Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil
BACKGROUND Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis, and the number of new cases of multidrug resistant TB (MDR-TB), pre extensively drug-resistant TB (pre-XDR-TB) and extensively drug-resistant TB (XDR-TB) has increased considerably worldwide....
| Hoofdauteurs: | , , , , , , , |
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| Formaat: | Artikel |
| Taal: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2020-05-01
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| Reeks: | Memorias do Instituto Oswaldo Cruz |
| Onderwerpen: | |
| Online toegang: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100315&tlng=en |
| _version_ | 1827847518155702272 |
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| author | Tania Matsui Juliana Maíra Watanabe Pinhata Michelle Christiane da Silva Rabello Angela Pires Brandão Lucilaine Ferrazoli Sylvia Cardoso Leão Cristina Viana-Niero Rosangela Siqueira de Oliveira |
| author_facet | Tania Matsui Juliana Maíra Watanabe Pinhata Michelle Christiane da Silva Rabello Angela Pires Brandão Lucilaine Ferrazoli Sylvia Cardoso Leão Cristina Viana-Niero Rosangela Siqueira de Oliveira |
| author_sort | Tania Matsui |
| collection | DOAJ |
| description | BACKGROUND Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis, and the number of new cases of multidrug resistant TB (MDR-TB), pre extensively drug-resistant TB (pre-XDR-TB) and extensively drug-resistant TB (XDR-TB) has increased considerably worldwide. OBJECTIVES Herein, using 156 M. tuberculosis isolates from 106 patients previously classified as MDR or pre-XDR or XDR isolates, we investigated the genetic mutation profiles associated with phenotypic resistances in patients with MDR-TB, pre-XDR-TB and XDR-TB, treatment outcomes and resistance evolution. METHODS Molecular analyses were performed by partial sequencing of the rpoB, katG, gyrA, gyrB, rrs genes and analysis of the fabG-inhA promoter region. Clinical, epidemiologic and demographic data were obtained from the TB Notification database system of São Paulo (TB-WEB) and the Information System for Special Tuberculosis Treatments (SITE-TB). FINDINGS Drug resistance was attributed to previously known mutations and a novel Asp449Val mutation in gyrB was observed in four isolates from the same patient. Ten patients had more than one isolate evaluated and eight of these patients displayed resistance progression. MAIN CONCLUSIONS The present study is the first to report the frequency of mutations related to second-line drug resistance in MDR-TB, pre-XDR-TB and XDR-TB isolates. The results could lead to the improvement of available technologies for the rapid detection of drug resistant TB. |
| first_indexed | 2024-03-12T09:31:24Z |
| format | Article |
| id | doaj.art-ab8bb62166b74a3984e88a0e3b6df35a |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:24Z |
| publishDate | 2020-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-ab8bb62166b74a3984e88a0e3b6df35a2023-09-02T13:54:13ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602020-05-0111510.1590/0074-02760200055Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, BrazilTania MatsuiJuliana Maíra Watanabe PinhataMichelle Christiane da Silva RabelloAngela Pires BrandãoLucilaine FerrazoliSylvia Cardoso LeãoCristina Viana-Nierohttps://orcid.org/0000-0002-1068-7884Rosangela Siqueira de OliveiraBACKGROUND Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis, and the number of new cases of multidrug resistant TB (MDR-TB), pre extensively drug-resistant TB (pre-XDR-TB) and extensively drug-resistant TB (XDR-TB) has increased considerably worldwide. OBJECTIVES Herein, using 156 M. tuberculosis isolates from 106 patients previously classified as MDR or pre-XDR or XDR isolates, we investigated the genetic mutation profiles associated with phenotypic resistances in patients with MDR-TB, pre-XDR-TB and XDR-TB, treatment outcomes and resistance evolution. METHODS Molecular analyses were performed by partial sequencing of the rpoB, katG, gyrA, gyrB, rrs genes and analysis of the fabG-inhA promoter region. Clinical, epidemiologic and demographic data were obtained from the TB Notification database system of São Paulo (TB-WEB) and the Information System for Special Tuberculosis Treatments (SITE-TB). FINDINGS Drug resistance was attributed to previously known mutations and a novel Asp449Val mutation in gyrB was observed in four isolates from the same patient. Ten patients had more than one isolate evaluated and eight of these patients displayed resistance progression. MAIN CONCLUSIONS The present study is the first to report the frequency of mutations related to second-line drug resistance in MDR-TB, pre-XDR-TB and XDR-TB isolates. The results could lead to the improvement of available technologies for the rapid detection of drug resistant TB.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100315&tlng=enMDR-TBpre-XDR-TBXDR-TBgyrAgyrBrrs |
| spellingShingle | Tania Matsui Juliana Maíra Watanabe Pinhata Michelle Christiane da Silva Rabello Angela Pires Brandão Lucilaine Ferrazoli Sylvia Cardoso Leão Cristina Viana-Niero Rosangela Siqueira de Oliveira Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil Memorias do Instituto Oswaldo Cruz MDR-TB pre-XDR-TB XDR-TB gyrA gyrB rrs |
| title | Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil |
| title_full | Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil |
| title_fullStr | Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil |
| title_full_unstemmed | Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil |
| title_short | Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil |
| title_sort | frequency of first and second line drug resistance associated mutations among resistant mycobacterium tuberculosis clinical isolates from sao paulo brazil |
| topic | MDR-TB pre-XDR-TB XDR-TB gyrA gyrB rrs |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100315&tlng=en |
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