Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis
Abstract Background The increase in drug-resistant opportunistic pathogenic bacteria, especially of antibiotic-resistant Staphylococcus epidermidis (S. epidermidis), has led to difficulties in the treatment of skin and soft tissue infections (SSTI). The major reason for bacterial resistance is the f...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02578-y |
| _version_ | 1818474016084590592 |
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| author | Zhiling Cai Zitong Mo Shiqian Zheng Shihua Lan Shengjun Xie Jinghui Lu Chunping Tang Zhibin Shen |
| author_facet | Zhiling Cai Zitong Mo Shiqian Zheng Shihua Lan Shengjun Xie Jinghui Lu Chunping Tang Zhibin Shen |
| author_sort | Zhiling Cai |
| collection | DOAJ |
| description | Abstract Background The increase in drug-resistant opportunistic pathogenic bacteria, especially of antibiotic-resistant Staphylococcus epidermidis (S. epidermidis), has led to difficulties in the treatment of skin and soft tissue infections (SSTI). The major reason for bacterial resistance is the formation of bacterial biofilm. Here, we report a promising combination therapy of flavaspidic acid BB (BB) and mupirocin, which can effectively eradicate the biofilm of S. epidermidis and eliminate its drug resistance. Result The susceptibility test showed that the combination of BB and mupirocin has good antibacterial and antibiofilm activities, and the fractional inhibitory concentration index (FICI) of BB combined with mupirocin was 0.51 ± 0.00 ~ 0.75 ± 0.05, showing synergistic effect. Moreover, the time-kill curve assay results indicated that the combination of drugs can effectively inhibit the planktonic S. epidermidis. After drugs treatment, the drug-combination showed significantly inhibitory effects on the metabolic activity and total biomass in each stage of biofilm formation. The synergistic effect is likely related to the adhesion between bacteria, which is confirmed by field emission scanning electron microscope. And the expression level of aap, sarA and agrA genes were detected by real-time quantitative PCR (qRT-PCR). Conclusion Our study provides the experimental data for the use of BB for the clinical treatment of skin infections and further demonstrate the potential of BB as a novel biofilm inhibitor. |
| first_indexed | 2024-04-14T04:30:50Z |
| format | Article |
| id | doaj.art-ab4abd3af0cd4c7fb973816c9c7e1d36 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-14T04:30:50Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ab4abd3af0cd4c7fb973816c9c7e1d362022-12-22T02:12:04ZengBMCBMC Microbiology1471-21802022-07-0122111310.1186/s12866-022-02578-yFlavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidisZhiling Cai0Zitong Mo1Shiqian Zheng2Shihua Lan3Shengjun Xie4Jinghui Lu5Chunping Tang6Zhibin Shen7School of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversityThe First Affiliated Hospital of Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Guangdong Pharmaceutical UniversityAbstract Background The increase in drug-resistant opportunistic pathogenic bacteria, especially of antibiotic-resistant Staphylococcus epidermidis (S. epidermidis), has led to difficulties in the treatment of skin and soft tissue infections (SSTI). The major reason for bacterial resistance is the formation of bacterial biofilm. Here, we report a promising combination therapy of flavaspidic acid BB (BB) and mupirocin, which can effectively eradicate the biofilm of S. epidermidis and eliminate its drug resistance. Result The susceptibility test showed that the combination of BB and mupirocin has good antibacterial and antibiofilm activities, and the fractional inhibitory concentration index (FICI) of BB combined with mupirocin was 0.51 ± 0.00 ~ 0.75 ± 0.05, showing synergistic effect. Moreover, the time-kill curve assay results indicated that the combination of drugs can effectively inhibit the planktonic S. epidermidis. After drugs treatment, the drug-combination showed significantly inhibitory effects on the metabolic activity and total biomass in each stage of biofilm formation. The synergistic effect is likely related to the adhesion between bacteria, which is confirmed by field emission scanning electron microscope. And the expression level of aap, sarA and agrA genes were detected by real-time quantitative PCR (qRT-PCR). Conclusion Our study provides the experimental data for the use of BB for the clinical treatment of skin infections and further demonstrate the potential of BB as a novel biofilm inhibitor.https://doi.org/10.1186/s12866-022-02578-yCombination therapyFlavaspidic acid BBBiofilmStaphylococcus epidermidis |
| spellingShingle | Zhiling Cai Zitong Mo Shiqian Zheng Shihua Lan Shengjun Xie Jinghui Lu Chunping Tang Zhibin Shen Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis BMC Microbiology Combination therapy Flavaspidic acid BB Biofilm Staphylococcus epidermidis |
| title | Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis |
| title_full | Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis |
| title_fullStr | Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis |
| title_full_unstemmed | Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis |
| title_short | Flavaspidic acid BB combined with mupirocin improves its anti-bacterial and anti-biofilm activities against Staphylococcus epidermidis |
| title_sort | flavaspidic acid bb combined with mupirocin improves its anti bacterial and anti biofilm activities against staphylococcus epidermidis |
| topic | Combination therapy Flavaspidic acid BB Biofilm Staphylococcus epidermidis |
| url | https://doi.org/10.1186/s12866-022-02578-y |
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