Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge)
Gibberellic acid (GA) is an important phytohormone that regulates every aspect of plant growth and development. While elements involved in GA signaling have been identified and, hence, their functions have been well studied in model plants, such as Arabidopsis and rice, very little is known in pear....
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Pingli Song Gang Li Jianfeng Xu Qingcui Ma Baoxiu Qi Yuxing Zhang |
| author_facet | Pingli Song Gang Li Jianfeng Xu Qingcui Ma Baoxiu Qi Yuxing Zhang |
| author_sort | Pingli Song |
| collection | DOAJ |
| description | Gibberellic acid (GA) is an important phytohormone that regulates every aspect of plant growth and development. While elements involved in GA signaling have been identified and, hence, their functions have been well studied in model plants, such as Arabidopsis and rice, very little is known in pear. We, therefore, analyzed the genes related to GA signaling from the recently sequenced genome of the wildtype ‘<i>duli</i>’ pear (<i>Pyrus betulifolia</i> Bunge), a widely used rootstock for grafting in pear cultivation in China due to its vigorous growth and resistance to abiotic and biotic stress. In total, 15 genes were identified, including five GA receptors <i>PbGID1s</i> (<i>GA-INSENSTIVE DWARF 1</i>), six GA negative regulators, <i>PbDELLAs</i>, and four GA positive regulators, <i>PbSLYs</i>. Exogenous application of GA could promote the expression of <i>PbGID1s</i> but inhibit that of <i>PbDELLAs</i> and <i>PbSLYs</i> in tissue culture ‘<i>duli</i>’ pear seedlings. The expression profiles of these genes in field-grown trees under normal growth conditions, as well as in tissue-cultured seedlings treated with auxin (IAA), GA, paclobutrazol (PAC), abscisic acid (ABA), and sodium chloride (NaCl), were also studied, providing further evidence of the involvement of these genes in GA signaling in ‘<i>duli</i>’ pear plants. The preliminary results obtained in this report lay a good foundation for future research into GA signaling pathways in pear. Importantly, the identification and preliminary functional verification of these genes could guide molecular breeding in order to obtain the highly desired dwarf pear rootstocks for high-density plantation to aid easy orchard management and high yielding of pear fruits. |
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| spelling | doaj.art-ab782da0d430497bb82bf97a36a081852023-11-23T17:03:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312657010.3390/ijms23126570Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge)Pingli Song0Gang Li1Jianfeng Xu2Qingcui Ma3Baoxiu Qi4Yuxing Zhang5College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaGibberellic acid (GA) is an important phytohormone that regulates every aspect of plant growth and development. While elements involved in GA signaling have been identified and, hence, their functions have been well studied in model plants, such as Arabidopsis and rice, very little is known in pear. We, therefore, analyzed the genes related to GA signaling from the recently sequenced genome of the wildtype ‘<i>duli</i>’ pear (<i>Pyrus betulifolia</i> Bunge), a widely used rootstock for grafting in pear cultivation in China due to its vigorous growth and resistance to abiotic and biotic stress. In total, 15 genes were identified, including five GA receptors <i>PbGID1s</i> (<i>GA-INSENSTIVE DWARF 1</i>), six GA negative regulators, <i>PbDELLAs</i>, and four GA positive regulators, <i>PbSLYs</i>. Exogenous application of GA could promote the expression of <i>PbGID1s</i> but inhibit that of <i>PbDELLAs</i> and <i>PbSLYs</i> in tissue culture ‘<i>duli</i>’ pear seedlings. The expression profiles of these genes in field-grown trees under normal growth conditions, as well as in tissue-cultured seedlings treated with auxin (IAA), GA, paclobutrazol (PAC), abscisic acid (ABA), and sodium chloride (NaCl), were also studied, providing further evidence of the involvement of these genes in GA signaling in ‘<i>duli</i>’ pear plants. The preliminary results obtained in this report lay a good foundation for future research into GA signaling pathways in pear. Importantly, the identification and preliminary functional verification of these genes could guide molecular breeding in order to obtain the highly desired dwarf pear rootstocks for high-density plantation to aid easy orchard management and high yielding of pear fruits.https://www.mdpi.com/1422-0067/23/12/6570‘<i>duli</i>’ pear<i>Pyrus betulifolia</i> BungeGA signalingGID1DELLASLY |
| spellingShingle | Pingli Song Gang Li Jianfeng Xu Qingcui Ma Baoxiu Qi Yuxing Zhang Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) International Journal of Molecular Sciences ‘<i>duli</i>’ pear <i>Pyrus betulifolia</i> Bunge GA signaling GID1 DELLA SLY |
| title | Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) |
| title_full | Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) |
| title_fullStr | Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) |
| title_full_unstemmed | Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) |
| title_short | Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘<i>duli</i>’ Pear (<i>Pyrus betulifolia</i> Bunge) |
| title_sort | genome wide analysis of genes involved in the ga signal transduction pathway in i duli i pear i pyrus betulifolia i bunge |
| topic | ‘<i>duli</i>’ pear <i>Pyrus betulifolia</i> Bunge GA signaling GID1 DELLA SLY |
| url | https://www.mdpi.com/1422-0067/23/12/6570 |
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