Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital
Abstract Aims: The increasing usage of antibiotics can cause resistance to the treatment of infections, which can caused by bacteria, e.g. Pseudomonas aeruginosa. The aim of this study was to trace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection and determin...
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| Format: | Article |
| Language: | English |
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Kashan University of Medical Sciences
2016-09-01
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| Series: | International Archives of Health Sciences |
| Subjects: | |
| Online Access: | http://iahs.kaums.ac.ir/browse.php?a_id=88&sid=1&slc_lang=en |
| _version_ | 1797668921582551040 |
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| author | Nourbakhsh F.* PhD, Momtaz H. PhD |
| author_facet | Nourbakhsh F.* PhD, Momtaz H. PhD |
| author_sort | Nourbakhsh F.* PhD, |
| collection | DOAJ |
| description | Abstract
Aims: The increasing usage of antibiotics can cause resistance to the treatment of infections, which can caused by bacteria, e.g. Pseudomonas aeruginosa. The aim of this study was to trace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection and determining the antibiotic resistance pattern of the bacteria.
Instrument & Methods: In this cross-sectional study, 100 Pseudomonas aeruginosa clinical isolates of infected wounds, bedsores, burns, urinary tract infections and respiratory tract infections were collected from patients of 3 Isfahan City hospitals, Iran (Al Zahra, Kashani, Shariati) in 2015. After identification tests and antibiogram, integrons class I, II and III were detected by M-PCR method. Data analysis were performed in SPSS 16 software using Chi-square and Fisher exact tests and the relationship between the presence of class III, II, I was calculated by M-PCR test.
Findings: All isolates had multiple antibiotic resistances. The highest antibiotic resistance was to Tetracycline (85%) and the lowest to Norfloxacin (12.5%). There were significant differences between class I and the two other classes of integrons (p=0.036). There was a statistically significant difference between the presence of blaTEM gene in Pseudomonas aeruginosa with other coding genes for antibiotic resistance (p=0.029).
Conclusion: Pseudomonas aeruginosa isolates are multi-drug resistant and almost all isolates from clinical infections have class I, II and III Integrons. |
| first_indexed | 2024-03-11T20:36:34Z |
| format | Article |
| id | doaj.art-ab5c6e7f5891460ead7537a31ca5d5a2 |
| institution | Directory Open Access Journal |
| issn | 2383-2568 |
| language | English |
| last_indexed | 2024-03-11T20:36:34Z |
| publishDate | 2016-09-01 |
| publisher | Kashan University of Medical Sciences |
| record_format | Article |
| series | International Archives of Health Sciences |
| spelling | doaj.art-ab5c6e7f5891460ead7537a31ca5d5a22023-10-02T06:17:54ZengKashan University of Medical SciencesInternational Archives of Health Sciences2383-25682016-09-0133125129Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial HospitalNourbakhsh F.* PhD,0Momtaz H. PhD1Department of Pharmacodynamy & Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Microbiology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, IranAbstract Aims: The increasing usage of antibiotics can cause resistance to the treatment of infections, which can caused by bacteria, e.g. Pseudomonas aeruginosa. The aim of this study was to trace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection and determining the antibiotic resistance pattern of the bacteria. Instrument & Methods: In this cross-sectional study, 100 Pseudomonas aeruginosa clinical isolates of infected wounds, bedsores, burns, urinary tract infections and respiratory tract infections were collected from patients of 3 Isfahan City hospitals, Iran (Al Zahra, Kashani, Shariati) in 2015. After identification tests and antibiogram, integrons class I, II and III were detected by M-PCR method. Data analysis were performed in SPSS 16 software using Chi-square and Fisher exact tests and the relationship between the presence of class III, II, I was calculated by M-PCR test. Findings: All isolates had multiple antibiotic resistances. The highest antibiotic resistance was to Tetracycline (85%) and the lowest to Norfloxacin (12.5%). There were significant differences between class I and the two other classes of integrons (p=0.036). There was a statistically significant difference between the presence of blaTEM gene in Pseudomonas aeruginosa with other coding genes for antibiotic resistance (p=0.029). Conclusion: Pseudomonas aeruginosa isolates are multi-drug resistant and almost all isolates from clinical infections have class I, II and III Integrons.http://iahs.kaums.ac.ir/browse.php?a_id=88&sid=1&slc_lang=enPseudomonas aeruginosaIntegronsMultiple Antibiotic ResistanceHospital Infections |
| spellingShingle | Nourbakhsh F.* PhD, Momtaz H. PhD Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital International Archives of Health Sciences Pseudomonas aeruginosa Integrons Multiple Antibiotic Resistance Hospital Infections |
| title | Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital |
| title_full | Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital |
| title_fullStr | Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital |
| title_full_unstemmed | Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital |
| title_short | Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital |
| title_sort | role of class i ii and iii integrons in multidrug resistance in pseudomonas aeruginosa isolated from nosocomial hospital |
| topic | Pseudomonas aeruginosa Integrons Multiple Antibiotic Resistance Hospital Infections |
| url | http://iahs.kaums.ac.ir/browse.php?a_id=88&sid=1&slc_lang=en |
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