Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity
A 9-week growth trial was carried out to assess the influence of replacing poultry by-product meal protein with conventional cottonseed meal protein (CCMP) or low gossypol cottonseed meal protein (LGCMP) on growth, feed utilization, gut micromorphology, and immunity of hybrid grouper (<i>Epine...
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MDPI AG
2022-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/15/1906 |
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| author | Misbah Irm Bo Ye Xiaoyi Wu Lina Geng Qinxiao Cai Lu Zhang Haoyun Zhai Zhiyu Zhou |
| author_facet | Misbah Irm Bo Ye Xiaoyi Wu Lina Geng Qinxiao Cai Lu Zhang Haoyun Zhai Zhiyu Zhou |
| author_sort | Misbah Irm |
| collection | DOAJ |
| description | A 9-week growth trial was carried out to assess the influence of replacing poultry by-product meal protein with conventional cottonseed meal protein (CCMP) or low gossypol cottonseed meal protein (LGCMP) on growth, feed utilization, gut micromorphology, and immunity of hybrid grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>) juveniles fed low-fish meal (18.53%, dry matter) diets. Eleven experimental diets were prepared. The control diet (PBMP) contained 46.15% poultry by-product meal protein. Both conventional cottonseed meal protein (CCMP) and low-gossypol cottonseed meal protein (LGCMP) were used in replacement ratios of 20, 40, 60, 80, and 100% of poultry by-product meal protein (PBMP) from the control diet, forming ten experimental diets (CCMP20, CCMP40, CCMP60, CCMP80, CCMP100, LGCMP20, LGCMP40, LGCMP60, LGCMP80, and LGCMP100). Results demonstrated that weight-gain percentage (WG%) was not different between different sources of cottonseed meal (CCMP and LGCMP). However, values of WG% significantly differed among different replacement levels, with CCMP80 and LGCMP40 having significantly higher values compared to other treatments. Fish fed CCMP80 and LGCMP40 exhibited higher protein efficiency ratios (PERs) than fish fed other experimental diets. The regression analysis from a second-order or third-order polynomial model based on WG% showed that the optimal PBMP replacement levels by CCMP and LGCMP are 74% and 33%, respectively. The whole-body lipid contents remarkably decreased as dietary CCMP or LGCMP inclusion levels increased. The relative mRNA expression of insulin-like growth factor-1(IGF-1) in liver was higher in fish fed CCMP80 and LGCMP40 diets compared to fish fed other diets. Generally, in low-FM diets of hybrid grouper, CCMP and LGCMP could replace 74% and 33% of PBMP, respectively. |
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| language | English |
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| spelling | doaj.art-ab41488b63ea4128b47010b8310f688d2023-12-03T12:25:41ZengMDPI AGAnimals2076-26152022-07-011215190610.3390/ani12151906Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and ImmunityMisbah Irm0Bo Ye1Xiaoyi Wu2Lina Geng3Qinxiao Cai4Lu Zhang5Haoyun Zhai6Zhiyu Zhou7State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, ChinaA 9-week growth trial was carried out to assess the influence of replacing poultry by-product meal protein with conventional cottonseed meal protein (CCMP) or low gossypol cottonseed meal protein (LGCMP) on growth, feed utilization, gut micromorphology, and immunity of hybrid grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>) juveniles fed low-fish meal (18.53%, dry matter) diets. Eleven experimental diets were prepared. The control diet (PBMP) contained 46.15% poultry by-product meal protein. Both conventional cottonseed meal protein (CCMP) and low-gossypol cottonseed meal protein (LGCMP) were used in replacement ratios of 20, 40, 60, 80, and 100% of poultry by-product meal protein (PBMP) from the control diet, forming ten experimental diets (CCMP20, CCMP40, CCMP60, CCMP80, CCMP100, LGCMP20, LGCMP40, LGCMP60, LGCMP80, and LGCMP100). Results demonstrated that weight-gain percentage (WG%) was not different between different sources of cottonseed meal (CCMP and LGCMP). However, values of WG% significantly differed among different replacement levels, with CCMP80 and LGCMP40 having significantly higher values compared to other treatments. Fish fed CCMP80 and LGCMP40 exhibited higher protein efficiency ratios (PERs) than fish fed other experimental diets. The regression analysis from a second-order or third-order polynomial model based on WG% showed that the optimal PBMP replacement levels by CCMP and LGCMP are 74% and 33%, respectively. The whole-body lipid contents remarkably decreased as dietary CCMP or LGCMP inclusion levels increased. The relative mRNA expression of insulin-like growth factor-1(IGF-1) in liver was higher in fish fed CCMP80 and LGCMP40 diets compared to fish fed other diets. Generally, in low-FM diets of hybrid grouper, CCMP and LGCMP could replace 74% and 33% of PBMP, respectively.https://www.mdpi.com/2076-2615/12/15/1906hybrid groupercottonseed mealgrowth performancefeed utilization |
| spellingShingle | Misbah Irm Bo Ye Xiaoyi Wu Lina Geng Qinxiao Cai Lu Zhang Haoyun Zhai Zhiyu Zhou Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity Animals hybrid grouper cottonseed meal growth performance feed utilization |
| title | Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity |
| title_full | Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity |
| title_fullStr | Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity |
| title_full_unstemmed | Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity |
| title_short | Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i><i>♀</i><i>× Epinephelus lanceolatus</i><i>♂</i>): Growth, Feed Utilization, Gut Histology, and Immunity |
| title_sort | assessment of conventional and low gossypol cottonseed meal as alternative protein sources in low fishmeal diets of hybrid grouper i epinephelus fuscoguttatus i i ♀ i i epinephelus lanceolatus i i ♂ i growth feed utilization gut histology and immunity |
| topic | hybrid grouper cottonseed meal growth performance feed utilization |
| url | https://www.mdpi.com/2076-2615/12/15/1906 |
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