Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation
Casuarina equisetifolia (C. equisetifolia) is an economically important forest tree species, often cultivated in continuous monoculture as a coastal protection forest. Continuous planting has gradually affected growth and severely restricted the sustainable development of the C. equisetifolia indust...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1324184/full |
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| author | Yuhua Wang Yuhua Wang Shaoxiong Lin Jianjuan Li Xiaoli Jia Xiaoli Jia Mingyue Hu Mingyue Hu Yuhong Cai Yuhong Cai Pengyuan Cheng Mingzhe Li Yiling Chen Wenxiong Lin Wenxiong Lin Haibin Wang Haibin Wang Zeyan Wu Zeyan Wu |
| author_facet | Yuhua Wang Yuhua Wang Shaoxiong Lin Jianjuan Li Xiaoli Jia Xiaoli Jia Mingyue Hu Mingyue Hu Yuhong Cai Yuhong Cai Pengyuan Cheng Mingzhe Li Yiling Chen Wenxiong Lin Wenxiong Lin Haibin Wang Haibin Wang Zeyan Wu Zeyan Wu |
| author_sort | Yuhua Wang |
| collection | DOAJ |
| description | Casuarina equisetifolia (C. equisetifolia) is an economically important forest tree species, often cultivated in continuous monoculture as a coastal protection forest. Continuous planting has gradually affected growth and severely restricted the sustainable development of the C. equisetifolia industry. In this study, we analyzed the effects of continuous planting on C. equisetifolia growth and explored the rhizosphere soil microecological mechanism from a metagenomic perspective. The results showed that continuous planting resulted in dwarfing, shorter root length, and reduced C. equisetifolia seedling root system. Metagenomics analysis showed that 10 key characteristic microorganisms, mainly Actinoallomurus, Actinomadura, and Mycobacterium, were responsible for continuously planted C. equisetifolia trees. Quantitative analysis showed that the number of microorganisms in these three genera decreased significantly with the increase of continuous planting. Gene function analysis showed that continuous planting led to the weakening of the environmental information processing-signal transduction ability of soil characteristic microorganisms, and the decrease of C. equisetifolia trees against stress. Reduced capacity for metabolism, genetic information processing-replication and repair resulted in reduced microbial propagation and reduced microbial quantity in the rhizosphere soil of C. equisetifolia trees. Secondly, amino acid metabolism, carbohydrate metabolism, glycan biosynthesis and metabolism, lipid metabolism, metabolism of cofactors and vitamins were all significantly reduced, resulting in a decrease in the ability of the soil to synthesize and metabolize carbon and nitrogen. These reduced capacities further led to reduced soil microbial quantity, microbial carbon and nitrogen, microbial respiration intensity, reduced soil enzyme nutrient cycling and resistance-related enzyme activities, a significant reduction in available nutrient content of rhizosphere soils, a reduction in the ion exchange capacity, and an impediment to C. equisetifolia growth. This study provides an important basis for the management of continuously planted C. equisetifolia plantations. |
| first_indexed | 2024-03-09T02:35:43Z |
| format | Article |
| id | doaj.art-ab41cc1cda6444be8dda8ef26c4f2388 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-09T02:35:43Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-ab41cc1cda6444be8dda8ef26c4f23882023-12-06T08:36:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-12-011410.3389/fpls.2023.13241841324184Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformationYuhua Wang0Yuhua Wang1Shaoxiong Lin2Jianjuan Li3Xiaoli Jia4Xiaoli Jia5Mingyue Hu6Mingyue Hu7Yuhong Cai8Yuhong Cai9Pengyuan Cheng10Mingzhe Li11Yiling Chen12Wenxiong Lin13Wenxiong Lin14Haibin Wang15Haibin Wang16Zeyan Wu17Zeyan Wu18College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Life Science, Longyan University, Longyan, ChinaEditorial Department, Fujian Academy of Forestry Survey and Planning, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Tea and Food, Wuyi University, Wuyishan, ChinaCollege of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Life Science, Longyan University, Longyan, ChinaCollege of Life Science, Longyan University, Longyan, ChinaCollege of Life Science, Longyan University, Longyan, ChinaCollege of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Tea and Food, Wuyi University, Wuyishan, ChinaCollege of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaCasuarina equisetifolia (C. equisetifolia) is an economically important forest tree species, often cultivated in continuous monoculture as a coastal protection forest. Continuous planting has gradually affected growth and severely restricted the sustainable development of the C. equisetifolia industry. In this study, we analyzed the effects of continuous planting on C. equisetifolia growth and explored the rhizosphere soil microecological mechanism from a metagenomic perspective. The results showed that continuous planting resulted in dwarfing, shorter root length, and reduced C. equisetifolia seedling root system. Metagenomics analysis showed that 10 key characteristic microorganisms, mainly Actinoallomurus, Actinomadura, and Mycobacterium, were responsible for continuously planted C. equisetifolia trees. Quantitative analysis showed that the number of microorganisms in these three genera decreased significantly with the increase of continuous planting. Gene function analysis showed that continuous planting led to the weakening of the environmental information processing-signal transduction ability of soil characteristic microorganisms, and the decrease of C. equisetifolia trees against stress. Reduced capacity for metabolism, genetic information processing-replication and repair resulted in reduced microbial propagation and reduced microbial quantity in the rhizosphere soil of C. equisetifolia trees. Secondly, amino acid metabolism, carbohydrate metabolism, glycan biosynthesis and metabolism, lipid metabolism, metabolism of cofactors and vitamins were all significantly reduced, resulting in a decrease in the ability of the soil to synthesize and metabolize carbon and nitrogen. These reduced capacities further led to reduced soil microbial quantity, microbial carbon and nitrogen, microbial respiration intensity, reduced soil enzyme nutrient cycling and resistance-related enzyme activities, a significant reduction in available nutrient content of rhizosphere soils, a reduction in the ion exchange capacity, and an impediment to C. equisetifolia growth. This study provides an important basis for the management of continuously planted C. equisetifolia plantations.https://www.frontiersin.org/articles/10.3389/fpls.2023.1324184/fullC. equisetifoliacontinuous plantingmetagenomenutrient transformationsoil enzymesgene function |
| spellingShingle | Yuhua Wang Yuhua Wang Shaoxiong Lin Jianjuan Li Xiaoli Jia Xiaoli Jia Mingyue Hu Mingyue Hu Yuhong Cai Yuhong Cai Pengyuan Cheng Mingzhe Li Yiling Chen Wenxiong Lin Wenxiong Lin Haibin Wang Haibin Wang Zeyan Wu Zeyan Wu Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation Frontiers in Plant Science C. equisetifolia continuous planting metagenome nutrient transformation soil enzymes gene function |
| title | Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| title_full | Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| title_fullStr | Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| title_full_unstemmed | Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| title_short | Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| title_sort | metagenomics based exploration of key soil microorganisms contributing to continuously planted casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation |
| topic | C. equisetifolia continuous planting metagenome nutrient transformation soil enzymes gene function |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1324184/full |
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