Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers
The red swamp crayfish (<i>Procambarus clarkii</i>) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally develo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/7/4/142 |
| _version_ | 1797410372634804224 |
|---|---|
| author | Xin-Fen Guo Min Liu Yu-Lin Zhou Wen-Yu Wei Zhi Li Li Zhou Zhong-Wei Wang Jian-Fang Gui |
| author_facet | Xin-Fen Guo Min Liu Yu-Lin Zhou Wen-Yu Wei Zhi Li Li Zhou Zhong-Wei Wang Jian-Fang Gui |
| author_sort | Xin-Fen Guo |
| collection | DOAJ |
| description | The red swamp crayfish (<i>Procambarus clarkii</i>) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish. |
| first_indexed | 2024-03-09T04:28:59Z |
| format | Article |
| id | doaj.art-ab42feb311cb4270924f68ffdbcc3575 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T04:28:59Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-ab42feb311cb4270924f68ffdbcc35752023-12-03T13:38:28ZengMDPI AGFishes2410-38882022-06-017414210.3390/fishes7040142Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR MarkersXin-Fen Guo0Min Liu1Yu-Lin Zhou2Wen-Yu Wei3Zhi Li4Li Zhou5Zhong-Wei Wang6Jian-Fang Gui7State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430061, ChinaThe red swamp crayfish (<i>Procambarus clarkii</i>) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish.https://www.mdpi.com/2410-3888/7/4/142<i>Procambarus clarkii</i>microsatellitegenetic diversitydifferentiation |
| spellingShingle | Xin-Fen Guo Min Liu Yu-Lin Zhou Wen-Yu Wei Zhi Li Li Zhou Zhong-Wei Wang Jian-Fang Gui Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers Fishes <i>Procambarus clarkii</i> microsatellite genetic diversity differentiation |
| title | Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers |
| title_full | Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers |
| title_fullStr | Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers |
| title_full_unstemmed | Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers |
| title_short | Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (<i>Procambarus clarkii</i>) from Lakes and Rice Fields by SSR Markers |
| title_sort | genetic diversity evaluation and population structure analysis of red swamp crayfish i procambarus clarkii i from lakes and rice fields by ssr markers |
| topic | <i>Procambarus clarkii</i> microsatellite genetic diversity differentiation |
| url | https://www.mdpi.com/2410-3888/7/4/142 |
| work_keys_str_mv | AT xinfenguo geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT minliu geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT yulinzhou geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT wenyuwei geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT zhili geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT lizhou geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT zhongweiwang geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers AT jianfanggui geneticdiversityevaluationandpopulationstructureanalysisofredswampcrayfishiprocambarusclarkiiifromlakesandricefieldsbyssrmarkers |