Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel

Incorporation of vegetable oils through pre-emulsification has received notable attention for delivering polyunsaturated fatty acids to emulsified-type meat products. The two important influencing factors of the rheological property of composite myofibrillar protein (MP) gel are emulsion droplet siz...

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Main Authors: Ruying Cai, Zongyun Yang, Zhen Li, Peng Wang, Minyi Han, Xinglian Xu
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/5/629
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author Ruying Cai
Zongyun Yang
Zhen Li
Peng Wang
Minyi Han
Xinglian Xu
author_facet Ruying Cai
Zongyun Yang
Zhen Li
Peng Wang
Minyi Han
Xinglian Xu
author_sort Ruying Cai
collection DOAJ
description Incorporation of vegetable oils through pre-emulsification has received notable attention for delivering polyunsaturated fatty acids to emulsified-type meat products. The two important influencing factors of the rheological property of composite myofibrillar protein (MP) gel are emulsion droplet size and active or inactive interaction between interface and meat proteins. Incorporation of nonmeat protein emulsion (2% protein (<i>w</i>/<i>w</i>), egg-white protein isolate (EPI), porcine plasma protein (PPP), or sodium caseinate (SC)) with different droplet sizes (nano or macro) to a model of 2% MP gel was investigated in this research. The results of drop size measurement showed that 15,000 psi homogenizing could decrease the diameter of emulsion drop from macro- to nanoscale in the range of 324.4–734.5 nm. Active fillers (PPP and EPI emulsions) with nanodroplet size did not influence the viscosity of emulsion-filled composite cold sols but caused positive filling effects on the MP gel matrix after heating, as evidenced by the density microstructure. PPP and EPI nano-emulsion-filled composite MP had a significant high storage modulus enforcement effect, which reached nearly eight times those of other treatments (<i>p</i> < 0.05). Similarly, the results of thermal scanning rheology and a large-deformation mechanical test showed that PPP and EPI emulsions with nanoscale droplets, other than macroscale, had the highest gel strength of heat-induced emulsion-filled composite MP gel (<i>p</i> < 0.05). Overall, these findings will be helpful for selecting the correct pre-emulsified protein and designing the textural properties of foods.
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spelling doaj.art-ab4758c6c1cb432e86671e9ebb4af33f2023-11-23T23:00:02ZengMDPI AGFoods2304-81582022-02-0111562910.3390/foods11050629Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein GelRuying Cai0Zongyun Yang1Zhen Li2Peng Wang3Minyi Han4Xinglian Xu5Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaIncorporation of vegetable oils through pre-emulsification has received notable attention for delivering polyunsaturated fatty acids to emulsified-type meat products. The two important influencing factors of the rheological property of composite myofibrillar protein (MP) gel are emulsion droplet size and active or inactive interaction between interface and meat proteins. Incorporation of nonmeat protein emulsion (2% protein (<i>w</i>/<i>w</i>), egg-white protein isolate (EPI), porcine plasma protein (PPP), or sodium caseinate (SC)) with different droplet sizes (nano or macro) to a model of 2% MP gel was investigated in this research. The results of drop size measurement showed that 15,000 psi homogenizing could decrease the diameter of emulsion drop from macro- to nanoscale in the range of 324.4–734.5 nm. Active fillers (PPP and EPI emulsions) with nanodroplet size did not influence the viscosity of emulsion-filled composite cold sols but caused positive filling effects on the MP gel matrix after heating, as evidenced by the density microstructure. PPP and EPI nano-emulsion-filled composite MP had a significant high storage modulus enforcement effect, which reached nearly eight times those of other treatments (<i>p</i> < 0.05). Similarly, the results of thermal scanning rheology and a large-deformation mechanical test showed that PPP and EPI emulsions with nanoscale droplets, other than macroscale, had the highest gel strength of heat-induced emulsion-filled composite MP gel (<i>p</i> < 0.05). Overall, these findings will be helpful for selecting the correct pre-emulsified protein and designing the textural properties of foods.https://www.mdpi.com/2304-8158/11/5/629myofibrillar proteinnano-emulsionrheological propertyactive interaction
spellingShingle Ruying Cai
Zongyun Yang
Zhen Li
Peng Wang
Minyi Han
Xinglian Xu
Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
Foods
myofibrillar protein
nano-emulsion
rheological property
active interaction
title Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
title_full Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
title_fullStr Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
title_full_unstemmed Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
title_short Nano Filling Effect of Nonmeat Protein Emulsion on the Rheological Property of Myofibrillar Protein Gel
title_sort nano filling effect of nonmeat protein emulsion on the rheological property of myofibrillar protein gel
topic myofibrillar protein
nano-emulsion
rheological property
active interaction
url https://www.mdpi.com/2304-8158/11/5/629
work_keys_str_mv AT ruyingcai nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel
AT zongyunyang nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel
AT zhenli nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel
AT pengwang nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel
AT minyihan nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel
AT xinglianxu nanofillingeffectofnonmeatproteinemulsionontherheologicalpropertyofmyofibrillarproteingel