Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (...
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/9/2157 |
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| author | Sucheewin Krobthong Yodying Yingchutrakul Wonnop Visessanguan Thanisorn Mahatnirunkul Pawitrabhorn Samutrtai Chartchai Chaichana Phakorn Papan Kiattawee Choowongkomon |
| author_facet | Sucheewin Krobthong Yodying Yingchutrakul Wonnop Visessanguan Thanisorn Mahatnirunkul Pawitrabhorn Samutrtai Chartchai Chaichana Phakorn Papan Kiattawee Choowongkomon |
| author_sort | Sucheewin Krobthong |
| collection | DOAJ |
| description | Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X<sub>1</sub>) and enzyme usage (X<sub>2</sub>) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X<sub>1</sub> at 8.63 h and X<sub>2</sub> at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity |
| first_indexed | 2024-03-10T07:40:53Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T07:40:53Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ab4caa1a86c045bf98866e20873170262023-11-22T13:05:06ZengMDPI AGFoods2304-81582021-09-01109215710.3390/foods10092157Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics ApproachSucheewin Krobthong0Yodying Yingchutrakul1Wonnop Visessanguan2Thanisorn Mahatnirunkul3Pawitrabhorn Samutrtai4Chartchai Chaichana5Phakorn Papan6Kiattawee Choowongkomon7Interdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok 10900, ThailandNational Omics Center, NSTDA, Pathum Thani 12120, ThailandNational Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani 12120, ThailandNational Nanotechnology Center, NSTDA, Pathum Thani 12120, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandSiriraj Center of Research Excellence for Diabetes and Obesity (SiCORE-DO), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandInterdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok 10900, ThailandExcessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X<sub>1</sub>) and enzyme usage (X<sub>2</sub>) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X<sub>1</sub> at 8.63 h and X<sub>2</sub> at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesityhttps://www.mdpi.com/2304-8158/10/9/2157RSMLingzhihypolipidemic activitypeptides3T3-L1 |
| spellingShingle | Sucheewin Krobthong Yodying Yingchutrakul Wonnop Visessanguan Thanisorn Mahatnirunkul Pawitrabhorn Samutrtai Chartchai Chaichana Phakorn Papan Kiattawee Choowongkomon Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach Foods RSM Lingzhi hypolipidemic activity peptides 3T3-L1 |
| title | Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
| title_full | Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
| title_fullStr | Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
| title_full_unstemmed | Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
| title_short | Study of the Lipolysis Effect of Nanoliposome-Encapsulated <i>Ganoderma lucidum</i> Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
| title_sort | study of the lipolysis effect of nanoliposome encapsulated i ganoderma lucidum i protein hydrolysates on adipocyte cells using proteomics approach |
| topic | RSM Lingzhi hypolipidemic activity peptides 3T3-L1 |
| url | https://www.mdpi.com/2304-8158/10/9/2157 |
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