Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle
Abstract Bovine leukemia virus (BLV) causes enzootic bovine leucosis and is widely spread worldwide, except several European countries, Australia and New Zealand. Although BLV is highly prevalent in China, information about the genetic diversity and evolutionary dynamics of BLV among Chinese dairy h...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-019-1207-8 |
| _version_ | 1818504795588132864 |
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| author | Yi Yang Lina Chen Maoli Dong Wenjiang Huang Xiaoli Hao Yalan Peng Zaicheng Gong Aijian Qin Shaobin Shang Zhangping Yang |
| author_facet | Yi Yang Lina Chen Maoli Dong Wenjiang Huang Xiaoli Hao Yalan Peng Zaicheng Gong Aijian Qin Shaobin Shang Zhangping Yang |
| author_sort | Yi Yang |
| collection | DOAJ |
| description | Abstract Bovine leukemia virus (BLV) causes enzootic bovine leucosis and is widely spread worldwide, except several European countries, Australia and New Zealand. Although BLV is highly prevalent in China, information about the genetic diversity and evolutionary dynamics of BLV among Chinese dairy herds is still lacking. To determine the genetic variability of BLV, 219 cows from four cities of Ningxia province of China were screened for BLV infection by fluorescence resonance energy transfer (FRET)-PCR and sequencing, 16 selected positive samples were subjected to molecular characterization. Phylogenetic analysis using the neighbor-joining (NJ) method on complete sequences of envelope (env) gene of BLV obtained from China and those available in GenBank (representing BLV genotypes 1–10) revealed that those Chinese strains belonged to genotypes 4 and 6. Totally, 23 mutations were identified and 16 of them were determined to be unique mutations among Chinese strains. Alignment of the deduced amino acid sequences demonstrated six mutations in glycoprotein 51 (gp51) and three mutations in glycoprotein 30 (gp30) located in the identified neutralizing domain (ND), CD8+ T cell epitope, E-epitope, B-epitope, gp51N12 and cytoplasmic domain of transmembrane protein. This study reported for the first time the BLV genotype 4 in China, and further studies are warranted to compare its immunogenicity and pathogenicity with other BLV genotypes. |
| first_indexed | 2024-12-10T21:42:05Z |
| format | Article |
| id | doaj.art-ab739aba4032475daa58afd1d2d993ea |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-10T21:42:05Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-ab739aba4032475daa58afd1d2d993ea2022-12-22T01:32:29ZengBMCVirology Journal1743-422X2019-08-011611710.1186/s12985-019-1207-8Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattleYi Yang0Lina Chen1Maoli Dong2Wenjiang Huang3Xiaoli Hao4Yalan Peng5Zaicheng Gong6Aijian Qin7Shaobin Shang8Zhangping Yang9Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; College of Veterinary Medicine, Yangzhou UniversityInternational Corporation Laboratory of Agriculture and Agricultural Products Safety, Yangzhou UniversityAbstract Bovine leukemia virus (BLV) causes enzootic bovine leucosis and is widely spread worldwide, except several European countries, Australia and New Zealand. Although BLV is highly prevalent in China, information about the genetic diversity and evolutionary dynamics of BLV among Chinese dairy herds is still lacking. To determine the genetic variability of BLV, 219 cows from four cities of Ningxia province of China were screened for BLV infection by fluorescence resonance energy transfer (FRET)-PCR and sequencing, 16 selected positive samples were subjected to molecular characterization. Phylogenetic analysis using the neighbor-joining (NJ) method on complete sequences of envelope (env) gene of BLV obtained from China and those available in GenBank (representing BLV genotypes 1–10) revealed that those Chinese strains belonged to genotypes 4 and 6. Totally, 23 mutations were identified and 16 of them were determined to be unique mutations among Chinese strains. Alignment of the deduced amino acid sequences demonstrated six mutations in glycoprotein 51 (gp51) and three mutations in glycoprotein 30 (gp30) located in the identified neutralizing domain (ND), CD8+ T cell epitope, E-epitope, B-epitope, gp51N12 and cytoplasmic domain of transmembrane protein. This study reported for the first time the BLV genotype 4 in China, and further studies are warranted to compare its immunogenicity and pathogenicity with other BLV genotypes.http://link.springer.com/article/10.1186/s12985-019-1207-8Bovine leukemia virusMolecular characterizationEnvelope geneGenetic diversity |
| spellingShingle | Yi Yang Lina Chen Maoli Dong Wenjiang Huang Xiaoli Hao Yalan Peng Zaicheng Gong Aijian Qin Shaobin Shang Zhangping Yang Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle Virology Journal Bovine leukemia virus Molecular characterization Envelope gene Genetic diversity |
| title | Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle |
| title_full | Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle |
| title_fullStr | Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle |
| title_full_unstemmed | Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle |
| title_short | Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle |
| title_sort | molecular characterization of bovine leukemia virus reveals existence of genotype 4 in chinese dairy cattle |
| topic | Bovine leukemia virus Molecular characterization Envelope gene Genetic diversity |
| url | http://link.springer.com/article/10.1186/s12985-019-1207-8 |
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