Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study
Chronic oxidative stress (OS) can be an important factor of acute myeloid leukemia (AML) progression; however, there are no data on the extent/consequence of OS after transfusion of packed red blood cells (pRBCs) and platelet concentrates (PCs), which are commonly used in the treatment of leukemia-a...
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MDPI AG
2021-03-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/7/1349 |
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| author | Kamila Czubak-Prowizor Jacek Trelinski Paulina Stelmach Piotr Stelmach Agnieszka Madon Halina Malgorzata Zbikowska |
| author_facet | Kamila Czubak-Prowizor Jacek Trelinski Paulina Stelmach Piotr Stelmach Agnieszka Madon Halina Malgorzata Zbikowska |
| author_sort | Kamila Czubak-Prowizor |
| collection | DOAJ |
| description | Chronic oxidative stress (OS) can be an important factor of acute myeloid leukemia (AML) progression; however, there are no data on the extent/consequence of OS after transfusion of packed red blood cells (pRBCs) and platelet concentrates (PCs), which are commonly used in the treatment of leukemia-associated anemia and thrombocytopenia. We aimed to investigate the effects of pRBC/PC transfusion on the OS markers, i.e., thiol and carbonyl (CO) groups, 3-nitrotyrosine (3-NT), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGE), total antioxidant capacity (TAC), SOD, GST, and LDH, in the blood plasma of AML patients, before and 24 h post-transfusion. In this exploratory study, 52 patients were examined, of which 27 were transfused with pRBCs and 25 with PCs. Age-matched healthy subjects were also enrolled as controls. Our results showed the oxidation of thiols, increased 3-NT, AGE levels, and decreased TAC in AML groups versus controls. After pRBC transfusion, CO groups, AGE, and 3-NT significantly increased (by approximately 30, 23, and 35%; <i>p</i> < 0.05, <i>p</i> < 0.05, and <i>p</i> < 0.01, respectively) while thiols reduced (by 18%; <i>p</i> < 0.05). The PC transfusion resulted in the raise of TBARS and AGE (by 45%; <i>p</i> < 0.01 and 31%; <i>p</i> < 0.001), respectively). Other variables showed no significant post-transfusion changes. In conclusion, transfusion of both pRBCs and PCs was associated with an increased OS; however, transfusing the former may have more severe consequences, since it is associated with the irreversible oxidative/nitrative modifications of plasma proteins. |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T12:56:22Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-ab8aa27133994af1a5d125030348cd0d2023-11-21T11:55:24ZengMDPI AGJournal of Clinical Medicine2077-03832021-03-01107134910.3390/jcm10071349Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory StudyKamila Czubak-Prowizor0Jacek Trelinski1Paulina Stelmach2Piotr Stelmach3Agnieszka Madon4Halina Malgorzata Zbikowska5Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, PolandDepartment of Coagulation Disorders, Medical University of Lodz, Ciolkowskiego 2, 93-510 Lodz, PolandDepartment of Haematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2, 93-510 Lodz, PolandDepartment of Haematology, Medical University of Lodz, Copernicus Memorial Hospital, Ciolkowskiego 2, 93-510 Lodz, PolandLaboratory of Transfusion Serology and Blood Bank, Copernicus Memorial Hospital, Pabianicka 62, 93-513 Lodz, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, PolandChronic oxidative stress (OS) can be an important factor of acute myeloid leukemia (AML) progression; however, there are no data on the extent/consequence of OS after transfusion of packed red blood cells (pRBCs) and platelet concentrates (PCs), which are commonly used in the treatment of leukemia-associated anemia and thrombocytopenia. We aimed to investigate the effects of pRBC/PC transfusion on the OS markers, i.e., thiol and carbonyl (CO) groups, 3-nitrotyrosine (3-NT), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGE), total antioxidant capacity (TAC), SOD, GST, and LDH, in the blood plasma of AML patients, before and 24 h post-transfusion. In this exploratory study, 52 patients were examined, of which 27 were transfused with pRBCs and 25 with PCs. Age-matched healthy subjects were also enrolled as controls. Our results showed the oxidation of thiols, increased 3-NT, AGE levels, and decreased TAC in AML groups versus controls. After pRBC transfusion, CO groups, AGE, and 3-NT significantly increased (by approximately 30, 23, and 35%; <i>p</i> < 0.05, <i>p</i> < 0.05, and <i>p</i> < 0.01, respectively) while thiols reduced (by 18%; <i>p</i> < 0.05). The PC transfusion resulted in the raise of TBARS and AGE (by 45%; <i>p</i> < 0.01 and 31%; <i>p</i> < 0.001), respectively). Other variables showed no significant post-transfusion changes. In conclusion, transfusion of both pRBCs and PCs was associated with an increased OS; however, transfusing the former may have more severe consequences, since it is associated with the irreversible oxidative/nitrative modifications of plasma proteins.https://www.mdpi.com/2077-0383/10/7/1349acute myeloid leukemiablood plateletred blood celltransfusionoxidative stress markers |
| spellingShingle | Kamila Czubak-Prowizor Jacek Trelinski Paulina Stelmach Piotr Stelmach Agnieszka Madon Halina Malgorzata Zbikowska Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study Journal of Clinical Medicine acute myeloid leukemia blood platelet red blood cell transfusion oxidative stress markers |
| title | Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study |
| title_full | Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study |
| title_fullStr | Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study |
| title_full_unstemmed | Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study |
| title_short | Increased Oxidative Stress in Acute Myeloid Leukemia Patients after Red Blood Cell Transfusion, but Not Platelet Transfusion, Results Mainly from the Oxidative/Nitrative Protein Damage: An Exploratory Study |
| title_sort | increased oxidative stress in acute myeloid leukemia patients after red blood cell transfusion but not platelet transfusion results mainly from the oxidative nitrative protein damage an exploratory study |
| topic | acute myeloid leukemia blood platelet red blood cell transfusion oxidative stress markers |
| url | https://www.mdpi.com/2077-0383/10/7/1349 |
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