Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile
<i>Salmonella</i> Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize <i>S.</i> Infantis strains in poultry production. In this study, 87 <i>S.</i> In...
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MDPI AG
2020-06-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/6/1049 |
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| author | Lisette Lapierre Javiera Cornejo Sebastián Zavala Nicolás Galarce Fernando Sánchez María Belén Benavides Miguel Guzmán Leonardo Sáenz |
| author_facet | Lisette Lapierre Javiera Cornejo Sebastián Zavala Nicolás Galarce Fernando Sánchez María Belén Benavides Miguel Guzmán Leonardo Sáenz |
| author_sort | Lisette Lapierre |
| collection | DOAJ |
| description | <i>Salmonella</i> Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize <i>S.</i> Infantis strains in poultry production. In this study, 87 <i>S.</i> Infantis strains were isolated from chicken meat for sale in supermarkets in Santiago, Chile, and characterized according to their virulence genes, biofilm formation abilities, antibiotic susceptibility, and resistance genes. Through polymerase chain reaction or PCR, the strains were analyzed to detect the presence of 11 virulence genes, 12 antibiotic resistance genes, and integrase genes. Moreover, disc diffusion susceptibility to 18 antimicrobials and the ability to form biofilm in vitro were evaluated. Results demonstrated six different virulence gene profiles. Ninety-four percent of the strains were multi-resistant to antibiotics with weak biofilm formation abilities, 63.2% of the strains were broad spectrum β- lactam resistant, and the <i>bla</i> <sub>CTX-M-65</sub> gene was amplified in 13 strains. Only 3.4% of the strains were fluoroquinolone resistant, and the <i>qnrB</i> gene was amplified in two strains. Colistin resistance was exhibited in 28.7% of the strains, but <i>mrc</i> genes were not amplified in any strain under study. The isolated <i>S.</i> Infantis strains are pathogenic and antibiotic multi-resistant, and thus, this <i>Salmonella</i> serotype should be under surveillance in the poultry food production chain with the aim of protecting public health. |
| first_indexed | 2024-03-10T19:04:25Z |
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| language | English |
| last_indexed | 2024-03-10T19:04:25Z |
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| spelling | doaj.art-ab744ab5a4294cbf8d3b564c73d9b0652023-11-20T04:12:58ZengMDPI AGAnimals2076-26152020-06-01106104910.3390/ani10061049Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in ChileLisette Lapierre0Javiera Cornejo1Sebastián Zavala2Nicolás Galarce3Fernando Sánchez4María Belén Benavides5Miguel Guzmán6Leonardo Sáenz7Department of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileDepartment of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileDepartment of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileDepartment of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileDepartment of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileDepartment of Animal Preventive Medicine, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileLaboratory of Avian Pathology, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8820808, ChileLaboratory of Veterinary Vaccines, Department of Animal Biology, Faculty of Veterinary and Animal Science, Universidad de Chile, Santiago 8820808, Chile<i>Salmonella</i> Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize <i>S.</i> Infantis strains in poultry production. In this study, 87 <i>S.</i> Infantis strains were isolated from chicken meat for sale in supermarkets in Santiago, Chile, and characterized according to their virulence genes, biofilm formation abilities, antibiotic susceptibility, and resistance genes. Through polymerase chain reaction or PCR, the strains were analyzed to detect the presence of 11 virulence genes, 12 antibiotic resistance genes, and integrase genes. Moreover, disc diffusion susceptibility to 18 antimicrobials and the ability to form biofilm in vitro were evaluated. Results demonstrated six different virulence gene profiles. Ninety-four percent of the strains were multi-resistant to antibiotics with weak biofilm formation abilities, 63.2% of the strains were broad spectrum β- lactam resistant, and the <i>bla</i> <sub>CTX-M-65</sub> gene was amplified in 13 strains. Only 3.4% of the strains were fluoroquinolone resistant, and the <i>qnrB</i> gene was amplified in two strains. Colistin resistance was exhibited in 28.7% of the strains, but <i>mrc</i> genes were not amplified in any strain under study. The isolated <i>S.</i> Infantis strains are pathogenic and antibiotic multi-resistant, and thus, this <i>Salmonella</i> serotype should be under surveillance in the poultry food production chain with the aim of protecting public health.https://www.mdpi.com/2076-2615/10/6/1049<i>Salmonella</i> Infantismeat chickenbroilerantimicrobial resistancevirulence genes |
| spellingShingle | Lisette Lapierre Javiera Cornejo Sebastián Zavala Nicolás Galarce Fernando Sánchez María Belén Benavides Miguel Guzmán Leonardo Sáenz Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile Animals <i>Salmonella</i> Infantis meat chicken broiler antimicrobial resistance virulence genes |
| title | Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile |
| title_full | Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile |
| title_fullStr | Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile |
| title_full_unstemmed | Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile |
| title_short | Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in <i>Salmonella</i> Infantis Strains Isolated from Chicken Meat: First Findings in Chile |
| title_sort | phenotypic and genotypic characterization of virulence factors and susceptibility to antibiotics in i salmonella i infantis strains isolated from chicken meat first findings in chile |
| topic | <i>Salmonella</i> Infantis meat chicken broiler antimicrobial resistance virulence genes |
| url | https://www.mdpi.com/2076-2615/10/6/1049 |
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