Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli
Abstract Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-024-02317-0 |
| _version_ | 1827325319902658560 |
|---|---|
| author | Ping Li Mengjiao Wang Haiyan Di Qihang Du Yipeng Zhang Xiaoxu Tan Ping Xu Chao Gao Tianyi Jiang Chuanjuan Lü Cuiqing Ma |
| author_facet | Ping Li Mengjiao Wang Haiyan Di Qihang Du Yipeng Zhang Xiaoxu Tan Ping Xu Chao Gao Tianyi Jiang Chuanjuan Lü Cuiqing Ma |
| author_sort | Ping Li |
| collection | DOAJ |
| description | Abstract Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production. |
| first_indexed | 2024-03-07T14:25:26Z |
| format | Article |
| id | doaj.art-ab8e8022e24e4095ae90fcd35c9c6b12 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-07T14:25:26Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-ab8e8022e24e4095ae90fcd35c9c6b122024-03-06T08:06:19ZengBMCMicrobial Cell Factories1475-28592024-02-0123111110.1186/s12934-024-02317-0Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coliPing Li0Mengjiao Wang1Haiyan Di2Qihang Du3Yipeng Zhang4Xiaoxu Tan5Ping Xu6Chao Gao7Tianyi Jiang8Chuanjuan Lü9Cuiqing Ma10State Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityShandong Institute of MetrologyState Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Stomatology, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Technology, Shandong UniversitySchool of Municipal and Environmental Engineering, Shandong Jianzhu UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityAbstract Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production.https://doi.org/10.1186/s12934-024-02317-0Corn cob hydrolysate1,2,4-butanetriolXyloseMetabolic engineeringEscherichia coli |
| spellingShingle | Ping Li Mengjiao Wang Haiyan Di Qihang Du Yipeng Zhang Xiaoxu Tan Ping Xu Chao Gao Tianyi Jiang Chuanjuan Lü Cuiqing Ma Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli Microbial Cell Factories Corn cob hydrolysate 1,2,4-butanetriol Xylose Metabolic engineering Escherichia coli |
| title | Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli |
| title_full | Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli |
| title_fullStr | Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli |
| title_full_unstemmed | Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli |
| title_short | Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli |
| title_sort | efficient production of 1 2 4 butanetriol from corn cob hydrolysate by metabolically engineered escherichia coli |
| topic | Corn cob hydrolysate 1,2,4-butanetriol Xylose Metabolic engineering Escherichia coli |
| url | https://doi.org/10.1186/s12934-024-02317-0 |
| work_keys_str_mv | AT pingli efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT mengjiaowang efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT haiyandi efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT qihangdu efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT yipengzhang efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT xiaoxutan efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT pingxu efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT chaogao efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT tianyijiang efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT chuanjuanlu efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli AT cuiqingma efficientproductionof124butanetriolfromcorncobhydrolysatebymetabolicallyengineeredescherichiacoli |