comprising three landraces and the hybrid, one including the remaining two landraces, which showed a lower susceptibility to fusarium ear rot in field. The main discriminant between these clusters was the frequency of Firmicutes, higher in the second cluster, and this abundance was confirmed by quantification through digital PCR. The cultivation-dependent approach allowed the isolation of 70 bacterial strains, mostly Firmicutes. In vivo assays allowed the identification of five candidate biocontrol strains against fusarium ear rot. Our data revealed novel insights into the role of the maize embryo microbiota and set the stage for further studies aimed at integrating this knowledge into plant breeding programs.https://www.mdpi.com/2076-2607/9/11/2388<i>Fusarium verticillioides</i>16S metabarcodingdigital PCRRAPDFirmicutes |
| spellingShingle | Alessandro Passera Alessia Follador Stefano Morandi Niccolò Miotti Martina Ghidoli Giovanni Venturini Fabio Quaglino Milena Brasca Paola Casati Roberto Pilu Davide Bulgarelli Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot Microorganisms <i>Fusarium verticillioides</i> 16S metabarcoding digital PCR RAPD Firmicutes |
| title | Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot |
| title_full | Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot |
| title_fullStr | Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot |
| title_full_unstemmed | Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot |
| title_short | Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot |
| title_sort | bacterial communities in the embryo of maize landraces relation with susceptibility to fusarium ear rot |
| topic | <i>Fusarium verticillioides</i> 16S metabarcoding digital PCR RAPD Firmicutes |
| url | https://www.mdpi.com/2076-2607/9/11/2388 |
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