The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model
More than 30% of all human malignancies are brought about by mutations in RAS proto-oncogenes (HRAS, KRAS, and NRAS) that are greatly conserved in yeast <i>RAS1</i> and <i>RAS2</i>. This makes yeast (<i>Saccharomyces cerevisiae</i>) an efficient single-celled euka...
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MDPI AG
2023-03-01
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| Series: | Biology and Life Sciences Forum |
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| Online Access: | https://www.mdpi.com/2673-9976/21/1/9 |
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| author | Nazela Ahmed Chowdhury Fatema Akter Sadia Ismat Jahan Anee Ashfaqul Muid Khandaker |
| author_facet | Nazela Ahmed Chowdhury Fatema Akter Sadia Ismat Jahan Anee Ashfaqul Muid Khandaker |
| author_sort | Nazela Ahmed Chowdhury |
| collection | DOAJ |
| description | More than 30% of all human malignancies are brought about by mutations in RAS proto-oncogenes (HRAS, KRAS, and NRAS) that are greatly conserved in yeast <i>RAS1</i> and <i>RAS2</i>. This makes yeast (<i>Saccharomyces cerevisiae</i>) an efficient single-celled eukaryotic model organism to study their functions. In the current investigation, the null mutation of the <i>RAS2</i> gene was analyzed to find out its deleterious consequences in yeast cells based on their ability to utilize glycerol as a respiratory substrate, mtDNA mutation rate, mtDNA abundance, and distribution pattern. Mutant cells grown in YPEG plates demonstrated slight respiratory deficiency compared to the wild type. An erythromycin-resistant assay was carried out to analyze the spontaneous mitochondrial DNA mutation rate in the Δ<i>ras2</i> mutant and it was found to be greater than that of wild type. In addition, the mitochondrial DNAs of both strains were also visualized under a fluorescence microscope using DAPI fluorescent stain. It was observed that mtDNA abundance was much lower compared to wild type cells. Thus, the present investigation revealed that deletion of the <i>RAS2</i> gene resulted in mtDNA mutation and depletion. |
| first_indexed | 2024-03-11T02:42:03Z |
| format | Article |
| id | doaj.art-aba0ff9dd0ae4b98bdff52e3aa4986cd |
| institution | Directory Open Access Journal |
| issn | 2673-9976 |
| language | English |
| last_indexed | 2024-03-11T02:42:03Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-aba0ff9dd0ae4b98bdff52e3aa4986cd2023-11-18T09:33:55ZengMDPI AGBiology and Life Sciences Forum2673-99762023-03-01211910.3390/blsf2023021009The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast ModelNazela Ahmed Chowdhury0Fatema Akter Sadia1Ismat Jahan Anee2Ashfaqul Muid Khandaker3Branch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Dhaka 1000, BangladeshBranch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Dhaka 1000, BangladeshBranch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Dhaka 1000, BangladeshBranch of Genetics and Molecular Biology, Department of Zoology, University of Dhaka, Dhaka 1000, BangladeshMore than 30% of all human malignancies are brought about by mutations in RAS proto-oncogenes (HRAS, KRAS, and NRAS) that are greatly conserved in yeast <i>RAS1</i> and <i>RAS2</i>. This makes yeast (<i>Saccharomyces cerevisiae</i>) an efficient single-celled eukaryotic model organism to study their functions. In the current investigation, the null mutation of the <i>RAS2</i> gene was analyzed to find out its deleterious consequences in yeast cells based on their ability to utilize glycerol as a respiratory substrate, mtDNA mutation rate, mtDNA abundance, and distribution pattern. Mutant cells grown in YPEG plates demonstrated slight respiratory deficiency compared to the wild type. An erythromycin-resistant assay was carried out to analyze the spontaneous mitochondrial DNA mutation rate in the Δ<i>ras2</i> mutant and it was found to be greater than that of wild type. In addition, the mitochondrial DNAs of both strains were also visualized under a fluorescence microscope using DAPI fluorescent stain. It was observed that mtDNA abundance was much lower compared to wild type cells. Thus, the present investigation revealed that deletion of the <i>RAS2</i> gene resulted in mtDNA mutation and depletion.https://www.mdpi.com/2673-9976/21/1/9mutationRAS proto-oncogenes<i>S. cerevisiae</i>mitochondrial DNAcancer |
| spellingShingle | Nazela Ahmed Chowdhury Fatema Akter Sadia Ismat Jahan Anee Ashfaqul Muid Khandaker The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model Biology and Life Sciences Forum mutation RAS proto-oncogenes <i>S. cerevisiae</i> mitochondrial DNA cancer |
| title | The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model |
| title_full | The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model |
| title_fullStr | The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model |
| title_full_unstemmed | The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model |
| title_short | The Effect of <i>RAS2</i> Gene Mutation in Single Cell Yeast Model |
| title_sort | effect of i ras2 i gene mutation in single cell yeast model |
| topic | mutation RAS proto-oncogenes <i>S. cerevisiae</i> mitochondrial DNA cancer |
| url | https://www.mdpi.com/2673-9976/21/1/9 |
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