Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
Abstract Background Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. Methods We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a vari...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4840-5 |
| _version_ | 1819021920105922560 |
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| author | Kar-Tong Tan Ling-Wen Ding Qiao-Yang Sun Zhen-Tang Lao Wenwen Chien Xi Ren Jin-Fen Xiao Xin Yi Loh Liang Xu Michael Lill Anand Mayakonda De-Chen Lin Henry Yang H. Phillip Koeffler |
| author_facet | Kar-Tong Tan Ling-Wen Ding Qiao-Yang Sun Zhen-Tang Lao Wenwen Chien Xi Ren Jin-Fen Xiao Xin Yi Loh Liang Xu Michael Lill Anand Mayakonda De-Chen Lin Henry Yang H. Phillip Koeffler |
| author_sort | Kar-Tong Tan |
| collection | DOAJ |
| description | Abstract Background Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. Methods We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. Results Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. Conclusions Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines. |
| first_indexed | 2024-12-21T04:14:46Z |
| format | Article |
| id | doaj.art-ab73d11ede72491d9a1d5693f9503c26 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-21T04:14:46Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-ab73d11ede72491d9a1d5693f9503c262022-12-21T19:16:21ZengBMCBMC Cancer1471-24072018-10-0118111310.1186/s12885-018-4840-5Profiling the B/T cell receptor repertoire of lymphocyte derived cell linesKar-Tong Tan0Ling-Wen Ding1Qiao-Yang Sun2Zhen-Tang Lao3Wenwen Chien4Xi Ren5Jin-Fen Xiao6Xin Yi Loh7Liang Xu8Michael Lill9Anand Mayakonda10De-Chen Lin11Henry Yang12H. Phillip Koeffler13Cancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeDivision of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of MedicineCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeDivision of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of MedicineCancer Science Institute of Singapore, National University of SingaporeDivision of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of MedicineCancer Science Institute of Singapore, National University of SingaporeCancer Science Institute of Singapore, National University of SingaporeAbstract Background Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. Methods We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. Results Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. Conclusions Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines.http://link.springer.com/article/10.1186/s12885-018-4840-5BCR/TCR receptor repertoireEBV lymphocytesCancer cell lines |
| spellingShingle | Kar-Tong Tan Ling-Wen Ding Qiao-Yang Sun Zhen-Tang Lao Wenwen Chien Xi Ren Jin-Fen Xiao Xin Yi Loh Liang Xu Michael Lill Anand Mayakonda De-Chen Lin Henry Yang H. Phillip Koeffler Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines BMC Cancer BCR/TCR receptor repertoire EBV lymphocytes Cancer cell lines |
| title | Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines |
| title_full | Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines |
| title_fullStr | Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines |
| title_full_unstemmed | Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines |
| title_short | Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines |
| title_sort | profiling the b t cell receptor repertoire of lymphocyte derived cell lines |
| topic | BCR/TCR receptor repertoire EBV lymphocytes Cancer cell lines |
| url | http://link.springer.com/article/10.1186/s12885-018-4840-5 |
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