Modern Methods of DNA Sequencing (Scientific Review)
Rapidly developing sequencing technologies have provided the possibility for identification of a DNA nucleotide sequence of a whole individual human genome just in a couple of weeks. Working capacity of some sequencers is already measured in thousands of milliards of base pairs per an operating cycl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2014-06-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/143 |
| _version_ | 1797220889669926912 |
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| author | Ya. M. Krasnov N. P. Guseva N. A. Sharapova A. V. Cherkasov |
| author_facet | Ya. M. Krasnov N. P. Guseva N. A. Sharapova A. V. Cherkasov |
| author_sort | Ya. M. Krasnov |
| collection | DOAJ |
| description | Rapidly developing sequencing technologies have provided the possibility for identification of a DNA nucleotide sequence of a whole individual human genome just in a couple of weeks. Working capacity of some sequencers is already measured in thousands of milliards of base pairs per an operating cycle. Reviewed have been the basic principles and analytical potential of the modern methods for DNA sequencing which are nominally subdivided into three major types: classical - capillary electrophoresis sequencing and pyrosequencing, novel (Next Generation Sequencing - NGS) - simultaneous sequencing of millions of DNA fragments, each one of which is a cluster containing many thousands or hundreds of thousands of their clones - high-performance pyrosequencing, cyclic ligase and semiconducting sequencing, molecular-cluster-based sequencing using fluorescent-labeled precursors; and cutting-edge methods - (Next-Next Generation Sequencing - NNGS) - the ones that read millions of single DNA fragments without pre-cloning. |
| first_indexed | 2024-03-08T13:43:40Z |
| format | Article |
| id | doaj.art-ab84fe606ac94ead8fab9d4d2c79b541 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:43Z |
| publishDate | 2014-06-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-ab84fe606ac94ead8fab9d4d2c79b5412024-04-05T16:47:10ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-06-0102737910.21055/0370-1069-2014-2-73-79143Modern Methods of DNA Sequencing (Scientific Review)Ya. M. Krasnov0N. P. Guseva1N. A. Sharapova2A. V. Cherkasov3Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Rapidly developing sequencing technologies have provided the possibility for identification of a DNA nucleotide sequence of a whole individual human genome just in a couple of weeks. Working capacity of some sequencers is already measured in thousands of milliards of base pairs per an operating cycle. Reviewed have been the basic principles and analytical potential of the modern methods for DNA sequencing which are nominally subdivided into three major types: classical - capillary electrophoresis sequencing and pyrosequencing, novel (Next Generation Sequencing - NGS) - simultaneous sequencing of millions of DNA fragments, each one of which is a cluster containing many thousands or hundreds of thousands of their clones - high-performance pyrosequencing, cyclic ligase and semiconducting sequencing, molecular-cluster-based sequencing using fluorescent-labeled precursors; and cutting-edge methods - (Next-Next Generation Sequencing - NNGS) - the ones that read millions of single DNA fragments without pre-cloning.https://journal.microbe.ru/jour/article/view/143секвенированиеметодыднкsequencingmethodsdna |
| spellingShingle | Ya. M. Krasnov N. P. Guseva N. A. Sharapova A. V. Cherkasov Modern Methods of DNA Sequencing (Scientific Review) Проблемы особо опасных инфекций секвенирование методы днк sequencing methods dna |
| title | Modern Methods of DNA Sequencing (Scientific Review) |
| title_full | Modern Methods of DNA Sequencing (Scientific Review) |
| title_fullStr | Modern Methods of DNA Sequencing (Scientific Review) |
| title_full_unstemmed | Modern Methods of DNA Sequencing (Scientific Review) |
| title_short | Modern Methods of DNA Sequencing (Scientific Review) |
| title_sort | modern methods of dna sequencing scientific review |
| topic | секвенирование методы днк sequencing methods dna |
| url | https://journal.microbe.ru/jour/article/view/143 |
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