miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction
Long-lived HIV-1 reservoirs include tissue macrophages. Monocyte-derived macrophages are more susceptible to infection and more permissive to HIV-1 replication than monocytes for reasons that may include the effects of different populations of miRNAs in these two cell classes. Specifically, miRs-28-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-09-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/4/10/1844 |
| _version_ | 1819208194318139392 |
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| author | Janice E. Clements Jeanne M. Sisk Kenneth W. Witwer |
| author_facet | Janice E. Clements Jeanne M. Sisk Kenneth W. Witwer |
| author_sort | Janice E. Clements |
| collection | DOAJ |
| description | Long-lived HIV-1 reservoirs include tissue macrophages. Monocyte-derived macrophages are more susceptible to infection and more permissive to HIV-1 replication than monocytes for reasons that may include the effects of different populations of miRNAs in these two cell classes. Specifically, miRs-28-3p, -150, -223, -198, and -382 exert direct or indirect negative effects on HIV-1 and are reportedly downmodulated during monocyte-to-macrophage differentiation. Here, new experimental results are presented along with reviews and analysis of published studies and publicly available datasets, supporting a broader role of miRNAs in HIV-1 restriction than would be suggested by a simple and uniform downregulation of anti-HIV miRNAs during monocyte-to-macrophage differentiation. Although miR-223 is downregulated in macrophages, other putatively antiviral miRNAs are more abundant in macrophages than in monocytes or are rare and/or variably present in both cell classes. Our analyses point to the need for further studies to determine miRNA profiles of monocytes and macrophages, including classic and newly identified subpopulations; examine the sensitivity of miRNA profiling to cell isolation and differentiation protocols; and characterize rigorously the antiviral effects of previously reported and novel predicted miRNA-HIV-1 interactions in cell-specific contexts. |
| first_indexed | 2024-12-23T05:35:31Z |
| format | Article |
| id | doaj.art-ab9f3dda1d644d75895813c9c970401c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T05:35:31Z |
| publishDate | 2012-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ab9f3dda1d644d75895813c9c970401c2022-12-21T17:58:21ZengMDPI AGViruses1999-49152012-09-014101844186410.3390/v4101844miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 RestrictionJanice E. ClementsJeanne M. SiskKenneth W. WitwerLong-lived HIV-1 reservoirs include tissue macrophages. Monocyte-derived macrophages are more susceptible to infection and more permissive to HIV-1 replication than monocytes for reasons that may include the effects of different populations of miRNAs in these two cell classes. Specifically, miRs-28-3p, -150, -223, -198, and -382 exert direct or indirect negative effects on HIV-1 and are reportedly downmodulated during monocyte-to-macrophage differentiation. Here, new experimental results are presented along with reviews and analysis of published studies and publicly available datasets, supporting a broader role of miRNAs in HIV-1 restriction than would be suggested by a simple and uniform downregulation of anti-HIV miRNAs during monocyte-to-macrophage differentiation. Although miR-223 is downregulated in macrophages, other putatively antiviral miRNAs are more abundant in macrophages than in monocytes or are rare and/or variably present in both cell classes. Our analyses point to the need for further studies to determine miRNA profiles of monocytes and macrophages, including classic and newly identified subpopulations; examine the sensitivity of miRNA profiling to cell isolation and differentiation protocols; and characterize rigorously the antiviral effects of previously reported and novel predicted miRNA-HIV-1 interactions in cell-specific contexts.http://www.mdpi.com/1999-4915/4/10/1844microRNAHIV-1monocytemacrophageprofilingantiviral |
| spellingShingle | Janice E. Clements Jeanne M. Sisk Kenneth W. Witwer miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction Viruses microRNA HIV-1 monocyte macrophage profiling antiviral |
| title | miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction |
| title_full | miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction |
| title_fullStr | miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction |
| title_full_unstemmed | miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction |
| title_short | miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction |
| title_sort | mirna profiles of monocyte lineage cells are consistent with complicated roles in hiv 1 restriction |
| topic | microRNA HIV-1 monocyte macrophage profiling antiviral |
| url | http://www.mdpi.com/1999-4915/4/10/1844 |
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