<it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1
<p>Abstract</p> <p>Background</p> <p>In <it>Xenopus </it>retinogenesis, p27Xic1, a <it>Xenopus </it>cyclin dependent kinase inhibitor, functions as a cell fate determinant in both gliogenesis and neurogenesis in a context dependent manner. This a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-01-01
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| Series: | Neural Development |
| Online Access: | http://www.neuraldevelopment.com/content/4/1/1 |
| _version_ | 1811284861871194112 |
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| author | Zollo Massimo Hussain Kamran Waters Caroline T Bilitou Aikaterini Mochizuki Toshiaki Ohnuma Shin-ichi |
| author_facet | Zollo Massimo Hussain Kamran Waters Caroline T Bilitou Aikaterini Mochizuki Toshiaki Ohnuma Shin-ichi |
| author_sort | Zollo Massimo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In <it>Xenopus </it>retinogenesis, p27Xic1, a <it>Xenopus </it>cyclin dependent kinase inhibitor, functions as a cell fate determinant in both gliogenesis and neurogenesis in a context dependent manner. This activity is essential for co-ordination of determination and cell cycle regulation. However, very little is known about the mechanism regulating the context dependent choice between gliogenesis versus neurogenesis.</p> <p>Results</p> <p>We have identified NM23-X4, a NM23 family member, as a binding partner of p27Xic1. NM23-X4 is expressed at the periphery of the ciliary marginal zone of the <it>Xenopus </it>retina and the expression overlaps with p27Xic1 at the central side. Our <it>in vivo </it>functional analysis in <it>Xenopus </it>retina has shown that knockdown of NM23-X4 activates gliogenesis. Furthermore, co-overexpression of NM23-X4 with p27Xic1 results in the inhibition of p27Xic1-mediated gliogenesis, through direct interaction of NM23-X4 with the amino-terminal side of p27Xic1. This inhibitory effect on gliogenesis requires serine-150 and histidine-148, which correspond to the important residues for the kinase activities of NM23 family members.</p> <p>Conclusion</p> <p>This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1-mediated gliogenesis in <it>Xenopus </it>retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenesis.</p> |
| first_indexed | 2024-04-13T02:35:57Z |
| format | Article |
| id | doaj.art-ab4ece22194c4a12b92de08406943da0 |
| institution | Directory Open Access Journal |
| issn | 1749-8104 |
| language | English |
| last_indexed | 2024-04-13T02:35:57Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Neural Development |
| spelling | doaj.art-ab4ece22194c4a12b92de08406943da02022-12-22T03:06:22ZengBMCNeural Development1749-81042009-01-0141110.1186/1749-8104-4-1<it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1Zollo MassimoHussain KamranWaters Caroline TBilitou AikateriniMochizuki ToshiakiOhnuma Shin-ichi<p>Abstract</p> <p>Background</p> <p>In <it>Xenopus </it>retinogenesis, p27Xic1, a <it>Xenopus </it>cyclin dependent kinase inhibitor, functions as a cell fate determinant in both gliogenesis and neurogenesis in a context dependent manner. This activity is essential for co-ordination of determination and cell cycle regulation. However, very little is known about the mechanism regulating the context dependent choice between gliogenesis versus neurogenesis.</p> <p>Results</p> <p>We have identified NM23-X4, a NM23 family member, as a binding partner of p27Xic1. NM23-X4 is expressed at the periphery of the ciliary marginal zone of the <it>Xenopus </it>retina and the expression overlaps with p27Xic1 at the central side. Our <it>in vivo </it>functional analysis in <it>Xenopus </it>retina has shown that knockdown of NM23-X4 activates gliogenesis. Furthermore, co-overexpression of NM23-X4 with p27Xic1 results in the inhibition of p27Xic1-mediated gliogenesis, through direct interaction of NM23-X4 with the amino-terminal side of p27Xic1. This inhibitory effect on gliogenesis requires serine-150 and histidine-148, which correspond to the important residues for the kinase activities of NM23 family members.</p> <p>Conclusion</p> <p>This study demonstrates that NM23-X4 functions as an inhibitor of p27Xic1-mediated gliogenesis in <it>Xenopus </it>retina and suggests that this activity contributes to the proper spatio-temporal regulation of gliogenesis.</p>http://www.neuraldevelopment.com/content/4/1/1 |
| spellingShingle | Zollo Massimo Hussain Kamran Waters Caroline T Bilitou Aikaterini Mochizuki Toshiaki Ohnuma Shin-ichi <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 Neural Development |
| title | <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 |
| title_full | <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 |
| title_fullStr | <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 |
| title_full_unstemmed | <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 |
| title_short | <it>Xenopus </it>NM23-X4 regulates retinal gliogenesis through interaction with p27Xic1 |
| title_sort | it xenopus it nm23 x4 regulates retinal gliogenesis through interaction with p27xic1 |
| url | http://www.neuraldevelopment.com/content/4/1/1 |
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