Production and Stabilization of Specific Upregulated Long Noncoding RNA HOXD-AS2 in Glioblastomas Are Mediated by TFE3 and miR-661, Respectively
Differential expression of long noncoding RNAs (lncRNA) plays a key role in the development of gliomas. Because gliomas are the most common primary central nervous system tumor and glioblastomas have poor prognosis, it is urgent to develop new diagnostic methods. We have previously reported that lnc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/5/2828 |
| Summary: | Differential expression of long noncoding RNAs (lncRNA) plays a key role in the development of gliomas. Because gliomas are the most common primary central nervous system tumor and glioblastomas have poor prognosis, it is urgent to develop new diagnostic methods. We have previously reported that lncRNA <i>HOXD-AS2</i>, which is specifically up-regulated in gliomas, can activate cell cycle and promote the development of gliomas. It is expected to be a new marker for molecular diagnosis of gliomas, but little is known about <i>HOXD-AS2</i>. Here, we demonstrate that <i>TFE3</i> and <i>miR-661</i> maintain the high expression level of <i>HOXD-AS2</i> by regulating its production and degradation. We found that <i>TFE3</i> acted as a transcription factor binding to the <i>HOXD-AS2</i> promoter region and raised H3K27ac to activate <i>HOXD-AS2</i>. As the cytoplasmic-located lncRNA, <i>HOXD-AS2</i> could be degraded by <i>miR-661</i>. This process was inhibited in gliomas due to the low expression of <i>miR-661</i>. Our study explains why <i>HOXD-AS2</i> was specifically up-regulated in gliomas, helps to understand the molecular characteristics of gliomas, and provids insights for the search for specific markers in gliomas. |
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| ISSN: | 1661-6596 1422-0067 |