Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City
Hospitals are hotspots for antimicrobial-resistant bacteria and will be expelled from hospitals via wastewater systems. In this study, we present ESBLs prevalence in Entrobacteriaceae strains isolated from hospitals and urban wastewaters in Karaj.A total of 100 Entrobacteriaceae strains were isolate...
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| Format: | Article |
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Water and Wastewater Consulting Engineers Research Development
2020-07-01
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| Series: | آب و فاضلاب |
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| Online Access: | http://www.wwjournal.ir/article_99119_4a164d8f47904e8773227b5f9b3a1a27.pdf |
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| author | Maryam Afshari Azam Haddadi Mahmoud Shavandi |
| author_facet | Maryam Afshari Azam Haddadi Mahmoud Shavandi |
| author_sort | Maryam Afshari |
| collection | DOAJ |
| description | Hospitals are hotspots for antimicrobial-resistant bacteria and will be expelled from hospitals via wastewater systems. In this study, we present ESBLs prevalence in Entrobacteriaceae strains isolated from hospitals and urban wastewaters in Karaj.A total of 100 Entrobacteriaceae strains were isolated from hospitals and urban wastewater in Alborz province during the summer of 2018. Bacterial strains were identified by standard microbiological and biochemical tests. The antimicrobial susceptibility test was determined according to Kirby Baur assay. ESBLs producers were screened by disc diffusion and combined disc and were studied by using specific primers for blaTEM and blaSHV genes. Among the organisms cultured, Escherichia coli (73%) was the most common organism followed by Klebsiella oxytoka (7%). Antibiotic resistance patterns were observed as follows: cefotaxim 26%, ceftriaxone 21%, ceftazidim 15% and cefepim 9%. Thirteen percent of isolates were detected as ESBLs producers. Based on the PCR results the frequency of blaTEM and blaSHV among the ESBLs producer isolates were 92.3% and 100% respectively. Antimicrobial resistance should now be seen as an environmental pollutant and new wastewater treatment processes must be assessed for their capability in eliminating antimicrobial-resistant bacteria, especially from hospital effluents. |
| first_indexed | 2024-12-18T23:23:15Z |
| format | Article |
| id | doaj.art-ab7e8e212c974a82824ed2d1bf274ced |
| institution | Directory Open Access Journal |
| issn | 1024-5936 2383-0905 |
| language | English |
| last_indexed | 2024-12-18T23:23:15Z |
| publishDate | 2020-07-01 |
| publisher | Water and Wastewater Consulting Engineers Research Development |
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| series | آب و فاضلاب |
| spelling | doaj.art-ab7e8e212c974a82824ed2d1bf274ced2022-12-21T20:47:53ZengWater and Wastewater Consulting Engineers Research Developmentآب و فاضلاب1024-59362383-09052020-07-01313687610.22093/wwj.2019.174603.284199119Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj CityMaryam Afshari0Azam Haddadi1Mahmoud Shavandi2Former Graduate Student of Microbiology, Dept. of Microbiology, Karaj Branch, Islamic Azad University, Karaj, IranAssist. Prof., Dept. of Microbiology, Karaj Branch, Islamic Azad University, Karaj, IranAssist. Prof., Ecology and Environmental Pollution Control Research Group, Research Institute of Petroleum Industry, Tehran, IranHospitals are hotspots for antimicrobial-resistant bacteria and will be expelled from hospitals via wastewater systems. In this study, we present ESBLs prevalence in Entrobacteriaceae strains isolated from hospitals and urban wastewaters in Karaj.A total of 100 Entrobacteriaceae strains were isolated from hospitals and urban wastewater in Alborz province during the summer of 2018. Bacterial strains were identified by standard microbiological and biochemical tests. The antimicrobial susceptibility test was determined according to Kirby Baur assay. ESBLs producers were screened by disc diffusion and combined disc and were studied by using specific primers for blaTEM and blaSHV genes. Among the organisms cultured, Escherichia coli (73%) was the most common organism followed by Klebsiella oxytoka (7%). Antibiotic resistance patterns were observed as follows: cefotaxim 26%, ceftriaxone 21%, ceftazidim 15% and cefepim 9%. Thirteen percent of isolates were detected as ESBLs producers. Based on the PCR results the frequency of blaTEM and blaSHV among the ESBLs producer isolates were 92.3% and 100% respectively. Antimicrobial resistance should now be seen as an environmental pollutant and new wastewater treatment processes must be assessed for their capability in eliminating antimicrobial-resistant bacteria, especially from hospital effluents.http://www.wwjournal.ir/article_99119_4a164d8f47904e8773227b5f9b3a1a27.pdfesblentrobacteriaceaewastewaterantibiotic resistance |
| spellingShingle | Maryam Afshari Azam Haddadi Mahmoud Shavandi Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City آب و فاضلاب esbl entrobacteriaceae wastewater antibiotic resistance |
| title | Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City |
| title_full | Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City |
| title_fullStr | Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City |
| title_full_unstemmed | Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City |
| title_short | Prevalence Assay of Antibiotic Resistance in Enterobacteriaceae Isolated from Hospital and Urban Wastewaters in Karaj City |
| title_sort | prevalence assay of antibiotic resistance in enterobacteriaceae isolated from hospital and urban wastewaters in karaj city |
| topic | esbl entrobacteriaceae wastewater antibiotic resistance |
| url | http://www.wwjournal.ir/article_99119_4a164d8f47904e8773227b5f9b3a1a27.pdf |
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