The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells.
The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-08-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008761 |
| _version_ | 1818717006958952448 |
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| author | Nicolás M Reinoso-Vizcaíno Melina B Cian Paulo R Cortes Nadia B Olivero Mirelys Hernandez-Morfa Germán E Piñas Chandan Badapanda Ankita Rathore Daniel R Perez José Echenique |
| author_facet | Nicolás M Reinoso-Vizcaíno Melina B Cian Paulo R Cortes Nadia B Olivero Mirelys Hernandez-Morfa Germán E Piñas Chandan Badapanda Ankita Rathore Daniel R Perez José Echenique |
| author_sort | Nicolás M Reinoso-Vizcaíno |
| collection | DOAJ |
| description | The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system SirRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the SirRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes. |
| first_indexed | 2024-12-17T19:28:18Z |
| format | Article |
| id | doaj.art-ab734bcf423d4b4196a38de2df195b73 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-17T19:28:18Z |
| publishDate | 2020-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-ab734bcf423d4b4196a38de2df195b732022-12-21T21:35:20ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-08-01168e100876110.1371/journal.ppat.1008761The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells.Nicolás M Reinoso-VizcaínoMelina B CianPaulo R CortesNadia B OliveroMirelys Hernandez-MorfaGermán E PiñasChandan BadapandaAnkita RathoreDaniel R PerezJosé EcheniqueThe virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system SirRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the SirRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.https://doi.org/10.1371/journal.ppat.1008761 |
| spellingShingle | Nicolás M Reinoso-Vizcaíno Melina B Cian Paulo R Cortes Nadia B Olivero Mirelys Hernandez-Morfa Germán E Piñas Chandan Badapanda Ankita Rathore Daniel R Perez José Echenique The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. PLoS Pathogens |
| title | The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. |
| title_full | The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. |
| title_fullStr | The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. |
| title_full_unstemmed | The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. |
| title_short | The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells. |
| title_sort | pneumococcal two component system sirrh is linked to enhanced intracellular survival of streptococcus pneumoniae in influenza infected pulmonary cells |
| url | https://doi.org/10.1371/journal.ppat.1008761 |
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