Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups.
BACKGROUND: The mammary gland is a conserved site of lipoprotein lipase expression across species and lipoprotein lipase attachment to the luminal surface of mammary gland vascular endothelial cells has been implicated in the direction of circulating triglycerides into milk synthesis during lactatio...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3117854?pdf=render |
| _version_ | 1818587468192022528 |
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| author | Yuanyuan Wang Jia Tong Shuping Li Ran Zhang Li Chen Yuhui Wang Min Zheng Meili Wang George Liu Yunping Dai Yaofeng Zhao Ning Li |
| author_facet | Yuanyuan Wang Jia Tong Shuping Li Ran Zhang Li Chen Yuhui Wang Min Zheng Meili Wang George Liu Yunping Dai Yaofeng Zhao Ning Li |
| author_sort | Yuanyuan Wang |
| collection | DOAJ |
| description | BACKGROUND: The mammary gland is a conserved site of lipoprotein lipase expression across species and lipoprotein lipase attachment to the luminal surface of mammary gland vascular endothelial cells has been implicated in the direction of circulating triglycerides into milk synthesis during lactation. PRINCIPAL FINDINGS: Here we report generation of transgenic mice harboring a human lipoprotein lipase gene driven by a mammary gland-specific promoter. Lipoprotein lipase levels in transgenic milk was raised to 0.16 mg/ml, corresponding to an activity of 8772.95 mU/ml. High lipoprotein lipase activity led to a significant reduction of triglyceride concentration in milk, but other components were largely unchanged. Normal pups fed with transgenic milk showed inferior growth performances compared to those fed with normal milk. CONCLUSION: Our study suggests a possibility to reduce the triglyceride content of cow milk using transgenic technology. |
| first_indexed | 2024-12-16T09:09:20Z |
| format | Article |
| id | doaj.art-ab9846762ce34e2180708df56b73197f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:09:20Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ab9846762ce34e2180708df56b73197f2022-12-21T22:37:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2089510.1371/journal.pone.0020895Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups.Yuanyuan WangJia TongShuping LiRan ZhangLi ChenYuhui WangMin ZhengMeili WangGeorge LiuYunping DaiYaofeng ZhaoNing LiBACKGROUND: The mammary gland is a conserved site of lipoprotein lipase expression across species and lipoprotein lipase attachment to the luminal surface of mammary gland vascular endothelial cells has been implicated in the direction of circulating triglycerides into milk synthesis during lactation. PRINCIPAL FINDINGS: Here we report generation of transgenic mice harboring a human lipoprotein lipase gene driven by a mammary gland-specific promoter. Lipoprotein lipase levels in transgenic milk was raised to 0.16 mg/ml, corresponding to an activity of 8772.95 mU/ml. High lipoprotein lipase activity led to a significant reduction of triglyceride concentration in milk, but other components were largely unchanged. Normal pups fed with transgenic milk showed inferior growth performances compared to those fed with normal milk. CONCLUSION: Our study suggests a possibility to reduce the triglyceride content of cow milk using transgenic technology.http://europepmc.org/articles/PMC3117854?pdf=render |
| spellingShingle | Yuanyuan Wang Jia Tong Shuping Li Ran Zhang Li Chen Yuhui Wang Min Zheng Meili Wang George Liu Yunping Dai Yaofeng Zhao Ning Li Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. PLoS ONE |
| title | Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. |
| title_full | Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. |
| title_fullStr | Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. |
| title_full_unstemmed | Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. |
| title_short | Over-expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups. |
| title_sort | over expression of human lipoprotein lipase in mouse mammary glands leads to reduction of milk triglyceride and delayed growth of suckling pups |
| url | http://europepmc.org/articles/PMC3117854?pdf=render |
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