Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry
A laminar flow bioelectrochemical systems (BES) was designed and benchmarked using microbial anodes dominated with Geobacter spp. The reactor architecture was based on modeled flow fields, the resulting structure was 3D printed and used for BES manufacturing. Stratification of the substrate availabi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Environmental Science and Ecotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666498420300545 |
| _version_ | 1818677516077891584 |
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| author | Yuting Guo Luis F.M. Rosa Susann Müller Falk Harnisch |
| author_facet | Yuting Guo Luis F.M. Rosa Susann Müller Falk Harnisch |
| author_sort | Yuting Guo |
| collection | DOAJ |
| description | A laminar flow bioelectrochemical systems (BES) was designed and benchmarked using microbial anodes dominated with Geobacter spp. The reactor architecture was based on modeled flow fields, the resulting structure was 3D printed and used for BES manufacturing. Stratification of the substrate availability within the reactor channels led to heterogeneous biomass distribution, with the maximum biomass found mainly in the initial/middle channels. The anode performance was assessed for different hydraulic retention times while coulombic efficiencies of up to 100% (including also hydrogen recycling from the cathode) and current densities of up to 75 μA cm−2 at an anode surface to volume ratio of 1770 cm2 L−1 after 35 days were achieved. This low current density can be clearly attributed to the heterogeneous distributions of biomass and the stratification of the microbial community structure. Further, it was shown that time and space resolved analysis of the reactor microbiomes per channel is feasible using flow cytometry. |
| first_indexed | 2024-12-17T09:00:36Z |
| format | Article |
| id | doaj.art-ab6a80fcf196437d9fe2e975b71d0e05 |
| institution | Directory Open Access Journal |
| issn | 2666-4984 |
| language | English |
| last_indexed | 2024-12-17T09:00:36Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Science and Ecotechnology |
| spelling | doaj.art-ab6a80fcf196437d9fe2e975b71d0e052022-12-21T21:55:45ZengElsevierEnvironmental Science and Ecotechnology2666-49842020-10-014100062Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometryYuting Guo0Luis F.M. Rosa1Susann Müller2Falk Harnisch3Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research – UFZ, Permoserstrasse 15, 04318, Leipzig, GermanyDepartment of Environmental Microbiology, Helmholtz-Centre for Environmental Research – UFZ, Permoserstrasse 15, 04318, Leipzig, GermanyDepartment of Environmental Microbiology, Helmholtz-Centre for Environmental Research – UFZ, Permoserstrasse 15, 04318, Leipzig, GermanyCorresponding author.; Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research – UFZ, Permoserstrasse 15, 04318, Leipzig, GermanyA laminar flow bioelectrochemical systems (BES) was designed and benchmarked using microbial anodes dominated with Geobacter spp. The reactor architecture was based on modeled flow fields, the resulting structure was 3D printed and used for BES manufacturing. Stratification of the substrate availability within the reactor channels led to heterogeneous biomass distribution, with the maximum biomass found mainly in the initial/middle channels. The anode performance was assessed for different hydraulic retention times while coulombic efficiencies of up to 100% (including also hydrogen recycling from the cathode) and current densities of up to 75 μA cm−2 at an anode surface to volume ratio of 1770 cm2 L−1 after 35 days were achieved. This low current density can be clearly attributed to the heterogeneous distributions of biomass and the stratification of the microbial community structure. Further, it was shown that time and space resolved analysis of the reactor microbiomes per channel is feasible using flow cytometry.http://www.sciencedirect.com/science/article/pii/S2666498420300545Microbial electrochemical technologyFlow cytometryLaminar flowMicrobial communityElectroactive microorganisms |
| spellingShingle | Yuting Guo Luis F.M. Rosa Susann Müller Falk Harnisch Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry Environmental Science and Ecotechnology Microbial electrochemical technology Flow cytometry Laminar flow Microbial community Electroactive microorganisms |
| title | Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| title_full | Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| title_fullStr | Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| title_full_unstemmed | Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| title_short | Monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| title_sort | monitoring stratification of anode biofilms in bioelectrochemical laminar flow reactors using flow cytometry |
| topic | Microbial electrochemical technology Flow cytometry Laminar flow Microbial community Electroactive microorganisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666498420300545 |
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