Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i>
<i>Streptomyces</i> is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mi...
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MDPI AG
2021-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/4/1851 |
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| author | Van Thuy Thi Pham Chung Thanh Nguyen Dipesh Dhakal Hue Thi Nguyen Tae-Su Kim Jae Kyung Sohng |
| author_facet | Van Thuy Thi Pham Chung Thanh Nguyen Dipesh Dhakal Hue Thi Nguyen Tae-Su Kim Jae Kyung Sohng |
| author_sort | Van Thuy Thi Pham |
| collection | DOAJ |
| description | <i>Streptomyces</i> is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches demonstrate that many silent and cryptic biosynthetic gene clusters (BGCs) and many secondary metabolites are produced in very low amounts under laboratory conditions. One strain many compounds (OSMAC), overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to activate or enhance the production titer of target compounds. Hence, the heterologous expression of BGCs by transferring to a suitable production platform has been successfully employed for the detection, characterization, and yield quantity production of many secondary metabolites. In this review, we introduce the systematic approach for the heterologous production of secondary metabolites from <i>Streptomyces</i> in <i>Streptomyces</i> and other hosts, the genome analysis tools, the host selection, and the development of genetic control elements for heterologous expression and the production of secondary metabolites. |
| first_indexed | 2024-03-09T00:43:58Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T00:43:58Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-ab46e056af4c4a9699a23e88c132af902023-12-11T17:41:42ZengMDPI AGApplied Sciences2076-34172021-02-01114185110.3390/app11041851Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i>Van Thuy Thi Pham0Chung Thanh Nguyen1Dipesh Dhakal2Hue Thi Nguyen3Tae-Su Kim4Jae Kyung Sohng5Department of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, KoreaDepartment of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, KoreaDepartment of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, KoreaDepartment of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, KoreaDepartment of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, KoreaDepartment of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Korea<i>Streptomyces</i> is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches demonstrate that many silent and cryptic biosynthetic gene clusters (BGCs) and many secondary metabolites are produced in very low amounts under laboratory conditions. One strain many compounds (OSMAC), overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to activate or enhance the production titer of target compounds. Hence, the heterologous expression of BGCs by transferring to a suitable production platform has been successfully employed for the detection, characterization, and yield quantity production of many secondary metabolites. In this review, we introduce the systematic approach for the heterologous production of secondary metabolites from <i>Streptomyces</i> in <i>Streptomyces</i> and other hosts, the genome analysis tools, the host selection, and the development of genetic control elements for heterologous expression and the production of secondary metabolites.https://www.mdpi.com/2076-3417/11/4/1851heterologous expression<i>Streptomyces</i>secondary metabolite |
| spellingShingle | Van Thuy Thi Pham Chung Thanh Nguyen Dipesh Dhakal Hue Thi Nguyen Tae-Su Kim Jae Kyung Sohng Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> Applied Sciences heterologous expression <i>Streptomyces</i> secondary metabolite |
| title | Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> |
| title_full | Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> |
| title_fullStr | Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> |
| title_full_unstemmed | Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> |
| title_short | Recent Advances in the Heterologous Biosynthesis of Natural Products from <i>Streptomyces</i> |
| title_sort | recent advances in the heterologous biosynthesis of natural products from i streptomyces i |
| topic | heterologous expression <i>Streptomyces</i> secondary metabolite |
| url | https://www.mdpi.com/2076-3417/11/4/1851 |
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