The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development
Seed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is sti...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/20/11403 |
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| author | Ekaterini Koura Adamantia Pistikoudi Margaritis Tsifintaris George Tsiolas Evangelia Mouchtaropoulou Christos Noutsos Triantafyllos Karantakis Athanasios Kouras Athanasios Karanikolas Anagnostis Argiriou Irini Nianiou-Obeidat Photini V. Mylona Alexios N. Polidoros |
| author_facet | Ekaterini Koura Adamantia Pistikoudi Margaritis Tsifintaris George Tsiolas Evangelia Mouchtaropoulou Christos Noutsos Triantafyllos Karantakis Athanasios Kouras Athanasios Karanikolas Anagnostis Argiriou Irini Nianiou-Obeidat Photini V. Mylona Alexios N. Polidoros |
| author_sort | Ekaterini Koura |
| collection | DOAJ |
| description | Seed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is still under research. Through RNA sequencing, this study explored the effect of three P treatments (P0, 6 mg kg<sup>−1</sup>; P1, 15 mg kg<sup>−1</sup>; and P2, 21 mg kg<sup>−1</sup>) and three seed maturity stages (S1, immature 2 mm seed in a flat pod; S2, fully developed seed within the pod; and S3, mature seed at the beginning of the pod’s discoloration) on lentil gene expression. The key findings highlighted a significant influence of the seed maturity stage on phenylpropanoid genes, with S1 displaying the highest expression levels, and on phosphorus-related Gene Ontology (GO) terms that presented the highest number of downregulated genes in the S3 to S1 comparison. P exhibited a targeted effect on the flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) genes and specific gene clusters, as shown by the differential gene expression analysis. This study investigates the molecular mechanisms related to phosphorus fertilization and seed maturity stages that influence tannin accumulation, offering valuable information for the enhancement of lentil product quality through breeding programs. |
| first_indexed | 2024-03-10T21:28:37Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T21:28:37Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-ab65fa562c45459b915142bc56a1d2f02023-11-19T15:31:45ZengMDPI AGApplied Sciences2076-34172023-10-0113201140310.3390/app132011403The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed DevelopmentEkaterini Koura0Adamantia Pistikoudi1Margaritis Tsifintaris2George Tsiolas3Evangelia Mouchtaropoulou4Christos Noutsos5Triantafyllos Karantakis6Athanasios Kouras7Athanasios Karanikolas8Anagnostis Argiriou9Irini Nianiou-Obeidat10Photini V. Mylona11Alexios N. Polidoros12School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Molecular Biology and Genetics, School of Life Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceInstitute of Applied Biosciences, Center for Research and Technology Hellas, 57001 Thermi, GreeceInstitute of Applied Biosciences, Center for Research and Technology Hellas, 57001 Thermi, GreeceBiology Department, SUNY College at Old Westbury, New York, NY 11731, USASchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Applied Biosciences, Center for Research and Technology Hellas, 57001 Thermi, GreeceSchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization-DEMETER, 57001 Thermi, GreeceSchool of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSeed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is still under research. Through RNA sequencing, this study explored the effect of three P treatments (P0, 6 mg kg<sup>−1</sup>; P1, 15 mg kg<sup>−1</sup>; and P2, 21 mg kg<sup>−1</sup>) and three seed maturity stages (S1, immature 2 mm seed in a flat pod; S2, fully developed seed within the pod; and S3, mature seed at the beginning of the pod’s discoloration) on lentil gene expression. The key findings highlighted a significant influence of the seed maturity stage on phenylpropanoid genes, with S1 displaying the highest expression levels, and on phosphorus-related Gene Ontology (GO) terms that presented the highest number of downregulated genes in the S3 to S1 comparison. P exhibited a targeted effect on the flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) genes and specific gene clusters, as shown by the differential gene expression analysis. This study investigates the molecular mechanisms related to phosphorus fertilization and seed maturity stages that influence tannin accumulation, offering valuable information for the enhancement of lentil product quality through breeding programs.https://www.mdpi.com/2076-3417/13/20/11403RNA sequencingdifferential gene expressionphenylpropanoid pathwaytanninsproanthocyanidinsRT-qPCR |
| spellingShingle | Ekaterini Koura Adamantia Pistikoudi Margaritis Tsifintaris George Tsiolas Evangelia Mouchtaropoulou Christos Noutsos Triantafyllos Karantakis Athanasios Kouras Athanasios Karanikolas Anagnostis Argiriou Irini Nianiou-Obeidat Photini V. Mylona Alexios N. Polidoros The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development Applied Sciences RNA sequencing differential gene expression phenylpropanoid pathway tannins proanthocyanidins RT-qPCR |
| title | The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development |
| title_full | The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development |
| title_fullStr | The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development |
| title_full_unstemmed | The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development |
| title_short | The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development |
| title_sort | effect of phosphorus fertilization on transcriptome expression profile during lentil pod and seed development |
| topic | RNA sequencing differential gene expression phenylpropanoid pathway tannins proanthocyanidins RT-qPCR |
| url | https://www.mdpi.com/2076-3417/13/20/11403 |
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