Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum
The basal eudicot Epimedium (Barrenwort) exhibits innovative floral morphology in the form of petal spurs filled with nectar and petaloid sepals. The B-class MADS-box genes APETALAS3 (AP3) and PISTILLA (PI) determine sepal and petal identity while the C-class gene AGAMOUS (AG) determines carpel and...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2023-02-01
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| Series: | Medicinal Plant Biology |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/MPB-2023-0001 |
| _version_ | 1797279340659998720 |
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| author | Wei Sun Huihua Wan Wenjun Huang Zubaida Yousaf Hongwen Huang Wang Ying |
| author_facet | Wei Sun Huihua Wan Wenjun Huang Zubaida Yousaf Hongwen Huang Wang Ying |
| author_sort | Wei Sun |
| collection | DOAJ |
| description | The basal eudicot Epimedium (Barrenwort) exhibits innovative floral morphology in the form of petal spurs filled with nectar and petaloid sepals. The B-class MADS-box genes APETALAS3 (AP3) and PISTILLA (PI) determine sepal and petal identity while the C-class gene AGAMOUS (AG) determines carpel and stamen identity in Arabidopsis. Complex histories of gene duplication resulted in subsequent subfunctionalization or neofunctionalization of paralogs. Here, a total of four B- and two C-class genes were successfully isolated from E. sagittatum. Phylogenetic analysis showed that EsAP3-1, EsAP3-2, and EsAP3-3 are part of the AP3 clade; EsPI is part of the PI group; and EsAG and EsAG11 clustered into the AG and AG11 groups, respectively. Quantitative real-time PCR was utilized to detect the expression patterns of these genes, and all B-class genes except for EsAP3-3 were found to be universally expressed. The transcribed EsAG and EsAG11 genes were confined to reproductive organs. In addition, yeast three and two hybrid assays were used to explore the status of protein complexes. EsAP3-1 was found to have broadly interactive partners, and EsPI can form a heterodimer with EsAP3-1 and EsAP3-2. Transgenic EsAG overexpression in wild-type Arabidopsis confirmed the conserved function determining carpel development. |
| first_indexed | 2024-03-07T16:23:53Z |
| format | Article |
| id | doaj.art-ab840385c459400da72891eeeb4ca8fc |
| institution | Directory Open Access Journal |
| issn | 2835-6969 |
| language | English |
| last_indexed | 2024-03-07T16:23:53Z |
| publishDate | 2023-02-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Medicinal Plant Biology |
| spelling | doaj.art-ab840385c459400da72891eeeb4ca8fc2024-03-04T01:28:11ZengMaximum Academic PressMedicinal Plant Biology2835-69692023-02-01211710.48130/MPB-2023-0001MPB-2023-0001Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatumWei Sun0Huihua Wan1Wenjun Huang2Zubaida Yousaf3Hongwen Huang4Wang Ying5Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100070, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100070, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, ChinaDepartment of Botany, Lahore College for Women University, Lahore, PakistanLushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Center of Economic Botany/Core Botanical Gardens, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaThe basal eudicot Epimedium (Barrenwort) exhibits innovative floral morphology in the form of petal spurs filled with nectar and petaloid sepals. The B-class MADS-box genes APETALAS3 (AP3) and PISTILLA (PI) determine sepal and petal identity while the C-class gene AGAMOUS (AG) determines carpel and stamen identity in Arabidopsis. Complex histories of gene duplication resulted in subsequent subfunctionalization or neofunctionalization of paralogs. Here, a total of four B- and two C-class genes were successfully isolated from E. sagittatum. Phylogenetic analysis showed that EsAP3-1, EsAP3-2, and EsAP3-3 are part of the AP3 clade; EsPI is part of the PI group; and EsAG and EsAG11 clustered into the AG and AG11 groups, respectively. Quantitative real-time PCR was utilized to detect the expression patterns of these genes, and all B-class genes except for EsAP3-3 were found to be universally expressed. The transcribed EsAG and EsAG11 genes were confined to reproductive organs. In addition, yeast three and two hybrid assays were used to explore the status of protein complexes. EsAP3-1 was found to have broadly interactive partners, and EsPI can form a heterodimer with EsAP3-1 and EsAP3-2. Transgenic EsAG overexpression in wild-type Arabidopsis confirmed the conserved function determining carpel development.https://www.maxapress.com/article/doi/10.48130/MPB-2023-0001epimediummads-boxap3/pimedicinalplant |
| spellingShingle | Wei Sun Huihua Wan Wenjun Huang Zubaida Yousaf Hongwen Huang Wang Ying Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum Medicinal Plant Biology epimedium mads-box ap3/pi medicinal plant |
| title | Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum |
| title_full | Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum |
| title_fullStr | Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum |
| title_full_unstemmed | Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum |
| title_short | Characterization of B- and C-class MADS-box genes in medicinal plant Epimedium sagittatum |
| title_sort | characterization of b and c class mads box genes in medicinal plant epimedium sagittatum |
| topic | epimedium mads-box ap3/pi medicinal plant |
| url | https://www.maxapress.com/article/doi/10.48130/MPB-2023-0001 |
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