A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs
Male guinea pigs show high susceptibility to Burkholderia mallei and have been used as animal models in glanders studies. The purpose of our study was to elucidate glanders comparative pathogenesis in guinea pigs. We present here the histological changes and bacterial isolation that develop over tim...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2015-12-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://www.archrazi.com/browse.php?a_id=600&sid=1&slc_lang=en |
| _version_ | 1811260933757992960 |
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| author | Eslampanah, M. Hablolvarid, M.H. Mosavari, N. |
| author_facet | Eslampanah, M. Hablolvarid, M.H. Mosavari, N. |
| author_sort | Eslampanah, M. |
| collection | DOAJ |
| description | Male guinea pigs show high susceptibility to Burkholderia mallei and have been used as animal models in glanders studies. The purpose of our study was to elucidate glanders comparative pathogenesis in guinea pigs. We present here the histological changes and bacterial isolation that develop over time in guinea pigs inoculated intraperitoneally (IP) with two strain of B. mallei. Ten male guinea pigs were inoculated intraperitoneally with either the standard strain of Burkholderia mallei or B. mallei strain from Siberian tiger at the Tehran zoo individually, then euthanized at multiple time points post inoculation. Histopathologic changes were similar in both groups and consisted of pyogranulomatous inflammation. In the standard strain study guinea pigs, changes were first seen at 48 hours in liver and heart then in spleen, lung, and kidney at day 3. These changes generally reached maximal incidence and severity by day 3 but decreased by comparison in all tissues except the liver, lung and kidney. Changes were first seen in Siberian tiger strain study guinea pigs also at 48 hours in lung, liver and spleen. At day 3, changes were present in liver, spleen and mediastinal lymph nodes. These changes were maximal at day 4 and 5. In contrast there are differences in incidence and severity between the two strain study guinea pigs. Our findings based on histopathological study indicate that Siberian tiger strain has more severity in gross and necropsy examination but in pathologic lesion was qualitatively similar generally. Additionally, by bacterial isolation, we confirmed the presence of B. mallei. |
| first_indexed | 2024-04-12T18:54:58Z |
| format | Article |
| id | doaj.art-ab4808f34f594ab19a77d1bc0397bea9 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-12T18:54:58Z |
| publishDate | 2015-12-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-ab4808f34f594ab19a77d1bc0397bea92022-12-22T03:20:21ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722015-12-01704245254A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigsEslampanah, M.0Hablolvarid, M.H.1Mosavari, N.2Department of Pathology, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of Pathology, Razi Vaccine and Serum Research Institute, Karaj, IranDepartment of PPD Production, Razi Vaccine and Serum Research Institute, Karaj, IranMale guinea pigs show high susceptibility to Burkholderia mallei and have been used as animal models in glanders studies. The purpose of our study was to elucidate glanders comparative pathogenesis in guinea pigs. We present here the histological changes and bacterial isolation that develop over time in guinea pigs inoculated intraperitoneally (IP) with two strain of B. mallei. Ten male guinea pigs were inoculated intraperitoneally with either the standard strain of Burkholderia mallei or B. mallei strain from Siberian tiger at the Tehran zoo individually, then euthanized at multiple time points post inoculation. Histopathologic changes were similar in both groups and consisted of pyogranulomatous inflammation. In the standard strain study guinea pigs, changes were first seen at 48 hours in liver and heart then in spleen, lung, and kidney at day 3. These changes generally reached maximal incidence and severity by day 3 but decreased by comparison in all tissues except the liver, lung and kidney. Changes were first seen in Siberian tiger strain study guinea pigs also at 48 hours in lung, liver and spleen. At day 3, changes were present in liver, spleen and mediastinal lymph nodes. These changes were maximal at day 4 and 5. In contrast there are differences in incidence and severity between the two strain study guinea pigs. Our findings based on histopathological study indicate that Siberian tiger strain has more severity in gross and necropsy examination but in pathologic lesion was qualitatively similar generally. Additionally, by bacterial isolation, we confirmed the presence of B. mallei.http://www.archrazi.com/browse.php?a_id=600&sid=1&slc_lang=enBurkholderia malleiSiberian tiger strainstandard strainpathologic lesion |
| spellingShingle | Eslampanah, M. Hablolvarid, M.H. Mosavari, N. A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs Archives of Razi Institute Burkholderia mallei Siberian tiger strain standard strain pathologic lesion |
| title | A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs |
| title_full | A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs |
| title_fullStr | A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs |
| title_full_unstemmed | A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs |
| title_short | A comparison of virulence of intraperitoneal infection of Burkholderia mallei strains in guinea-pigs |
| title_sort | comparison of virulence of intraperitoneal infection of burkholderia mallei strains in guinea pigs |
| topic | Burkholderia mallei Siberian tiger strain standard strain pathologic lesion |
| url | http://www.archrazi.com/browse.php?a_id=600&sid=1&slc_lang=en |
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