Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy
Nanodiamonds have emerged as promising agents for sensing and imaging due to their exceptional photostability and sensitivity to the local nanoscale environment. Here, we introduce a hybrid system composed of a nanodiamond containing nitrogen-vacancy center that is paired to a gold nanoparticle via...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/3/148 |
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| author | Lukas Schmidheini Raphael F. Tiefenauer Volker Gatterdam Andreas Frutiger Takumi Sannomiya Morteza Aramesh |
| author_facet | Lukas Schmidheini Raphael F. Tiefenauer Volker Gatterdam Andreas Frutiger Takumi Sannomiya Morteza Aramesh |
| author_sort | Lukas Schmidheini |
| collection | DOAJ |
| description | Nanodiamonds have emerged as promising agents for sensing and imaging due to their exceptional photostability and sensitivity to the local nanoscale environment. Here, we introduce a hybrid system composed of a nanodiamond containing nitrogen-vacancy center that is paired to a gold nanoparticle via DNA hybridization. Using multiphoton optical studies, we demonstrate that the harmonic mode emission generated in gold nanoparticles induces a coupled fluorescence emission in nanodiamonds. We show that the flickering of harmonic emission in gold nanoparticles directly influences the nanodiamonds’ emissions, resulting in stochastic blinking. By utilizing the stochastic emission fluctuations, we present a proof-of-principle experiment to demonstrate the potential application of the hybrid system for super-resolution microscopy. The introduced system may find applications in intracellular biosensing and bioimaging due to the DNA-based coupling mechanism and also the attractive characteristics of harmonic generation, such as low power, low background and tissue transparency. |
| first_indexed | 2024-03-09T20:04:04Z |
| format | Article |
| id | doaj.art-ab53d52589b84e2ba1fb40cd74fc0739 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:04:04Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ab53d52589b84e2ba1fb40cd74fc07392023-11-24T00:36:17ZengMDPI AGBiosensors2079-63742022-02-0112314810.3390/bios12030148Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for NanoscopyLukas Schmidheini0Raphael F. Tiefenauer1Volker Gatterdam2Andreas Frutiger3Takumi Sannomiya4Morteza Aramesh5Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, SwitzerlandDepartment of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Yokohama 226-8503, JapanLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, SwitzerlandNanodiamonds have emerged as promising agents for sensing and imaging due to their exceptional photostability and sensitivity to the local nanoscale environment. Here, we introduce a hybrid system composed of a nanodiamond containing nitrogen-vacancy center that is paired to a gold nanoparticle via DNA hybridization. Using multiphoton optical studies, we demonstrate that the harmonic mode emission generated in gold nanoparticles induces a coupled fluorescence emission in nanodiamonds. We show that the flickering of harmonic emission in gold nanoparticles directly influences the nanodiamonds’ emissions, resulting in stochastic blinking. By utilizing the stochastic emission fluctuations, we present a proof-of-principle experiment to demonstrate the potential application of the hybrid system for super-resolution microscopy. The introduced system may find applications in intracellular biosensing and bioimaging due to the DNA-based coupling mechanism and also the attractive characteristics of harmonic generation, such as low power, low background and tissue transparency.https://www.mdpi.com/2079-6374/12/3/148blinking nanodiamondsgold nanoparticlesplasmonic couplingmultiphoton excitationnanoscopy |
| spellingShingle | Lukas Schmidheini Raphael F. Tiefenauer Volker Gatterdam Andreas Frutiger Takumi Sannomiya Morteza Aramesh Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy Biosensors blinking nanodiamonds gold nanoparticles plasmonic coupling multiphoton excitation nanoscopy |
| title | Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy |
| title_full | Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy |
| title_fullStr | Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy |
| title_full_unstemmed | Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy |
| title_short | Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy |
| title_sort | self assembly of nanodiamonds and plasmonic nanoparticles for nanoscopy |
| topic | blinking nanodiamonds gold nanoparticles plasmonic coupling multiphoton excitation nanoscopy |
| url | https://www.mdpi.com/2079-6374/12/3/148 |
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