A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets
In an attempt to find a potential application of cell culture harvesting, a novel method for the preparation of an upper critical solution temperature (UCST) thermosensitive hydrogel was studied. An electron accelerator was used as the electron beam (EB) radiation source, and acrylamide (AAm) was fi...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/6/345 |
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| author | Yi Ling Liuyuchen Chen Mingjun Huang Cheng Zhou Liming Yang Hejingying Niu Li Su Yuejiao Yang Rogério P. Pirraco Rui L. Reis Jie Chen |
| author_facet | Yi Ling Liuyuchen Chen Mingjun Huang Cheng Zhou Liming Yang Hejingying Niu Li Su Yuejiao Yang Rogério P. Pirraco Rui L. Reis Jie Chen |
| author_sort | Yi Ling |
| collection | DOAJ |
| description | In an attempt to find a potential application of cell culture harvesting, a novel method for the preparation of an upper critical solution temperature (UCST) thermosensitive hydrogel was studied. An electron accelerator was used as the electron beam (EB) radiation source, and acrylamide (AAm) was first grafted onto the pre-irradiated polypropylene (PP) sheet. Then, the grafting layer of poly (acrylamide-co-acrylonitrile) (P (AAm-co-AN)) was obtained by the partial dehydration of the acylamino group into the cyano group in the solution mixture of sulfoxide chloride (SOCl<sub>2</sub>) and dimethyl formamide (DMF). The effects of the absorbed dose, AAm concentration, reaction time, and temperature on the degree of grafting were studied, respectively. The effect of the SOCl<sub>2</sub> concentration on the conversion degree of the cyano group from the acylamino group was studied, followed by the temperature of the UCST. The UCST properties of the grafted samples with P (AAm-co-AN) were studied by quartz crystal microbalance (QCM) and atomic force microscope (AFM), respectively. The cytotoxicities of the hydrogels against cells were verified by CCK-8 studies. |
| first_indexed | 2024-03-09T23:45:01Z |
| format | Article |
| id | doaj.art-ab712e2c8f1b4eb8b90c66be9f23e4ff |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T23:45:01Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-ab712e2c8f1b4eb8b90c66be9f23e4ff2023-11-23T16:45:19ZengMDPI AGGels2310-28612022-05-018634510.3390/gels8060345A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene SheetsYi Ling0Liuyuchen Chen1Mingjun Huang2Cheng Zhou3Liming Yang4Hejingying Niu5Li Su6Yuejiao Yang7Rogério P. Pirraco8Rui L. Reis9Jie Chen10Department of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaSchool of Life Sciences, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, China3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark—Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark—Zona Industrial da Gandra, Barco, 4805-017 Guimarães, PortugalDepartment of Chemical Engineering and Technology, School of Environmental and Chemical Engineering, Shanghai University, Shangda Road 99, Shanghai 200444, ChinaIn an attempt to find a potential application of cell culture harvesting, a novel method for the preparation of an upper critical solution temperature (UCST) thermosensitive hydrogel was studied. An electron accelerator was used as the electron beam (EB) radiation source, and acrylamide (AAm) was first grafted onto the pre-irradiated polypropylene (PP) sheet. Then, the grafting layer of poly (acrylamide-co-acrylonitrile) (P (AAm-co-AN)) was obtained by the partial dehydration of the acylamino group into the cyano group in the solution mixture of sulfoxide chloride (SOCl<sub>2</sub>) and dimethyl formamide (DMF). The effects of the absorbed dose, AAm concentration, reaction time, and temperature on the degree of grafting were studied, respectively. The effect of the SOCl<sub>2</sub> concentration on the conversion degree of the cyano group from the acylamino group was studied, followed by the temperature of the UCST. The UCST properties of the grafted samples with P (AAm-co-AN) were studied by quartz crystal microbalance (QCM) and atomic force microscope (AFM), respectively. The cytotoxicities of the hydrogels against cells were verified by CCK-8 studies.https://www.mdpi.com/2310-2861/8/6/345pre-irradiation graftingP (AAm-co-AN)UCSTthermosensitivecytotoxicity |
| spellingShingle | Yi Ling Liuyuchen Chen Mingjun Huang Cheng Zhou Liming Yang Hejingying Niu Li Su Yuejiao Yang Rogério P. Pirraco Rui L. Reis Jie Chen A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets Gels pre-irradiation grafting P (AAm-co-AN) UCST thermosensitive cytotoxicity |
| title | A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets |
| title_full | A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets |
| title_fullStr | A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets |
| title_full_unstemmed | A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets |
| title_short | A Novel Method for the Preparation of Poly (Acrylamide-co-Acrylonitrile) Upper Critical Solution Temperature Thermosensitive Hydrogel by the Partial Dehydration of Acrylamide Grafted Polypropylene Sheets |
| title_sort | novel method for the preparation of poly acrylamide co acrylonitrile upper critical solution temperature thermosensitive hydrogel by the partial dehydration of acrylamide grafted polypropylene sheets |
| topic | pre-irradiation grafting P (AAm-co-AN) UCST thermosensitive cytotoxicity |
| url | https://www.mdpi.com/2310-2861/8/6/345 |
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