The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma
IntroductionGelsemium elegans (G. elegans) as a traditional medicinal plant used in livestock production. The use of G. elegans in veterinary clinics may pose safety risks to human health.ObjectivesThe aim of this study was to investigate tissue residue depletion in pigs fed G. elegans powder.Method...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1111782/full |
| _version_ | 1797953967662039040 |
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| author | Yong Wu Xue-Ming Long Gao-Feng Liu Xia Bai Zhi-Liang Sun Zhao-Ying Liu |
| author_facet | Yong Wu Xue-Ming Long Gao-Feng Liu Xia Bai Zhi-Liang Sun Zhao-Ying Liu |
| author_sort | Yong Wu |
| collection | DOAJ |
| description | IntroductionGelsemium elegans (G. elegans) as a traditional medicinal plant used in livestock production. The use of G. elegans in veterinary clinics may pose safety risks to human health.ObjectivesThe aim of this study was to investigate tissue residue depletion in pigs fed G. elegans powder.MethodsA precise quantitation method and a simultaneous semi-quantitation method for multiple components independently of standards in pig tissues were developed for the first time. The two methods were validated in terms of specificity, LODs, LOQs, linearity, accuracy, precision, and matrix effects. They were then applied to a tissue residue depletion study after G. elegans powder at a dose of 2% per kg feed were fed to pigs.ResultsCompared with precise quantitation, the method validation results indicated that the semi-quantitation method was reliable and acceptable for multicomponent quantification independent of standards. Many G. elegans alkaloids are widely distributed in most tissues of pigs. Tissue residue depletion studies indicated that 14-hydroxygelsenicine, 11-hydroxygelsenicine, and gelsemoxonine could be used as potential residue markers, and pancreas, small intestine, and lung tissues could be considered as potential residue target tissues of G. elegans. In addition, both urine and plasma could be used to predict 14-hydroxygelsenicine and gelsemoxonine residues in the liver, pancreas, and small intestinal tissues of pigs.ConclusionThe developed semi-quantification method can be applied to monitor the application and residue of G. elegans. The results provide scientific evidence for evaluating the safety of animal-derived food from G. elegans for consumers and will be helpful for its application and future development. |
| first_indexed | 2024-04-10T23:11:24Z |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T23:11:24Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ab9ba2f52c38457a93751790816daf5b2023-01-13T05:34:47ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-01-01910.3389/fvets.2022.11117821111782The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasmaYong Wu0Xue-Ming Long1Gao-Feng Liu2Xia Bai3Zhi-Liang Sun4Zhao-Ying Liu5College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, ChinaHunan Provincial Institute of Veterinary Drugs and Feed Control, Changsha, Hunan, ChinaHunan Canzoho Biological Technology Co., Ltd., Liuyang, Hunan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, ChinaIntroductionGelsemium elegans (G. elegans) as a traditional medicinal plant used in livestock production. The use of G. elegans in veterinary clinics may pose safety risks to human health.ObjectivesThe aim of this study was to investigate tissue residue depletion in pigs fed G. elegans powder.MethodsA precise quantitation method and a simultaneous semi-quantitation method for multiple components independently of standards in pig tissues were developed for the first time. The two methods were validated in terms of specificity, LODs, LOQs, linearity, accuracy, precision, and matrix effects. They were then applied to a tissue residue depletion study after G. elegans powder at a dose of 2% per kg feed were fed to pigs.ResultsCompared with precise quantitation, the method validation results indicated that the semi-quantitation method was reliable and acceptable for multicomponent quantification independent of standards. Many G. elegans alkaloids are widely distributed in most tissues of pigs. Tissue residue depletion studies indicated that 14-hydroxygelsenicine, 11-hydroxygelsenicine, and gelsemoxonine could be used as potential residue markers, and pancreas, small intestine, and lung tissues could be considered as potential residue target tissues of G. elegans. In addition, both urine and plasma could be used to predict 14-hydroxygelsenicine and gelsemoxonine residues in the liver, pancreas, and small intestinal tissues of pigs.ConclusionThe developed semi-quantification method can be applied to monitor the application and residue of G. elegans. The results provide scientific evidence for evaluating the safety of animal-derived food from G. elegans for consumers and will be helpful for its application and future development.https://www.frontiersin.org/articles/10.3389/fvets.2022.1111782/fullGelsemiumLC-MS/MSresidue depletiongelsedinetarget tissue |
| spellingShingle | Yong Wu Xue-Ming Long Gao-Feng Liu Xia Bai Zhi-Liang Sun Zhao-Ying Liu The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma Frontiers in Veterinary Science Gelsemium LC-MS/MS residue depletion gelsedine target tissue |
| title | The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma |
| title_full | The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma |
| title_fullStr | The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma |
| title_full_unstemmed | The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma |
| title_short | The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma |
| title_sort | multicomponent residue depletion of gelsemium elegans in pig tissues urine and plasma |
| topic | Gelsemium LC-MS/MS residue depletion gelsedine target tissue |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1111782/full |
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