Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery
ABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the deliv...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2018-04-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005007105&lng=en&tlng=en |
| _version_ | 1819084681077850112 |
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| author | DIEGO B. BORIN NATHANA J. MEZZOMO RODRIGO A. VAUCHER GUILHERME DO CARMO LUIZ C. RODRIGUES JUNIOR FERNANDO B. SULCZEWSKI CLAITON I. SCHWERTZ RICARDO E. MENDES ADRIANI P. DAMIANI VANESSA M. DE ANDRADE VIRGÍNIA C. RECH CARINA R. BOECK |
| author_facet | DIEGO B. BORIN NATHANA J. MEZZOMO RODRIGO A. VAUCHER GUILHERME DO CARMO LUIZ C. RODRIGUES JUNIOR FERNANDO B. SULCZEWSKI CLAITON I. SCHWERTZ RICARDO E. MENDES ADRIANI P. DAMIANI VANESSA M. DE ANDRADE VIRGÍNIA C. RECH CARINA R. BOECK |
| author_sort | DIEGO B. BORIN |
| collection | DOAJ |
| description | ABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine. |
| first_indexed | 2024-12-21T20:52:19Z |
| format | Article |
| id | doaj.art-ab9b815783d44c3cb9df37d35bb9fd1a |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-21T20:52:19Z |
| publishDate | 2018-04-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-ab9b815783d44c3cb9df37d35bb9fd1a2022-12-21T18:50:40ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902018-04-01010.1590/0001-3765201820170553S0001-37652018005007105Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain deliveryDIEGO B. BORINNATHANA J. MEZZOMORODRIGO A. VAUCHERGUILHERME DO CARMOLUIZ C. RODRIGUES JUNIORFERNANDO B. SULCZEWSKICLAITON I. SCHWERTZRICARDO E. MENDESADRIANI P. DAMIANIVANESSA M. DE ANDRADEVIRGÍNIA C. RECHCARINA R. BOECKABSTRACT Creatine acts intracellularly as energy buffer and storage, demonstrating protective effects in animal models of neurodegenerative diseases. However, its permeability throught blood-brain barrier (BBB) is reduced. The aim of the present study was developing a carrier to facilitate the delivery of creatine to the central nervous system. Creatine nanoliposomes were produced, characterized and assayed in models of toxicity in vitro and in vivo. Particles showed negative zeta potential (-12,5 mV), polydispersity index 0.237 and medium-size of 105 nm, which was confirmed by transmission electron microscopy (TEM) images. Toxicity assay in vitro was evaluated with blank liposomes (no drug) or creatine nanoliposomes at concentrations of 0.02 and 0.2 mg/mL, that did not influence the viability of Vero cells. The result. of the comet assay that the nanoliposomes are not genotoxic, togeher with cell viability demonstrated that the nanoliposomes are not toxic. Besides, in vivo assays not demonstrate toxicity in hematological and biochemical markers of young rats. Nevertheless, increase content of creatine in the cerebral cortex tissue after subchronic treatment was observed. Altogether, results indicate increase permeability of creatine to the BBB that could be used as assay for in vivo studies to confirm improved effect than free creatine.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005007105&lng=en&tlng=ennanocarrierneurodegenerative diseasesnanotechnologyliposomes |
| spellingShingle | DIEGO B. BORIN NATHANA J. MEZZOMO RODRIGO A. VAUCHER GUILHERME DO CARMO LUIZ C. RODRIGUES JUNIOR FERNANDO B. SULCZEWSKI CLAITON I. SCHWERTZ RICARDO E. MENDES ADRIANI P. DAMIANI VANESSA M. DE ANDRADE VIRGÍNIA C. RECH CARINA R. BOECK Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery Anais da Academia Brasileira de Ciências nanocarrier neurodegenerative diseases nanotechnology liposomes |
| title | Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| title_full | Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| title_fullStr | Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| title_full_unstemmed | Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| title_short | Production, characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| title_sort | production characterization and toxicology assay of creatine pegylated nanoliposome with polysorbate 80 for brain delivery |
| topic | nanocarrier neurodegenerative diseases nanotechnology liposomes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018005007105&lng=en&tlng=en |
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