Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-13843-6 |
| _version_ | 1818400481695760384 |
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| author | Namrata Ojha Kristin H. Rainey George H. Patterson |
| author_facet | Namrata Ojha Kristin H. Rainey George H. Patterson |
| author_sort | Namrata Ojha |
| collection | DOAJ |
| description | Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins. |
| first_indexed | 2024-12-14T07:37:16Z |
| format | Article |
| id | doaj.art-ab8c2a37ae65414495a695820bb8c70b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T07:37:16Z |
| publishDate | 2020-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ab8c2a37ae65414495a695820bb8c70b2022-12-21T23:11:08ZengNature PortfolioNature Communications2041-17232020-01-0111111110.1038/s41467-019-13843-6Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-associationNamrata Ojha0Kristin H. Rainey1George H. Patterson2Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthPerforming homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.https://doi.org/10.1038/s41467-019-13843-6 |
| spellingShingle | Namrata Ojha Kristin H. Rainey George H. Patterson Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association Nature Communications |
| title | Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association |
| title_full | Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association |
| title_fullStr | Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association |
| title_full_unstemmed | Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association |
| title_short | Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association |
| title_sort | imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self association |
| url | https://doi.org/10.1038/s41467-019-13843-6 |
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