Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide
<p>Abstract</p> <p>Background</p> <p>Huanglongbing (HLB) is one of the most destructive citrus diseases in the world. The disease is associated with the presence of a fastidious, phloem-limited α- proteobacterium, '<it>Candidatus </it>Liberibacter asiat...
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BMC
2012-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/39 |
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| author | Islam Md-Sajedul Glynn Jonathan M Bai Yang Duan Yong-Ping Coletta-Filho Helvecio D Kuruba Gopal Civerolo Edwin L Lin Hong |
| author_facet | Islam Md-Sajedul Glynn Jonathan M Bai Yang Duan Yong-Ping Coletta-Filho Helvecio D Kuruba Gopal Civerolo Edwin L Lin Hong |
| author_sort | Islam Md-Sajedul |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Huanglongbing (HLB) is one of the most destructive citrus diseases in the world. The disease is associated with the presence of a fastidious, phloem-limited α- proteobacterium, '<it>Candidatus </it>Liberibacter asiaticus', '<it>Ca</it>. Liberibacter africanus' or '<it>Ca</it>. Liberibacter americanus'. HLB-associated Liberibacters have spread to North America and South America in recent years. While the causal agents of HLB have been putatively identified, information regarding the worldwide population structure and epidemiological relationships for '<it>Ca</it>. L. asiaticus' is limited. The availability of the '<it>Ca</it>. L. asiaticus' genome sequence has facilitated development of molecular markers from this bacterium. The objectives of this study were to develop microsatellite markers and conduct genetic analyses of '<it>Ca</it>. L. asiaticus' from a worldwide collection. Two hundred eighty seven isolates from USA (Florida), Brazil, China, India, Cambodia, Vietnam, Taiwan, Thailand, and Japan were analyzed.</p> <p>Results</p> <p>A panel of seven polymorphic microsatellite markers was developed for '<it>Ca</it>. L. asiaticus'. Microsatellite analyses across the samples showed that the genetic diversity of '<it>Ca</it>. L. asiaticus' is higher in Asia than Americas. UPGMA and STRUCTURE analyses identified three major genetic groups worldwide. Isolates from India were genetically distinct. East-southeast Asian and Brazilian isolates were generally included in the same group; a few members of this group were found in Florida, but the majority of the isolates from Florida were clustered separately. eBURST analysis predicted three founder haplotypes, which may have given rise to three groups worldwide.</p> <p>Conclusions</p> <p>Our results identified three major genetic groups of '<it>Ca</it>. L. asiaticus' worldwide. Isolates from Brazil showed similar genetic makeup with east-southeast Asian dominant group, suggesting the possibility of a common origin. However, most of the isolates recovered from Florida were clustered in a separate group. While the sources of the dominant '<it>Ca</it>. L. asiaticus' in Florida were not clearly understood, the less-pervasive groups may have been introduced directly from Asia or via Brazil. Notably, the recent outbreak of HLB in Florida probably occurred through multiple introductions. Microsatellite markers developed in this study provide adequate discriminatory power for the identification and differentiation of closely-related isolates, as well as for genetic studies of '<it>Ca</it>. L. asiaticus'.</p> |
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| spelling | doaj.art-ab5e98e0397e4cbd9fe3aae1d0dfedaf2022-12-21T20:38:27ZengBMCBMC Microbiology1471-21802012-03-011213910.1186/1471-2180-12-39Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwideIslam Md-SajedulGlynn Jonathan MBai YangDuan Yong-PingColetta-Filho Helvecio DKuruba GopalCiverolo Edwin LLin Hong<p>Abstract</p> <p>Background</p> <p>Huanglongbing (HLB) is one of the most destructive citrus diseases in the world. The disease is associated with the presence of a fastidious, phloem-limited α- proteobacterium, '<it>Candidatus </it>Liberibacter asiaticus', '<it>Ca</it>. Liberibacter africanus' or '<it>Ca</it>. Liberibacter americanus'. HLB-associated Liberibacters have spread to North America and South America in recent years. While the causal agents of HLB have been putatively identified, information regarding the worldwide population structure and epidemiological relationships for '<it>Ca</it>. L. asiaticus' is limited. The availability of the '<it>Ca</it>. L. asiaticus' genome sequence has facilitated development of molecular markers from this bacterium. The objectives of this study were to develop microsatellite markers and conduct genetic analyses of '<it>Ca</it>. L. asiaticus' from a worldwide collection. Two hundred eighty seven isolates from USA (Florida), Brazil, China, India, Cambodia, Vietnam, Taiwan, Thailand, and Japan were analyzed.</p> <p>Results</p> <p>A panel of seven polymorphic microsatellite markers was developed for '<it>Ca</it>. L. asiaticus'. Microsatellite analyses across the samples showed that the genetic diversity of '<it>Ca</it>. L. asiaticus' is higher in Asia than Americas. UPGMA and STRUCTURE analyses identified three major genetic groups worldwide. Isolates from India were genetically distinct. East-southeast Asian and Brazilian isolates were generally included in the same group; a few members of this group were found in Florida, but the majority of the isolates from Florida were clustered separately. eBURST analysis predicted three founder haplotypes, which may have given rise to three groups worldwide.</p> <p>Conclusions</p> <p>Our results identified three major genetic groups of '<it>Ca</it>. L. asiaticus' worldwide. Isolates from Brazil showed similar genetic makeup with east-southeast Asian dominant group, suggesting the possibility of a common origin. However, most of the isolates recovered from Florida were clustered in a separate group. While the sources of the dominant '<it>Ca</it>. L. asiaticus' in Florida were not clearly understood, the less-pervasive groups may have been introduced directly from Asia or via Brazil. Notably, the recent outbreak of HLB in Florida probably occurred through multiple introductions. Microsatellite markers developed in this study provide adequate discriminatory power for the identification and differentiation of closely-related isolates, as well as for genetic studies of '<it>Ca</it>. L. asiaticus'.</p>http://www.biomedcentral.com/1471-2180/12/39 |
| spellingShingle | Islam Md-Sajedul Glynn Jonathan M Bai Yang Duan Yong-Ping Coletta-Filho Helvecio D Kuruba Gopal Civerolo Edwin L Lin Hong Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide BMC Microbiology |
| title | Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide |
| title_full | Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide |
| title_fullStr | Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide |
| title_full_unstemmed | Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide |
| title_short | Multilocus microsatellite analysis of '<it>Candidatus </it>Liberibacter asiaticus' associated with citrus Huanglongbing worldwide |
| title_sort | multilocus microsatellite analysis of it candidatus it liberibacter asiaticus associated with citrus huanglongbing worldwide |
| url | http://www.biomedcentral.com/1471-2180/12/39 |
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