Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis
Objectives: The aim of this study was to examine how the concentrated delivery of less effective antibiotics, such as the β-lactam penicillin G, by linkage to nanoparticles (NPs), could influence the killing efficiency against various pathogenic bacteria, including methicillin-resistant Staphylococc...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520301685 |
| _version_ | 1819062954269605888 |
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| author | Amjed Alabresm Yung Pin Chen Savannah Wichter-Chandler Jamie Lead Brian C. Benicewicz Alan W. Decho |
| author_facet | Amjed Alabresm Yung Pin Chen Savannah Wichter-Chandler Jamie Lead Brian C. Benicewicz Alan W. Decho |
| author_sort | Amjed Alabresm |
| collection | DOAJ |
| description | Objectives: The aim of this study was to examine how the concentrated delivery of less effective antibiotics, such as the β-lactam penicillin G, by linkage to nanoparticles (NPs), could influence the killing efficiency against various pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and other multidrug resistant (MDR) strains. Methods: The β-lactam antibiotic penicillin G (PenG) was passively sorbed to fluorescent polystyrene NPs (20 nm) that were surface-functionalized with carboxylic acid (COO−-NPs) or sulfate groups (SO4−-NPs) to form a PenG-NP complex. Antimicrobial activities of PenG-NPs were evaluated against Gram-negative and Gram-positive bacteria, including antibiotic resistant strains. Disc diffusion, microdilution assays and live/dead staining were performed for antibacterial assessments. Results: The results showed that bactericidal activities of PenG-NP complexes were statistically significantly (P < 0.05) enhanced against Gram-negative and Gram-positive strains, including MRSA and MDR strains. Fluorescence imaging verified that NPs comigrated with antibiotics throughout clear zones of MIC agar plate assays. The increased bactericidal abilities of NP-linked antibiotics are hypothesized to result from the greatly increased densities of antibiotic delivered by each NP to a given bacterial cell (compared with solution concentrations of antibiotic), which overwhelms the bacterial resistance mechanism(s). Conclusions: As a whole, PenG-NP complexation demonstrated a remarkable activity against different pathogenic bacteria, including MRSA and MDR strains. We term this the ‘grenade hypothesis’. Further testing and development of this approach will provide validation of its potential usefulness for controlling antibiotic-resistant bacterial infections. |
| first_indexed | 2024-12-21T15:06:59Z |
| format | Article |
| id | doaj.art-ab79910c96ce4fdaa66a0a86dfd7f47a |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-21T15:06:59Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-ab79910c96ce4fdaa66a0a86dfd7f47a2022-12-21T18:59:25ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-09-0122811817Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesisAmjed Alabresm0Yung Pin Chen1Savannah Wichter-Chandler2Jamie Lead3Brian C. Benicewicz4Alan W. Decho5Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States; Center for Environmental Nanoscience and Risk (CENR), University of South Carolina, Columbia, SC, United States; Department of Biological Development of Shatt Al-Arab & N. Arabian Gulf, Marine Science Centre, University of Basrah, Basrah, IraqDepartment of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United StatesDepartment of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United StatesDepartment of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States; Center for Environmental Nanoscience and Risk (CENR), University of South Carolina, Columbia, SC, United StatesDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, United StatesDepartment of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States; Corresponding author at: Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States.Objectives: The aim of this study was to examine how the concentrated delivery of less effective antibiotics, such as the β-lactam penicillin G, by linkage to nanoparticles (NPs), could influence the killing efficiency against various pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and other multidrug resistant (MDR) strains. Methods: The β-lactam antibiotic penicillin G (PenG) was passively sorbed to fluorescent polystyrene NPs (20 nm) that were surface-functionalized with carboxylic acid (COO−-NPs) or sulfate groups (SO4−-NPs) to form a PenG-NP complex. Antimicrobial activities of PenG-NPs were evaluated against Gram-negative and Gram-positive bacteria, including antibiotic resistant strains. Disc diffusion, microdilution assays and live/dead staining were performed for antibacterial assessments. Results: The results showed that bactericidal activities of PenG-NP complexes were statistically significantly (P < 0.05) enhanced against Gram-negative and Gram-positive strains, including MRSA and MDR strains. Fluorescence imaging verified that NPs comigrated with antibiotics throughout clear zones of MIC agar plate assays. The increased bactericidal abilities of NP-linked antibiotics are hypothesized to result from the greatly increased densities of antibiotic delivered by each NP to a given bacterial cell (compared with solution concentrations of antibiotic), which overwhelms the bacterial resistance mechanism(s). Conclusions: As a whole, PenG-NP complexation demonstrated a remarkable activity against different pathogenic bacteria, including MRSA and MDR strains. We term this the ‘grenade hypothesis’. Further testing and development of this approach will provide validation of its potential usefulness for controlling antibiotic-resistant bacterial infections.http://www.sciencedirect.com/science/article/pii/S2213716520301685AntibioticNanoparticleAntibiotic-resistancePenicillin-GMRSAMDR |
| spellingShingle | Amjed Alabresm Yung Pin Chen Savannah Wichter-Chandler Jamie Lead Brian C. Benicewicz Alan W. Decho Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis Journal of Global Antimicrobial Resistance Antibiotic Nanoparticle Antibiotic-resistance Penicillin-G MRSA MDR |
| title | Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis |
| title_full | Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis |
| title_fullStr | Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis |
| title_full_unstemmed | Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis |
| title_short | Nanoparticles as antibiotic-delivery vehicles (ADVs) overcome resistance by MRSA and other MDR bacterial pathogens: The grenade hypothesis |
| title_sort | nanoparticles as antibiotic delivery vehicles advs overcome resistance by mrsa and other mdr bacterial pathogens the grenade hypothesis |
| topic | Antibiotic Nanoparticle Antibiotic-resistance Penicillin-G MRSA MDR |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520301685 |
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