In vivo expression of peptidylarginine deiminase in Drosophila melanogaster.
Peptidylarginine deiminase (PAD) modifies peptidylarginine and converts it to peptidylcitrulline in the presence of elevated calcium. Protein modification can lead to severe changes in protein structure and function, and aberrant PAD activity is linked to human pathologies. While PAD homologs have b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0227822 |
| _version_ | 1818728422644383744 |
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| author | Olena Mahneva Monica G Risley Ciny John Sarah L Milton Ken Dawson-Scully William W Ja |
| author_facet | Olena Mahneva Monica G Risley Ciny John Sarah L Milton Ken Dawson-Scully William W Ja |
| author_sort | Olena Mahneva |
| collection | DOAJ |
| description | Peptidylarginine deiminase (PAD) modifies peptidylarginine and converts it to peptidylcitrulline in the presence of elevated calcium. Protein modification can lead to severe changes in protein structure and function, and aberrant PAD activity is linked to human pathologies. While PAD homologs have been discovered in vertebrates-as well as in protozoa, fungi, and bacteria-none have been identified in Drosophila melanogaster, a simple and widely used animal model for human diseases. Here, we describe the development of a human PAD overexpression model in Drosophila. We established fly lines harboring human PAD2 or PAD4 transgenes for ectopic expression under control of the GAL4/UAS system. We show that ubiquitous or nervous system expression of PAD2 or PAD4 have minimal impact on fly lifespan, fecundity, and the response to acute heat stress. Although we did not detect citrullinated proteins in fly homogenates, fly-expressed PAD4-but not PAD2-was active in vitro upon Ca2+ supplementation. The transgenic fly lines may be valuable in future efforts to develop animal models of PAD-related disorders and for investigating the biochemistry and regulation of PAD function. |
| first_indexed | 2024-12-17T22:29:45Z |
| format | Article |
| id | doaj.art-ab498490cadb496494ae559d021785b4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:29:45Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ab498490cadb496494ae559d021785b42022-12-21T21:30:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022782210.1371/journal.pone.0227822In vivo expression of peptidylarginine deiminase in Drosophila melanogaster.Olena MahnevaMonica G RisleyCiny JohnSarah L MiltonKen Dawson-ScullyWilliam W JaPeptidylarginine deiminase (PAD) modifies peptidylarginine and converts it to peptidylcitrulline in the presence of elevated calcium. Protein modification can lead to severe changes in protein structure and function, and aberrant PAD activity is linked to human pathologies. While PAD homologs have been discovered in vertebrates-as well as in protozoa, fungi, and bacteria-none have been identified in Drosophila melanogaster, a simple and widely used animal model for human diseases. Here, we describe the development of a human PAD overexpression model in Drosophila. We established fly lines harboring human PAD2 or PAD4 transgenes for ectopic expression under control of the GAL4/UAS system. We show that ubiquitous or nervous system expression of PAD2 or PAD4 have minimal impact on fly lifespan, fecundity, and the response to acute heat stress. Although we did not detect citrullinated proteins in fly homogenates, fly-expressed PAD4-but not PAD2-was active in vitro upon Ca2+ supplementation. The transgenic fly lines may be valuable in future efforts to develop animal models of PAD-related disorders and for investigating the biochemistry and regulation of PAD function.https://doi.org/10.1371/journal.pone.0227822 |
| spellingShingle | Olena Mahneva Monica G Risley Ciny John Sarah L Milton Ken Dawson-Scully William W Ja In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. PLoS ONE |
| title | In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. |
| title_full | In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. |
| title_fullStr | In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. |
| title_full_unstemmed | In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. |
| title_short | In vivo expression of peptidylarginine deiminase in Drosophila melanogaster. |
| title_sort | in vivo expression of peptidylarginine deiminase in drosophila melanogaster |
| url | https://doi.org/10.1371/journal.pone.0227822 |
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