Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications
Abstract In this research work, carbon dots (CDs) were synthesized from the renewable leaves of an indigenous medicinal plant by the one-pot sand bath method, Azadirachta indica. The synthesized CDs were characterized for its optical properties using UV–Vis, Fluorescence and Fourier transform infrar...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33652-8 |
| _version_ | 1797841026919956480 |
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| author | Gangaraju Gedda Sri Amruthaa Sankaranarayanan Chandra Lekha Putta Krishna Kanthi Gudimella Aravind Kumar Rengan Wubshet Mekonnen Girma |
| author_facet | Gangaraju Gedda Sri Amruthaa Sankaranarayanan Chandra Lekha Putta Krishna Kanthi Gudimella Aravind Kumar Rengan Wubshet Mekonnen Girma |
| author_sort | Gangaraju Gedda |
| collection | DOAJ |
| description | Abstract In this research work, carbon dots (CDs) were synthesized from the renewable leaves of an indigenous medicinal plant by the one-pot sand bath method, Azadirachta indica. The synthesized CDs were characterized for its optical properties using UV–Vis, Fluorescence and Fourier transform infrared (FT-IR) spectrophotometry and for structural properties using dynamic light scattering (DLS), X-ray Diffraction (XRD) and high resolution Transmission electron microscopy (HR-TEM). The synthesized CDs exhibited concentration dependent biocompatibility when tested in mouse fibroblast L929 cell line. The EC50 values of biomedical studies, free radical scavenging activity (13.87 μgmL−1), and total antioxidant capacity (38 μgmL−1) proved CDs were exceptionally good. These CDs showed an appreciable zone of inhibition when examined on four bacterial (two gram-positive and gram-negative) and two fungal strains at minimum concentrations. Cellular internalisation studies performed on human breast cancer cells (MCF 7- bioimaging) revealed the applicability of CDs in bioimaging, wherein the inherent fluorescence of CDs were utilised. Thus, the CDs developed are potential as bioimaging, antioxidants and antimicrobial agents. |
| first_indexed | 2024-04-09T16:24:12Z |
| format | Article |
| id | doaj.art-ab9e9170138a4e7f920be27770a5d720 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T16:24:12Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ab9e9170138a4e7f920be27770a5d7202023-04-23T11:17:07ZengNature PortfolioScientific Reports2045-23222023-04-011311910.1038/s41598-023-33652-8Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applicationsGangaraju Gedda0Sri Amruthaa Sankaranarayanan1Chandra Lekha Putta2Krishna Kanthi Gudimella3Aravind Kumar Rengan4Wubshet Mekonnen Girma5Department of Chemistry, School of Engineering, Presidency UniversityDepartment of Biomedical Engineering, Indian Institute of Technology HyderabadDepartment of Biomedical Engineering, Indian Institute of Technology HyderabadDepartment of Chemistry, School of Science, GITAM (Deemed to Be University)Department of Biomedical Engineering, Indian Institute of Technology HyderabadDepartment of Chemistry, College of Natural Science, Wollo UniversityAbstract In this research work, carbon dots (CDs) were synthesized from the renewable leaves of an indigenous medicinal plant by the one-pot sand bath method, Azadirachta indica. The synthesized CDs were characterized for its optical properties using UV–Vis, Fluorescence and Fourier transform infrared (FT-IR) spectrophotometry and for structural properties using dynamic light scattering (DLS), X-ray Diffraction (XRD) and high resolution Transmission electron microscopy (HR-TEM). The synthesized CDs exhibited concentration dependent biocompatibility when tested in mouse fibroblast L929 cell line. The EC50 values of biomedical studies, free radical scavenging activity (13.87 μgmL−1), and total antioxidant capacity (38 μgmL−1) proved CDs were exceptionally good. These CDs showed an appreciable zone of inhibition when examined on four bacterial (two gram-positive and gram-negative) and two fungal strains at minimum concentrations. Cellular internalisation studies performed on human breast cancer cells (MCF 7- bioimaging) revealed the applicability of CDs in bioimaging, wherein the inherent fluorescence of CDs were utilised. Thus, the CDs developed are potential as bioimaging, antioxidants and antimicrobial agents.https://doi.org/10.1038/s41598-023-33652-8 |
| spellingShingle | Gangaraju Gedda Sri Amruthaa Sankaranarayanan Chandra Lekha Putta Krishna Kanthi Gudimella Aravind Kumar Rengan Wubshet Mekonnen Girma Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications Scientific Reports |
| title | Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications |
| title_full | Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications |
| title_fullStr | Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications |
| title_full_unstemmed | Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications |
| title_short | Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications |
| title_sort | green synthesis of multi functional carbon dots from medicinal plant leaves for antimicrobial antioxidant and bioimaging applications |
| url | https://doi.org/10.1038/s41598-023-33652-8 |
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