An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆
A highly sensitive and selective high performance liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of alverine (ALV) and its active metabolite, para hydroxy alverine (PHA), in human plasma. For sample preparation, solid phase extraction of anal...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177916301228 |
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| author | Dhiraj M. Rathod Keyur R. Patel Hiren N. Mistri Arvind G. Jangid Pranav S. Shrivastav Mallika Sanyal |
| author_facet | Dhiraj M. Rathod Keyur R. Patel Hiren N. Mistri Arvind G. Jangid Pranav S. Shrivastav Mallika Sanyal |
| author_sort | Dhiraj M. Rathod |
| collection | DOAJ |
| description | A highly sensitive and selective high performance liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of alverine (ALV) and its active metabolite, para hydroxy alverine (PHA), in human plasma. For sample preparation, solid phase extraction of analytes was performed on Phenomenex Strata-X cartridges using alverine-d5 as the internal standard. The analytes were separated on Symmetry Shield RP18 (150 mm×3.9 mm, 5 µm) column with a mobile phase consisting of acetonitrile and 10 mM ammonium formate (65:35, v/v). Detection and quantitation was done by electrospray ionization mass spectrometry in the positive mode using multiple reaction monitoring. The assay method was fully validated over the concentration range of 15.0–15,000 pg/mL for ALV and 30.0–15,000 pg/mL for PHA. The intra-day and inter-day accuracy and precision (% CV) ranged from 94.00% to 96.00% and 0.48% to 4.15% for both the analytes. The mean recovery obtained for ALV and PHA was 80.59% and 81.26%, respectively. Matrix effect, expressed as IS-normalized matrix factor ranged from 0.982 to 1.009 for both the analytes. The application of the method was demonstrated for the specific analysis of ALV and PHA for a bioequivalence study in 52 healthy subjects using 120 mg ALV capsules. The assay reproducibility was also verified by reanalysis of 175 incurred subject samples. |
| first_indexed | 2024-12-17T22:11:53Z |
| format | Article |
| id | doaj.art-ab9f7dc3edbe4ac790f752b4c71282e1 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-17T22:11:53Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-ab9f7dc3edbe4ac790f752b4c71282e12022-12-21T21:30:44ZengElsevierJournal of Pharmaceutical Analysis2095-17792017-04-01729510210.1016/j.jpha.2016.11.003An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆Dhiraj M. Rathod0Keyur R. Patel1Hiren N. Mistri2Arvind G. Jangid3Pranav S. Shrivastav4Mallika Sanyal5Department of Chemistry, Kadi Sarva Vishwavidyalaya, Gandhinagar, Ahmedabad-382015, IndiaBioanalytical Research Department, Accutest Research Lab, Bodakdev, Ahmedabad-380059, IndiaBioanalytical Research Department, Accutest Research Lab, Bodakdev, Ahmedabad-380059, IndiaBioanalytical Research Department, Accutest Research Lab, Bodakdev, Ahmedabad-380059, IndiaDepartment of Chemistry, School of Sciences, Gujarat University, Ahmedabad-380009, IndiaDepartment of Chemistry, St. Xavier's College, Navrangpura, Ahmedabad-380009, IndiaA highly sensitive and selective high performance liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of alverine (ALV) and its active metabolite, para hydroxy alverine (PHA), in human plasma. For sample preparation, solid phase extraction of analytes was performed on Phenomenex Strata-X cartridges using alverine-d5 as the internal standard. The analytes were separated on Symmetry Shield RP18 (150 mm×3.9 mm, 5 µm) column with a mobile phase consisting of acetonitrile and 10 mM ammonium formate (65:35, v/v). Detection and quantitation was done by electrospray ionization mass spectrometry in the positive mode using multiple reaction monitoring. The assay method was fully validated over the concentration range of 15.0–15,000 pg/mL for ALV and 30.0–15,000 pg/mL for PHA. The intra-day and inter-day accuracy and precision (% CV) ranged from 94.00% to 96.00% and 0.48% to 4.15% for both the analytes. The mean recovery obtained for ALV and PHA was 80.59% and 81.26%, respectively. Matrix effect, expressed as IS-normalized matrix factor ranged from 0.982 to 1.009 for both the analytes. The application of the method was demonstrated for the specific analysis of ALV and PHA for a bioequivalence study in 52 healthy subjects using 120 mg ALV capsules. The assay reproducibility was also verified by reanalysis of 175 incurred subject samples.http://www.sciencedirect.com/science/article/pii/S2095177916301228AlverinePara hydroxy alverineLiquid chromatography-tandem mass spectrometryBioequivalence studyHuman plasma |
| spellingShingle | Dhiraj M. Rathod Keyur R. Patel Hiren N. Mistri Arvind G. Jangid Pranav S. Shrivastav Mallika Sanyal An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ Journal of Pharmaceutical Analysis Alverine Para hydroxy alverine Liquid chromatography-tandem mass spectrometry Bioequivalence study Human plasma |
| title | An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| title_full | An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| title_fullStr | An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| title_full_unstemmed | An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| title_short | An improved LC-MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| title_sort | improved lc ms ms method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study☆ |
| topic | Alverine Para hydroxy alverine Liquid chromatography-tandem mass spectrometry Bioequivalence study Human plasma |
| url | http://www.sciencedirect.com/science/article/pii/S2095177916301228 |
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