Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells

Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells

Summary: Electron microscopy is the gold standard to characterize cellular ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. Here, we describe a semi-automated high-throughput strategy using single-axis serial section electron tomography to...

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Bibliographic Details
Main Authors: Sebastian Köhrer, Tobias Dittrich, Martin Schorb, Isabella Haberbosch, Yannick Schwab, Alwin Krämer
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
High Throughput Screening
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003404

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