Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines
Objective To investigate the potential regualation of the expression of human maternal gene 3(MEG3) by transcription factor Kaiso and the impact on the occurrence and development of breast cancer. Methods The expression of Kaiso and MEG3 in breast cell line MCF-10a and different breast cancer cell l...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-05-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-5-758.pdf |
| _version_ | 1797365875002572800 |
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| author | XIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin |
| author_facet | XIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin |
| author_sort | XIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin |
| collection | DOAJ |
| description | Objective To investigate the potential regualation of the expression of human maternal gene 3(MEG3) by transcription factor Kaiso and the impact on the occurrence and development of breast cancer. Methods The expression of Kaiso and MEG3 in breast cell line MCF-10a and different breast cancer cell lines MCF-7,BT-474,MDA-MB-435,MDA-MB-231 were detected by RT-qPCR. The expression of Kaiso in cells was detected by Western blot.Kaiso expression of 50 samples from cancer and paracancer tissues were examined by Western blot. RT-qPCR was used to detect the effect of Kaiso over-expressed on the transcription of long non-coding RNA(lncRNA) MEG3. Results The transcription of lncRNA MEG3 in MCF-10a was higher than that of Kaiso (P＜0.001). Kaiso was significantly up-regulated in MCF-7, BT-474, MDA-MB-435, and MDA-MB-231 breast cancer cells,while lncRNA MEG3 was significantly down-regulated (P＜0.001). Kaiso expression level was low in MCF-10a, but was high in MDA-MB-231 highly metastatic breast cancer (P＜0.05). Kaiso was low in paracancerous breast tissues (P＜0.001), but highly expressed in cytoplasm and nucleus in cancer tissues. In addition, Kaiso over-expression in MCF-10a significantly down regulated the transcription of lncRNA MEG3(P＜0.01). Conclusions Kaiso regulates the expression of lncRNA MEG3 by breast cancer cells, which may play an important role in the occurrence and development of breast cancer. |
| first_indexed | 2024-03-08T16:56:06Z |
| format | Article |
| id | doaj.art-ab9a0bab5cfb450ebf21e68b26a4741c |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:56:06Z |
| publishDate | 2022-05-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-ab9a0bab5cfb450ebf21e68b26a4741c2024-01-05T02:35:09ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-05-0142575876210.16352/j.issn.1001-6325.2022.05.024Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell linesXIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin0Department of Clinical Biochemistry, Clinical Laboratory Medicine of Changsha Medical University, Changsha 410219,ChinaObjective To investigate the potential regualation of the expression of human maternal gene 3(MEG3) by transcription factor Kaiso and the impact on the occurrence and development of breast cancer. Methods The expression of Kaiso and MEG3 in breast cell line MCF-10a and different breast cancer cell lines MCF-7,BT-474,MDA-MB-435,MDA-MB-231 were detected by RT-qPCR. The expression of Kaiso in cells was detected by Western blot.Kaiso expression of 50 samples from cancer and paracancer tissues were examined by Western blot. RT-qPCR was used to detect the effect of Kaiso over-expressed on the transcription of long non-coding RNA(lncRNA) MEG3. Results The transcription of lncRNA MEG3 in MCF-10a was higher than that of Kaiso (P＜0.001). Kaiso was significantly up-regulated in MCF-7, BT-474, MDA-MB-435, and MDA-MB-231 breast cancer cells,while lncRNA MEG3 was significantly down-regulated (P＜0.001). Kaiso expression level was low in MCF-10a, but was high in MDA-MB-231 highly metastatic breast cancer (P＜0.05). Kaiso was low in paracancerous breast tissues (P＜0.001), but highly expressed in cytoplasm and nucleus in cancer tissues. In addition, Kaiso over-expression in MCF-10a significantly down regulated the transcription of lncRNA MEG3(P＜0.01). Conclusions Kaiso regulates the expression of lncRNA MEG3 by breast cancer cells, which may play an important role in the occurrence and development of breast cancer.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-5-758.pdfkaiso|long non-coding rna(lncrna)|maternally expressed gene 3(meg3)|breast cancer |
| spellingShingle | XIE Li-hua, YANG Qi, LI Ya-qian, XIE Xin-yue, LEI Shi-juan, ZHOU Lin Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines Jichu yixue yu linchuang kaiso|long non-coding rna(lncrna)|maternally expressed gene 3(meg3)|breast cancer |
| title | Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines |
| title_full | Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines |
| title_fullStr | Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines |
| title_full_unstemmed | Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines |
| title_short | Transcription factor Kaiso down-regulates the expression of lncRNA MEG3 in human breast cancer cell lines |
| title_sort | transcription factor kaiso down regulates the expression of lncrna meg3 in human breast cancer cell lines |
| topic | kaiso|long non-coding rna(lncrna)|maternally expressed gene 3(meg3)|breast cancer |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-5-758.pdf |
| work_keys_str_mv | AT xielihuayangqiliyaqianxiexinyueleishijuanzhoulin transcriptionfactorkaisodownregulatestheexpressionoflncrnameg3inhumanbreastcancercelllines |