The Effect of Different Concentrations of Growth Regulators and the Type of Explants on Embryogenesis and Rooting of German Chamomile (Matricaria chamomilla L)
Chamomile (Matricaria chamomilla L.) is one of the nine important medicinal plants found in the world. In this study, the effect of different concentrations of plant growth regulators and explants has been investigated on embryogenesis and rooting of German chamomile (Matricaria spp.). MS culture...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-09-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/the-effect-of-different-concentrations-of-growth-regulators-and-the-type-of-explants-on-embryogenesis-and-rooting-of-german-chamomile-matricaria-chamomilla-l/ |
| Summary: | Chamomile (Matricaria chamomilla L.) is one of the nine important medicinal
plants found in the world. In this study, the effect of different concentrations of plant growth
regulators and explants has been investigated on embryogenesis and rooting of German
chamomile (Matricaria spp.). MS culture medium (Murashige Skoog., 1986) was the basal
medium in this experiment. Factorial experiments were conducted in a Randomized Complete
Block Design with three replications. In testing the effect of 1-Naphthaleneacetic acid (NAA) and
Kinetin on the embryogenesis, factors included: (NAA) at four levels (0, 0.5, 1, 2 mg/l), Kinetin
at four levels (0, 0.5, 1, 2 mg/l) and explants in three levels: Stem, Axillary buds and leaves.
In investigating the effect of NAA and Kinetin on rooting, factors included NAA at four levels:
(0, 0.5, 1, 2 mg/l), Kinetin at four levels: (0, 0.5, 1, 2 mg/l). Results showed that leaf explants in
culture medium containing a treatment Al: (NAA: 1 +Kin: 1mg/l) and stem explants in culture
medium containing a treatment A2: (NAA: 1 +Kin: 0.5 mg/l) and the Axillary bud explants in
culture medium containing hormonal composition of A3: (NAA: 0.5 +Kin: 1mg/l) produced the
highest embryogenic calli. In terms of rooting, the culture medium containing the treatment
A4: (NAA: 1 +Kin: 0 mg/l) created the highest rooting. |
|---|---|
| ISSN: | 0973-7510 2581-690X |