Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption
The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial i...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X21001016 |
| _version_ | 1819099846802407424 |
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| author | Jessica M Bedoya Vélez José Gregorio Martínez Juliana Tobón Ospina Susana Ochoa Agudelo |
| author_facet | Jessica M Bedoya Vélez José Gregorio Martínez Juliana Tobón Ospina Susana Ochoa Agudelo |
| author_sort | Jessica M Bedoya Vélez |
| collection | DOAJ |
| description | The mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial isolates were obtained from wastewater bodies from an industrial zone in the municipality of Girardota, Antioquia, Colombia. Subsequently, they were selected to evaluate their lead tolerance capacity at different concentrations. In addition, some parameters were determined, such as the capacity to produce exopolysaccharides and their biosorption to understand potential mechanisms associated to lead tolerance. According to the biocehemical test (Vitek) and the molecular analysis of sequences of 16S rDNA, bacterial were identified as Pseudomonas aeruginosa, Pseudomonas nitroreducens, and Pseudomonas alcaligenes. We determined that the seven isolates had the capacity to tolerate concentrations higher than 50 mg/ml of lead, and that the concentration and exposure time (40 h) to this metal significantly affect the Pseudomonas spp. isolates. Statistically significant differences were detected (p < 0.05) in the production of the exopolysaccharide (EPS) among the isolates. P. aeruginosa (P16) was the strain with the maximum absorbance exopolysaccharide measured. We evidenced that P. aeruginosa (P14) and P. nitroreducens (P20) have 80% capacity to biosorber lead using live mass (minimum range from 80.9% to 87%). It is suggested that the tolerance to lead exhibited by the environmental isolates of Pseudomonas spp. can be attributed to the production of exopolysaccharides and biosorption, which are protection factors for its survival in contaminated places. Finally, it was determined that the adsorption measured from dead biomass was significant (p < 0.05) from 40 h of exposure to metal (Average 182.2 ± 7). We generated new knowledge about the potential use of the Pseudomonas spp. genus to bioremediate affluent contaminated with heavy metals. |
| first_indexed | 2024-12-22T00:53:22Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-22T00:53:22Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-ab88d3f0f37f4bf68138354993554b0a2022-12-21T18:44:22ZengElsevierBiotechnology Reports2215-017X2021-12-0132e00685Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorptionJessica M Bedoya Vélez0José Gregorio Martínez1Juliana Tobón Ospina2Susana Ochoa Agudelo3Grupo de investigación Biociencias, Facultad Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia (IUCMA), Street 78 # 65 - 46 Patrimonial Campus, Medellín 050003, ColombiaGrupo de investigación Biociencias, Facultad Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia (IUCMA), Street 78 # 65 - 46 Patrimonial Campus, Medellín 050003, ColombiaGrupo de investigación Biociencias, Facultad Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia (IUCMA), Street 78 # 65 - 46 Patrimonial Campus, Medellín 050003, ColombiaCorresponding author at: https://orcid.org/0000-0001-5369-7137.; Grupo de investigación Biociencias, Facultad Ciencias de la Salud, Institución Universitaria Colegio Mayor de Antioquia (IUCMA), Street 78 # 65 - 46 Patrimonial Campus, Medellín 050003, ColombiaThe mechanisms of tolerance to heavy metals used by some microorganisms identified by bioprospection processes are useful for the development and implementation of bioremediation strategies for contaminated environments with high toxic load caused by heavy metals. A total of seven native microbial isolates were obtained from wastewater bodies from an industrial zone in the municipality of Girardota, Antioquia, Colombia. Subsequently, they were selected to evaluate their lead tolerance capacity at different concentrations. In addition, some parameters were determined, such as the capacity to produce exopolysaccharides and their biosorption to understand potential mechanisms associated to lead tolerance. According to the biocehemical test (Vitek) and the molecular analysis of sequences of 16S rDNA, bacterial were identified as Pseudomonas aeruginosa, Pseudomonas nitroreducens, and Pseudomonas alcaligenes. We determined that the seven isolates had the capacity to tolerate concentrations higher than 50 mg/ml of lead, and that the concentration and exposure time (40 h) to this metal significantly affect the Pseudomonas spp. isolates. Statistically significant differences were detected (p < 0.05) in the production of the exopolysaccharide (EPS) among the isolates. P. aeruginosa (P16) was the strain with the maximum absorbance exopolysaccharide measured. We evidenced that P. aeruginosa (P14) and P. nitroreducens (P20) have 80% capacity to biosorber lead using live mass (minimum range from 80.9% to 87%). It is suggested that the tolerance to lead exhibited by the environmental isolates of Pseudomonas spp. can be attributed to the production of exopolysaccharides and biosorption, which are protection factors for its survival in contaminated places. Finally, it was determined that the adsorption measured from dead biomass was significant (p < 0.05) from 40 h of exposure to metal (Average 182.2 ± 7). We generated new knowledge about the potential use of the Pseudomonas spp. genus to bioremediate affluent contaminated with heavy metals.http://www.sciencedirect.com/science/article/pii/S2215017X21001016BioprospectingHeavy metalsToxicBioaccumulationLive biomassDead biomass |
| spellingShingle | Jessica M Bedoya Vélez José Gregorio Martínez Juliana Tobón Ospina Susana Ochoa Agudelo Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption Biotechnology Reports Bioprospecting Heavy metals Toxic Bioaccumulation Live biomass Dead biomass |
| title | Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| title_full | Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| title_fullStr | Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| title_full_unstemmed | Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| title_short | Bioremediation potential of Pseudomonas genus isolates from residual water, capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| title_sort | bioremediation potential of pseudomonas genus isolates from residual water capable of tolerating lead through mechanisms of exopolysaccharide production and biosorption |
| topic | Bioprospecting Heavy metals Toxic Bioaccumulation Live biomass Dead biomass |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X21001016 |
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