Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major
OBJECTIVE: The main objectives of this study are to investigate the prevalence of HCV among patients with β-thalassemia major and to determine the most prevalent genotype for this virus among them. METHODS: One hundred twenty-two β-thalassemia major patients who were previously dia...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2011-03-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-65872 |
| _version_ | 1797921028670750720 |
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| author | Suleimman Ahmad Al-Sweedan Said Jaradat Khitam Amer Wail Hayajneh Hazem Haddad |
| author_facet | Suleimman Ahmad Al-Sweedan Said Jaradat Khitam Amer Wail Hayajneh Hazem Haddad |
| author_sort | Suleimman Ahmad Al-Sweedan |
| collection | DOAJ |
| description | OBJECTIVE: The main objectives of this study are to investigate the prevalence of HCV among patients with β-thalassemia major and to determine the most prevalent genotype for this virus among them. METHODS: One hundred twenty-two β-thalassemia major patients who were previously diagnosed at the molecular level were included. All plasma samples were tested for the presence of antibodies by ELISA. Real-time polymerase chain reaction (PCR) was used in the quantitation the HCV RNA viral loads, and consequently, patients with high virus titer were genotyped by the linear array. RESULTS: Forty of the patients were anti-HCV positive. The prevalence of anti-HCV was significantly higher in patients who received blood transfusion before 1993 (83.7%) than in those who received it after 1993 (16.3%) (p=0.000). β-thalassemia major patients with HCV infection had significantly higher rates of elevated aspartate aminotransferase (54.4% vs 40.5%, p=0.045) and alanine aminotransferase (72.47% vs 37.47%, p=0.00) and of splenectomy (54.8% vs 45.2%, p=0.004) than β-thalassemia major patients without HCV. CONCLUSION: HCV genotype 4 is the commonest genotype in multi-transfused patients with β-thalassemia major in Jordan. |
| first_indexed | 2024-04-10T14:10:11Z |
| format | Article |
| id | doaj.art-ab5815495ff84e5d92f9498d72b290f7 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T14:10:11Z |
| publishDate | 2011-03-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-ab5815495ff84e5d92f9498d72b290f72023-02-15T16:09:48ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632011-03-01281475110.5152/tjh.2011.05TJH-65872Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia majorSuleimman Ahmad Al-Sweedan0Said Jaradat1Khitam Amer2Wail Hayajneh3Hazem Haddad4Department Of Pediatrics, Jordan University Of Science And Technology, Irbid, JordanDepartment Of Genetics, Jordan University Of Science And Technology, Irbid, JordanDepartment Of Genetics, Jordan University Of Science And Technology, Irbid, JordanDepartment Of Pediatrics, Jordan University Of Science And Technology, Irbid, JordanDepartment Of Genetics, Jordan University Of Science And Technology, Irbid, JordanOBJECTIVE: The main objectives of this study are to investigate the prevalence of HCV among patients with β-thalassemia major and to determine the most prevalent genotype for this virus among them. METHODS: One hundred twenty-two β-thalassemia major patients who were previously diagnosed at the molecular level were included. All plasma samples were tested for the presence of antibodies by ELISA. Real-time polymerase chain reaction (PCR) was used in the quantitation the HCV RNA viral loads, and consequently, patients with high virus titer were genotyped by the linear array. RESULTS: Forty of the patients were anti-HCV positive. The prevalence of anti-HCV was significantly higher in patients who received blood transfusion before 1993 (83.7%) than in those who received it after 1993 (16.3%) (p=0.000). β-thalassemia major patients with HCV infection had significantly higher rates of elevated aspartate aminotransferase (54.4% vs 40.5%, p=0.045) and alanine aminotransferase (72.47% vs 37.47%, p=0.00) and of splenectomy (54.8% vs 45.2%, p=0.004) than β-thalassemia major patients without HCV. CONCLUSION: HCV genotype 4 is the commonest genotype in multi-transfused patients with β-thalassemia major in Jordan.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-65872virologytransfusionthalassemia |
| spellingShingle | Suleimman Ahmad Al-Sweedan Said Jaradat Khitam Amer Wail Hayajneh Hazem Haddad Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major Turkish Journal of Hematology virology transfusion thalassemia |
| title | Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major |
| title_full | Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major |
| title_fullStr | Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major |
| title_full_unstemmed | Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major |
| title_short | Seroprevalence and genotyping of hepatitis C virus in multiple transfused Jordanian patients with β-thalassemia major |
| title_sort | seroprevalence and genotyping of hepatitis c virus in multiple transfused jordanian patients with 946 thalassemia major |
| topic | virology transfusion thalassemia |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-65872 |
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