MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model
<i>Vibrio vulnificus</i> causes rapid septicemia in susceptible individuals who have ingested contaminated foods or have open wounds exposed to seawater contaminated with the bacteria. Despite antibiotic therapy and aggressive debridement, mortality from septicemia is high. In this study...
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/934 |
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| author | Takashige Kashimoto Kohei Yamazaki Takehiro Kado Kaho Matsuda Shunji Ueno |
| author_facet | Takashige Kashimoto Kohei Yamazaki Takehiro Kado Kaho Matsuda Shunji Ueno |
| author_sort | Takashige Kashimoto |
| collection | DOAJ |
| description | <i>Vibrio vulnificus</i> causes rapid septicemia in susceptible individuals who have ingested contaminated foods or have open wounds exposed to seawater contaminated with the bacteria. Despite antibiotic therapy and aggressive debridement, mortality from septicemia is high. In this study, we showed that MukB mutation (<i>mukB</i>::Tn) affected the proliferation of <i>V. vulnificus</i> in the systemic circulation but not at the inoculation site in the wound infection model. A comparison of <i>mukB</i>::Tn with WT and a <i>mukB</i> complement strain (<i>mukB</i>::Tn/p<i>mukB</i>) on the bacterial burden in the muscle at the infection site showed that spreading and proliferation of the <i>mukB</i>::Tn strain was similar to those of the other strains. However, the bacterial burden of <i>mukB</i>::Tn in the spleen was reduced compared to that of the WT strain in the wound infection model. In a competition experiment, we found a lower bacterial burden of <i>mukB</i>::Tn in the spleen than that of the WT strain infecting the systemic circulation. Here, we report on a gene required for the rapid proliferation of <i>V. vulnificus</i> only in the systemic circulation and potentially required for its survival. Our finding may provide a novel therapeutic target for <i>V. vulnificus</i> septicemia. |
| first_indexed | 2024-03-10T11:56:12Z |
| format | Article |
| id | doaj.art-ab7b952fe7a849b79b724b783b0c8c58 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:56:12Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ab7b952fe7a849b79b724b783b0c8c582023-11-21T17:20:29ZengMDPI AGMicroorganisms2076-26072021-04-019593410.3390/microorganisms9050934MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection ModelTakashige Kashimoto0Kohei Yamazaki1Takehiro Kado2Kaho Matsuda3Shunji Ueno4Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, JapanLaboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, JapanLaboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, JapanLaboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, JapanLaboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, Japan<i>Vibrio vulnificus</i> causes rapid septicemia in susceptible individuals who have ingested contaminated foods or have open wounds exposed to seawater contaminated with the bacteria. Despite antibiotic therapy and aggressive debridement, mortality from septicemia is high. In this study, we showed that MukB mutation (<i>mukB</i>::Tn) affected the proliferation of <i>V. vulnificus</i> in the systemic circulation but not at the inoculation site in the wound infection model. A comparison of <i>mukB</i>::Tn with WT and a <i>mukB</i> complement strain (<i>mukB</i>::Tn/p<i>mukB</i>) on the bacterial burden in the muscle at the infection site showed that spreading and proliferation of the <i>mukB</i>::Tn strain was similar to those of the other strains. However, the bacterial burden of <i>mukB</i>::Tn in the spleen was reduced compared to that of the WT strain in the wound infection model. In a competition experiment, we found a lower bacterial burden of <i>mukB</i>::Tn in the spleen than that of the WT strain infecting the systemic circulation. Here, we report on a gene required for the rapid proliferation of <i>V. vulnificus</i> only in the systemic circulation and potentially required for its survival. Our finding may provide a novel therapeutic target for <i>V. vulnificus</i> septicemia.https://www.mdpi.com/2076-2607/9/5/934<i>Vibrio vulnificus</i>sepsisMukB |
| spellingShingle | Takashige Kashimoto Kohei Yamazaki Takehiro Kado Kaho Matsuda Shunji Ueno MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model Microorganisms <i>Vibrio vulnificus</i> sepsis MukB |
| title | MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model |
| title_full | MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model |
| title_fullStr | MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model |
| title_full_unstemmed | MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model |
| title_short | MukB Is a Gene Necessary for Rapid Proliferation of <i>Vibrio vulnificus</i> in the Systemic Circulation but Not at the Local Infection Site in the Mouse Wound Infection Model |
| title_sort | mukb is a gene necessary for rapid proliferation of i vibrio vulnificus i in the systemic circulation but not at the local infection site in the mouse wound infection model |
| topic | <i>Vibrio vulnificus</i> sepsis MukB |
| url | https://www.mdpi.com/2076-2607/9/5/934 |
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