<i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli
Lichen phycobiomes have recently emerged as a source of biodiversity and new species of microalgae. Although in the genus <i>Diplosphaera</i> free-living microalgae are common, numerous strains belonging to this genus have frequently been recognized or isolated from lichen thalli. In thi...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/1424-2818/15/2/168 |
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| author | Salvador Chiva César Daniel Bordenave Ayelén Gázquez Eva Barreno |
| author_facet | Salvador Chiva César Daniel Bordenave Ayelén Gázquez Eva Barreno |
| author_sort | Salvador Chiva |
| collection | DOAJ |
| description | Lichen phycobiomes have recently emerged as a source of biodiversity and new species of microalgae. Although in the genus <i>Diplosphaera</i> free-living microalgae are common, numerous strains belonging to this genus have frequently been recognized or isolated from lichen thalli. In this study, a comprehensive analysis of the strain <i>Diplosphaera</i> sp. ASUV135, isolated from a lichen thallus, has been carried out using an integrative taxonomic approach. The SSU and nuclear-encoding ITS rDNA, as well as the chloroplast <i>rbc</i>L gene, were sequenced and analyzed to ascertain its taxonomic position and phylogenetic relationships within the genus <i>Diplosphaera</i>. This strain was also analyzed by light, confocal and transmission microscopy for morphological and ultrastructural characterization. The phenotypic plasticity in this strain was also confirmed by changes in its morphology under different growth conditions, as well as those of modulated Chlorophyll <i>a</i> fluorescence emissions, to understand its photosynthetic functioning. Our results pointed out that this strain represents a new taxon within the genus <i>Diplosphaera</i> (<i>Prasiola</i> group), described here as <i>Diplosphaera elongate</i> sp. nova. This study also provides tools for future research on organisms with high phenotypic plasticity by using molecular, morphological, ultrastructural and physiological approaches. |
| first_indexed | 2024-03-11T08:57:13Z |
| format | Article |
| id | doaj.art-ab72484612494ba58556a5e0bf3fd007 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T08:57:13Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-ab72484612494ba58556a5e0bf3fd0072023-11-16T20:04:00ZengMDPI AGDiversity1424-28182023-01-0115216810.3390/d15020168<i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen ThalliSalvador Chiva0César Daniel Bordenave1Ayelén Gázquez2Eva Barreno3Department of Life Sciences, University of Trieste, Via L. Giorgieri 10, 34127 Trieste, ItalyFac. CC. Biológicas, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, ICBIBE, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainFac. CC. Biológicas, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, ICBIBE, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainFac. CC. Biológicas, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, ICBIBE, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainLichen phycobiomes have recently emerged as a source of biodiversity and new species of microalgae. Although in the genus <i>Diplosphaera</i> free-living microalgae are common, numerous strains belonging to this genus have frequently been recognized or isolated from lichen thalli. In this study, a comprehensive analysis of the strain <i>Diplosphaera</i> sp. ASUV135, isolated from a lichen thallus, has been carried out using an integrative taxonomic approach. The SSU and nuclear-encoding ITS rDNA, as well as the chloroplast <i>rbc</i>L gene, were sequenced and analyzed to ascertain its taxonomic position and phylogenetic relationships within the genus <i>Diplosphaera</i>. This strain was also analyzed by light, confocal and transmission microscopy for morphological and ultrastructural characterization. The phenotypic plasticity in this strain was also confirmed by changes in its morphology under different growth conditions, as well as those of modulated Chlorophyll <i>a</i> fluorescence emissions, to understand its photosynthetic functioning. Our results pointed out that this strain represents a new taxon within the genus <i>Diplosphaera</i> (<i>Prasiola</i> group), described here as <i>Diplosphaera elongate</i> sp. nova. This study also provides tools for future research on organisms with high phenotypic plasticity by using molecular, morphological, ultrastructural and physiological approaches.https://www.mdpi.com/1424-2818/15/2/168descriptionmodulated fluorometryphycobiomephylogeny<i>Stichococcus</i>-like organismultrastructures |
| spellingShingle | Salvador Chiva César Daniel Bordenave Ayelén Gázquez Eva Barreno <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli Diversity description modulated fluorometry phycobiome phylogeny <i>Stichococcus</i>-like organism ultrastructures |
| title | <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli |
| title_full | <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli |
| title_fullStr | <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli |
| title_full_unstemmed | <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli |
| title_short | <i>Diplosphaera elongata</i> sp. nova: Morphology and Phenotypic Plasticity of This New Microalga Isolated from Lichen Thalli |
| title_sort | i diplosphaera elongata i sp nova morphology and phenotypic plasticity of this new microalga isolated from lichen thalli |
| topic | description modulated fluorometry phycobiome phylogeny <i>Stichococcus</i>-like organism ultrastructures |
| url | https://www.mdpi.com/1424-2818/15/2/168 |
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