Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging
Ultrasound contrast agents are valuable for diagnostic imaging and drug delivery. Generally, chemically synthesized microbubbles (MBs) are micro-sized particles. Particle size is a limiting factor for the diagnosis and treatment of many extravascular diseases. Recently, gas vesicles (GVs) from some...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/6/1198 |
| _version_ | 1797483347934445568 |
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| author | Mingjie Wei Manlin Lai Jiaqi Zhang Xiaoqing Pei Fei Yan |
| author_facet | Mingjie Wei Manlin Lai Jiaqi Zhang Xiaoqing Pei Fei Yan |
| author_sort | Mingjie Wei |
| collection | DOAJ |
| description | Ultrasound contrast agents are valuable for diagnostic imaging and drug delivery. Generally, chemically synthesized microbubbles (MBs) are micro-sized particles. Particle size is a limiting factor for the diagnosis and treatment of many extravascular diseases. Recently, gas vesicles (GVs) from some marine bacteria and archaea have been reported as novel nanoscale contrast agents, showing great potential for biomedical applications. However, most of the GVs reported in the literature show poor contrast imaging capabilities due to their small size, especially for the in vivo condition. In this study, we isolated the rugby-ball-shaped GVs from <i>Halobacteria NRC-1</i> and characterized their contrast imaging properties in vitro and in vivo. Our results showed that GVs could produce stable and strong ultrasound contrast signals in murine liver tumors using clinical diagnostic ultrasound equipment at the optimized parameters. Interestingly, we found these GVs, after systemic administration, were able to perfuse the ischemic region of a tumor where conventional lipid MBs failed, producing a 6.84-fold stronger contrast signal intensity than MBs. Immunohistochemistry staining assays revealed that the nanoscale GVs, in contrast to the microscale MBs, could penetrate through blood vessels. Thus, our study proved these biosynthesized GVs from <i>Halobacterium NRC-1</i> are useful for future molecular imaging and image-guided drug delivery. |
| first_indexed | 2024-03-09T22:45:44Z |
| format | Article |
| id | doaj.art-ab8f222ed29c45238bebe2e2f47e3008 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:45:44Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-ab8f222ed29c45238bebe2e2f47e30082023-11-23T18:29:50ZengMDPI AGPharmaceutics1999-49232022-06-01146119810.3390/pharmaceutics14061198Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor ImagingMingjie Wei0Manlin Lai1Jiaqi Zhang2Xiaoqing Pei3Fei Yan4Department of Ultrasound, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, ChinaDepartment of Ultrasound, The First Affiliated Hospital of Shenzhen University, Shenzhen 518061, ChinaDepartment of Ultrasound, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaDepartment of Ultrasound, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, ChinaCenter for Cell and Gene Circuit Design, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaUltrasound contrast agents are valuable for diagnostic imaging and drug delivery. Generally, chemically synthesized microbubbles (MBs) are micro-sized particles. Particle size is a limiting factor for the diagnosis and treatment of many extravascular diseases. Recently, gas vesicles (GVs) from some marine bacteria and archaea have been reported as novel nanoscale contrast agents, showing great potential for biomedical applications. However, most of the GVs reported in the literature show poor contrast imaging capabilities due to their small size, especially for the in vivo condition. In this study, we isolated the rugby-ball-shaped GVs from <i>Halobacteria NRC-1</i> and characterized their contrast imaging properties in vitro and in vivo. Our results showed that GVs could produce stable and strong ultrasound contrast signals in murine liver tumors using clinical diagnostic ultrasound equipment at the optimized parameters. Interestingly, we found these GVs, after systemic administration, were able to perfuse the ischemic region of a tumor where conventional lipid MBs failed, producing a 6.84-fold stronger contrast signal intensity than MBs. Immunohistochemistry staining assays revealed that the nanoscale GVs, in contrast to the microscale MBs, could penetrate through blood vessels. Thus, our study proved these biosynthesized GVs from <i>Halobacterium NRC-1</i> are useful for future molecular imaging and image-guided drug delivery.https://www.mdpi.com/1999-4923/14/6/1198ultrasound contrast agentsgas vesiclestumor imagingtumor’s ischemic region |
| spellingShingle | Mingjie Wei Manlin Lai Jiaqi Zhang Xiaoqing Pei Fei Yan Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging Pharmaceutics ultrasound contrast agents gas vesicles tumor imaging tumor’s ischemic region |
| title | Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging |
| title_full | Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging |
| title_fullStr | Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging |
| title_full_unstemmed | Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging |
| title_short | Biosynthetic Gas Vesicles from <i>Halobacteria NRC-1</i>: A Potential Ultrasound Contrast Agent for Tumor Imaging |
| title_sort | biosynthetic gas vesicles from i halobacteria nrc 1 i a potential ultrasound contrast agent for tumor imaging |
| topic | ultrasound contrast agents gas vesicles tumor imaging tumor’s ischemic region |
| url | https://www.mdpi.com/1999-4923/14/6/1198 |
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