Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Departmen...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2012-10-01
|
| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/controlled-gentamicin-release-from-multi-layered-electrospun-nanofibro-a11205 |
| _version_ | 1818421410204221440 |
|---|---|
| author | Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J |
| author_facet | Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J |
| author_sort | Sirc J |
| collection | DOAJ |
| description | Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic; 3Elmarco Ltd, Liberec, Czech Republic; 4Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic; 5Department of Transplant Immunology, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech RepublicAbstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.Keywords: nanofibers, electrospinning, multilayered structure, morphology, gentamicin, drug release |
| first_indexed | 2024-12-14T13:09:55Z |
| format | Article |
| id | doaj.art-ab68077eaf6746e780f28db300bcd18e |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T13:09:55Z |
| publishDate | 2012-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-ab68077eaf6746e780f28db300bcd18e2022-12-21T23:00:14ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132012-10-012012default53155325Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknessesSirc JKubinova SHobzova RStranska DKozlik PBosakova ZMarekova DHolan VSykova EMichalek JJakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic; 3Elmarco Ltd, Liberec, Czech Republic; 4Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic; 5Department of Transplant Immunology, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech RepublicAbstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.Keywords: nanofibers, electrospinning, multilayered structure, morphology, gentamicin, drug releasehttp://www.dovepress.com/controlled-gentamicin-release-from-multi-layered-electrospun-nanofibro-a11205 |
| spellingShingle | Sirc J Kubinova S Hobzova R Stranska D Kozlik P Bosakova Z Marekova D Holan V Sykova E Michalek J Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses International Journal of Nanomedicine |
| title | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses |
| title_full | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses |
| title_fullStr | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses |
| title_full_unstemmed | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses |
| title_short | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses |
| title_sort | controlled gentamicin release from multi layered electrospun nanofibrous structures of various thicknesses |
| url | http://www.dovepress.com/controlled-gentamicin-release-from-multi-layered-electrospun-nanofibro-a11205 |
| work_keys_str_mv | AT sircj controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT kubinovas controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT hobzovar controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT stranskad controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT kozlikp controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT bosakovaz controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT marekovad controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT holanv controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT sykovae controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses AT michalekj controlledgentamicinreleasefrommultilayeredelectrospunnanofibrousstructuresofvariousthicknesses |