Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides
Phoma stem canker, caused by Leptosphaeria spp. is generally managed with demethylation inhibitor (DMI) fungicides in the Czech Republic. However, there have been reports of resistance to DMIs in L. maculans populations. Fungicide resistance management recommends the application of fungicides with d...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-12-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202204-0004_evaluation-of-the-sensitivity-of-leptosphaeria-maculans-isolates-causing-phoma-stem-canker-in-oilseed-rape-in-t.php |
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| author | Olufadekemi Fajemisin Jana Mazáková Pavel Ryšánek |
| author_facet | Olufadekemi Fajemisin Jana Mazáková Pavel Ryšánek |
| author_sort | Olufadekemi Fajemisin |
| collection | DOAJ |
| description | Phoma stem canker, caused by Leptosphaeria spp. is generally managed with demethylation inhibitor (DMI) fungicides in the Czech Republic. However, there have been reports of resistance to DMIs in L. maculans populations. Fungicide resistance management recommends the application of fungicides with different modes of action, either as mixtures or in rotation with other fungicide classes. The objective, therefore, was to evaluate the efficacy of boscalid, dimoxystrobin and a mixture of boscalid and dimoxystrobin, belonging to the succinate dehydrogenase inhibitor and quinone outside inhibitor fungicide groups, respectively, on L. maculans isolates. A total of 41 and 285 isolates were tested using the mycelial growth and microtitre plate assays, respectively. The EC50 values for the mycelial growth plate method ranged from 0.026-0.984 µg/mL and 0.097-1.653 µg/mL for boscalid and boscalid + dimoxystrobin, respectively. For dimoxystrobin, the EC50 range was wide, between 0.053-95.59 µg/mL. The EC50 values for the microtitre plate assay ranged from 0.001 496-0.836 3 µg/mL and 7.3 × 10-4-2.801 µg/mL for boscalid and dimoxystrobin, respectively. The results also showed that the L. maculans conidia were more sensitive to boscalid and dimoxystrobin than mycelia. |
| first_indexed | 2024-04-10T08:14:23Z |
| format | Article |
| id | doaj.art-ab580eca7e764f6ca3cfb16599d5020e |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:14:23Z |
| publishDate | 2022-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-ab580eca7e764f6ca3cfb16599d5020e2023-02-23T03:44:38ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412022-12-0158430531410.17221/177/2021-PPSpps-202204-0004Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicidesOlufadekemi FajemisinJana Mazáková0Pavel Ryšánek1Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicPhoma stem canker, caused by Leptosphaeria spp. is generally managed with demethylation inhibitor (DMI) fungicides in the Czech Republic. However, there have been reports of resistance to DMIs in L. maculans populations. Fungicide resistance management recommends the application of fungicides with different modes of action, either as mixtures or in rotation with other fungicide classes. The objective, therefore, was to evaluate the efficacy of boscalid, dimoxystrobin and a mixture of boscalid and dimoxystrobin, belonging to the succinate dehydrogenase inhibitor and quinone outside inhibitor fungicide groups, respectively, on L. maculans isolates. A total of 41 and 285 isolates were tested using the mycelial growth and microtitre plate assays, respectively. The EC50 values for the mycelial growth plate method ranged from 0.026-0.984 µg/mL and 0.097-1.653 µg/mL for boscalid and boscalid + dimoxystrobin, respectively. For dimoxystrobin, the EC50 range was wide, between 0.053-95.59 µg/mL. The EC50 values for the microtitre plate assay ranged from 0.001 496-0.836 3 µg/mL and 7.3 × 10-4-2.801 µg/mL for boscalid and dimoxystrobin, respectively. The results also showed that the L. maculans conidia were more sensitive to boscalid and dimoxystrobin than mycelia.https://pps.agriculturejournals.cz/artkey/pps-202204-0004_evaluation-of-the-sensitivity-of-leptosphaeria-maculans-isolates-causing-phoma-stem-canker-in-oilseed-rape-in-t.phpfungicide resistanceblackleg diseasedmisdhiqoi |
| spellingShingle | Olufadekemi Fajemisin Jana Mazáková Pavel Ryšánek Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides Plant Protection Science fungicide resistance blackleg disease dmi sdhi qoi |
| title | Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides |
| title_full | Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides |
| title_fullStr | Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides |
| title_full_unstemmed | Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides |
| title_short | Evaluation of the sensitivity of Leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the Czech Republic to boscalid and dimoxystrobin fungicides |
| title_sort | evaluation of the sensitivity of leptosphaeria maculans isolates causing phoma stem canker in oilseed rape in the czech republic to boscalid and dimoxystrobin fungicides |
| topic | fungicide resistance blackleg disease dmi sdhi qoi |
| url | https://pps.agriculturejournals.cz/artkey/pps-202204-0004_evaluation-of-the-sensitivity-of-leptosphaeria-maculans-isolates-causing-phoma-stem-canker-in-oilseed-rape-in-t.php |
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