Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids
Cytosolic lipid droplets (LDs) store excess fatty acids (FAs) in the form of neutral lipids and prevent starvation-induced cancer cell death. Here we studied the ability of mono- and polyunsaturated FAs to affect LD formation and survival in Hela cervical cancer cells. We found that the LD content i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Slovenian Chemical Society
2017-09-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/2908 |
| _version_ | 1811198747417247744 |
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| author | Ema Guštin Eva Jarc Ana Kump Toni Petan |
| author_facet | Ema Guštin Eva Jarc Ana Kump Toni Petan |
| author_sort | Ema Guštin |
| collection | DOAJ |
| description | Cytosolic lipid droplets (LDs) store excess fatty acids (FAs) in the form of neutral lipids and prevent starvation-induced cancer cell death. Here we studied the ability of mono- and polyunsaturated FAs to affect LD formation and survival in Hela cervical cancer cells. We found that the LD content in Hela cells increases with cell density, but it decreases in MDA-MB-231 breast cancer cells. Exogenously-added unsaturated FAs, including oleic (OA), linoleic (LA), arachidonic (AA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), displayed a similar ability to alter LD formation in Hela cells. There was a dual, concentration-dependent effect: low micromolar concentrations suppressed, while higher concentrations of FAs induced LD formation. In serum starved Hela cells, OA stimulated LD formation, but, contrary to expectations, it promoted cell death. Our results reveal a link between cell population density and LD formation in Hela cells and show that unsaturated FAs may both suppress and stimulate LD formation. This dynamic regulation of LD content must be accounted for when studying the effects of lipids and lipid metabolism-targeting drugs on LD metabolism in HeLa cells. |
| first_indexed | 2024-04-12T01:36:29Z |
| format | Article |
| id | doaj.art-ab6a63ea1732446393433c8c98a98ff5 |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-04-12T01:36:29Z |
| publishDate | 2017-09-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-ab6a63ea1732446393433c8c98a98ff52022-12-22T03:53:18ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552017-09-0164354955410.17344/acsi.2016.2908457Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acidsEma GuštinEva JarcAna KumpToni PetanCytosolic lipid droplets (LDs) store excess fatty acids (FAs) in the form of neutral lipids and prevent starvation-induced cancer cell death. Here we studied the ability of mono- and polyunsaturated FAs to affect LD formation and survival in Hela cervical cancer cells. We found that the LD content in Hela cells increases with cell density, but it decreases in MDA-MB-231 breast cancer cells. Exogenously-added unsaturated FAs, including oleic (OA), linoleic (LA), arachidonic (AA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), displayed a similar ability to alter LD formation in Hela cells. There was a dual, concentration-dependent effect: low micromolar concentrations suppressed, while higher concentrations of FAs induced LD formation. In serum starved Hela cells, OA stimulated LD formation, but, contrary to expectations, it promoted cell death. Our results reveal a link between cell population density and LD formation in Hela cells and show that unsaturated FAs may both suppress and stimulate LD formation. This dynamic regulation of LD content must be accounted for when studying the effects of lipids and lipid metabolism-targeting drugs on LD metabolism in HeLa cells.https://journals.matheo.si/index.php/ACSi/article/view/2908lipid dropletslipid metabolismcell densityHeLa cervical cancer cellsunsaturated fatty acidsapoptosis |
| spellingShingle | Ema Guštin Eva Jarc Ana Kump Toni Petan Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids Acta Chimica Slovenica lipid droplets lipid metabolism cell density HeLa cervical cancer cells unsaturated fatty acids apoptosis |
| title | Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| title_full | Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| title_fullStr | Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| title_full_unstemmed | Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| title_short | Lipid droplet formation in HeLa cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| title_sort | lipid droplet formation in hela cervical cancer cells depends on cell density and the concentration of exogenous unsaturated fatty acids |
| topic | lipid droplets lipid metabolism cell density HeLa cervical cancer cells unsaturated fatty acids apoptosis |
| url | https://journals.matheo.si/index.php/ACSi/article/view/2908 |
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